Population structure, virulence potential and antibiotic susceptibility of uropathogenic Escherichia coli from Northwest England

Gibreel, Tarek M.; Dodgson, Andrew; Cheesbrough, J.; Fox, Andrew; Bolton, F. J.; Upton, Mathew

doi:10.1093/jac/dkr451

Cited by 160 publications

(204 citation statements)

References 44 publications

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“…29 Over the last decade, the ST131 virulent pandemic clone has emerged in hospitals and in the community worldwide, causing outbreaks of MDR infections across the globe. 30 It has been frequently described as a urinary pathogen in humans, 31 and a fatal case of urosepsis associated with a multi-resistant strain of E. coli ST131 was reported recently 30 Although rarely isolated from companion animals, a recent report found E. coli ST131 to distribute among human and animal household members in Australia, suggesting serious clinical and public health implications. 32 In Europe, until now, ST131 has only been isolated from dogs, horses, 33 poultry 34 and pigs 35 .…”

Section: Discussionmentioning

confidence: 99%

Genetic and phenotypic characterisation of Escherichia coli producing cefotaximase-type extended-spectrum β-lactamases: first evidence of the ST131 clone in cats with urinary infections in Italy

Nebbia

Tramuta

Odore

et al. 2014

Journal of Feline Medicine and Surgery

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The incidence of cefotaximase (CTX-M)-type extended-spectrum β-lactamase (ESBL)-producing Escherichia coli has increased dramatically in humans and animals since the middle of the last century. E coli that produce CTX-M β-lactamase represent a major cause of urinary tract infections, and pose a significant therapeutic challenge to both human and veterinary medicine. As data on uropathogenic CTX-M-producing strains in cats are limited, the aim of this study was to describe the genetic character and antibiotic resistance phenotypes of CTX-M-producing E coli isolated from cats with cystitis. Seven of 15 E coli bacteria isolated from 138 urine samples had the CTX-M gene and were therefore included in this study. These isolates were screened by polymerase chain reaction for the presence of 14 extra-intestinal virulence factors, class 1 and class 2 integrons, and to identify their phylogenetic groups. Multi-locus sequence typing (MLST) of the strains and susceptibility testing (disc diffusion method) were also performed. Virulence factor iutA was the most frequent determinant identified (86.7%), and the majority of CTX-M-producing strains (n = 5) carried class 1 integrons. MLST allowed us to discriminate four known sequence types (ST131, ST555, ST602, ST155) and three novel sequence types (ST3847, ST3848, ST4181). To the best of our knowledge, this is the first study to report uropathogenic CTX-M-producing E coli ST131 in cats in Italy. Accurate diagnostics and prudent use of antimicrobials are recommended to avoid the spread of multidrug-resistant pathogens in veterinary medicine and to prevent their transmission to humans.

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Section: Discussionmentioning

confidence: 99%

Genetic and phenotypic characterisation of Escherichia coli producing cefotaximase-type extended-spectrum β-lactamases: first evidence of the ST131 clone in cats with urinary infections in Italy

Nebbia

Tramuta

Odore

et al. 2014

Journal of Feline Medicine and Surgery

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“…Previous genetic studies of E. coli lineages associated with UTI and/or bacteremia in England and the US have reported that the most prevalent multilocus sequence types (MLSTs) are sequence types (STs) ST73, ST131, ST95, and ST69 (Gibreel et al 2012;Adams-Sapper et al 2013;Alhashash et al 2013;Banerjee et al 2013;Horner et al 2014). ST131 has received particular attention, following its apparent emergence in the 2000s, due to its rapid global dissemination and frequent multidrug-resistant (MDR) phenotype (Nicolas-Chanoine et al 2014).…”

Section: [Supplemental Materials Is Available For This Article]mentioning

confidence: 99%

Systematic longitudinal survey of invasive Escherichia coli in England demonstrates a stable population structure only transiently disturbed by the emergence of ST131

et al. 2017

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Escherichia coli associated with urinary tract infections and bacteremia has been intensively investigated, including recent work focusing on the virulent, globally disseminated, multidrug-resistant lineage ST131. To contextualize ST131 within the broader E. coli population associated with disease, we used genomics to analyze a systematic 11-yr hospital-based survey of E. coli associated with bacteremia using isolates collected from across England by the British Society for Antimicrobial Chemotherapy and from the Cambridge University Hospitals NHS Foundation Trust. Population dynamics analysis of the most successful lineages identified the emergence of ST131 and ST69 and their establishment as two of the five most common lineages along with ST73, ST95, and ST12. The most frequently identified lineage was ST73. Compared to ST131, ST73 was susceptible to most antibiotics, indicating that multidrug resistance was not the dominant reason for prevalence of E. coli lineages in this population. Temporal phylogenetic analysis of the emergence of ST69 and ST131 identified differences in the dynamics of emergence and showed that expansion of ST131 in this population was not driven by sequential emergence of increasingly resistant subclades. We showed that over time, the E. coli population was only transiently disturbed by the introduction of new lineages before a new equilibrium was rapidly achieved. Together, these findings suggest that the frequency of E. coli lineages in invasive disease is driven by negative frequency-dependent selection occurring outside of the hospital, most probably in the commensal niche, and that drug resistance is not a primary determinant of success in this niche.

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“…Among VF genes, iroN and sfa were significantly more present in VAP isolates than colonization isolates (Table 3). Further characterization of the 16 VAP B2 isolates showed that only two belonged to the frequently recovered subgroup II [multilocus sequence type complex (STc)73)] [27]. Of note, five isolates from four patients belonged to subgroup III (STc127), which encompasses the highly virulent archetypal strain 536 [26] (Table 4).…”

Section: Phylogenetic Groups and Vf Genesmentioning

confidence: 99%

Pathophysiology of Escherichia coli ventilator-associated pneumonia: implication of highly virulent extraintestinal pathogenic strains

et al. 2012

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Population structure, virulence potential and antibiotic susceptibility of uropathogenic Escherichia coli from Northwest England

Abstract: ST131 isolates are well established in the wider UPEC population. This clone is still evolving and we further support suggestions that it represents a real threat to health. We suggest that ST127 is a recently emerged, community-associated, virulent clone that warrants further study.

Cited by 160 publications

References 44 publications

Genetic and phenotypic characterisation of Escherichia coli producing cefotaximase-type extended-spectrum β-lactamases: first evidence of the ST131 clone in cats with urinary infections in Italy

Genetic and phenotypic characterisation of Escherichia coli producing cefotaximase-type extended-spectrum β-lactamases: first evidence of the ST131 clone in cats with urinary infections in Italy

Systematic longitudinal survey of invasive Escherichia coli in England demonstrates a stable population structure only transiently disturbed by the emergence of ST131

Pathophysiology of Escherichia coli ventilator-associated pneumonia: implication of highly virulent extraintestinal pathogenic strains

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