Porcine Fetal Enamel Matrix Derivative Enhances Bone Formation Induced by Demineralized Freeze Dried Bone Allograft In Vivo

Boyan, Barbara D.; Weesner, Theodore C.; Lohmann, Christoph H.; Andreacchio, D.; Carnes, David L.; Dean, David D.; Cochran, David L.; Schwartz, Zvi

doi:10.1902/jop.2000.71.8.1278

Cited by 161 publications

(144 citation statements)

References 38 publications

Supporting

Mentioning

133

Contrasting

Unclassified

Order By: Relevance

“…DEXA method may be used to measure tooth mineral density (TMD) at the particular region of interest (ROI) of sample or for the whole sample. The results of measurements are given in mineral density expressed as g/ cm 2 . Apart from the TMD, DEXA allows the measurement of tooth mineral content (TMC), which is expressed in grams.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Interrelationships between Tooth Properties and Biochemical Bone Turnover Markers Investigated on Six-Month-Old Pig Model

Tymczyna

Tatara

Krupski

et al. 2013

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

ABSTRACT. The aim of the study was to determine interrelationships between bone tissue metabolism indices and morphological, biomechanical and densitometric properties of hard dental tissues. First primary maxillary incisor from 6-month-old pigs (N=27) was evaluated in terms of weight and length. Mean volumetric tooth mineral density, total tooth volume, enamel total volume, enamel volumetric mineral density, dentine total volume and dentine volumetric mineral density were estimated with the use of quantitative computed tomography and micro computed tomography techniques. Tooth mineral density and tooth mineral content were evaluated with the use of dual-energy X-ray absorptiometry. Microhardness of enamel was measured using Vicker's test. Evaluations of total calcium, ionized calcium, magnesium, phosphorus, alkaline phosphatase, bone alkaline phosphatase, osteocalcin, C-terminal telopeptide of type-I collagen (CTX), insulin-like growth factor-1, growth hormone and parathyroid hormone were performed in plasma and serum samples. Pearson's correlation coefficients were determined between all the investigated variables, and P<0.05 was considered as statistically significant. The obtained results have shown mainly mutual dependences between biochemical indicators of bone metabolism. Evaluation of CTX concentration in serum of pigs has shown the highest predictive value in relation to morphological, densitometric and biomechanical properties of teeth. KEY WORDS: bone turnover marker, densitometry, quantitative computed tomography, swine, tooth.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Swine teeth possess mineralization pattern of enamel comparable to that of humans [6]. It is considered that composition of swine enamel is similar to human enamel [2,20]. Experimental studies on skeletal system have also confirmed similarities between bone growth plate structure and mineralization process in humans and pigs [27].…”

mentioning

confidence: 85%

Interrelationships between Tooth Properties and Biochemical Bone Turnover Markers Investigated on Six-Month-Old Pig Model

Tymczyna

Tatara

Krupski

et al. 2013

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

show abstract

“…The specific characteristic of EMD regarding its bone formation ability (osteoinductive, osteoconductive, or osteogenic) was examined by means of a nude mouse muscle implantation assay (Boyan et al, 2000). No ossicle formation occurred when EMD alone was implanted into cell muscle under conditions that support osteoinduction by demineralized freeze-dried bone allograft (DFDBA).…”

Section: (Ii6) In Vivo Studies (Ii61) In Vivo Animal Studiesmentioning

confidence: 99%

“…This study showed that it is possible to induce regeneration of all the periodontal tissues (acellular cementum, periodontal ligament, and alveolar bone) in a way that mimics the normal development of these tissues. In addition, the periodontal regeneration properties of the enamel matrix were associated with the amelogenin fraction .The specific characteristic of EMD regarding its bone formation ability (osteoinductive, osteoconductive, or osteogenic) was examined by means of a nude mouse muscle implantation assay (Boyan et al, 2000). No ossicle formation occurred when EMD alone was implanted into cell muscle under conditions that support osteoinduction by demineralized freeze-dried bone allograft (DFDBA).…”

mentioning

confidence: 99%

The Use of Enamel Matrix Derivative in the Treatment of Periodontal Defects: a Literature Review and Meta-analysis

Venezia

Goldstein

Boyan

et al. 2004

Critical Reviews in Oral Biology & Medicine

Self Cite

158

110

View full text Add to dashboard Cite

Background-Periodontal disease results in the loss of the attachment apparatus. In the last three decades, an increasing effort has been placed on seeking procedures and materials to promote the regeneration of this tissue. The aim of this paper is to evaluate the effect of enamel matrix derivative (EMD) during regenerative procedures. In addition, a meta-analysis is presented regarding the clinical results during regeneration with EMD, to gain evidence as to what can be accomplished following treatment of intrabony defects with EMD in terms of probing depth reduction, clinical attachment level gain, defect fill (using re-entry studies), and radiographic parameters. Methods-The review includes in vitro and in vivo studies as well as human case reports, clinical comparative trials, and histologic findings. In addition, a meta-analysis is presented regarding the regenerative clinical results. For this purpose, we used 28 studies-including 955 intrabony defects treated with EMD that presented baseline and final data on probing depth, clinical attachment level (CAL) gain, or bone gain-to calculate weighted mean changes in the different parameters. The selected studies were pooled from the MEDLINE database at the end of May, 2003. Results-The meta-analysis of intrabony defects treated with EMD resulted in a mean initial probing depth of 7.94 ± 0.05 mm that was reduced to 3.63 ± 0.04 mm (p = 0.000). The mean clinical attachment level changed from 9.4 ± 0.06 mm to 5.82 ± 0.07 mm (p = 0.000). These results were significantly better than the results obtained for either open-flap debridement (OFD) or guided tissue regeneration (GTR). In contrast, histologically, GTR is more predictable than EMD in terms of bone and cementum formation. No advantage was found for combining EMD and GTR. Xenograft, or EMD and xenograft, yielded inferior results compared with EMD alone, but a limited number of studies evaluated this issue. Promising results were noted for the combination of allograft materials and EMD. Conclusions-EMD seems to be safe, was able to regenerate lost periodontal tissues in previously diseased sites based on clinical parameters, and was better than OFD or GTR. Its combination with allograft materials may be of additional benefit but still needs to be further investigated.Key words. Enamel matrix derivative, Emdogain ® , meta-analysis, periodontal regeneration. States Food and Drug Administration (FDA).In 1997, an alternative approach for periodontal regeneration was introduced that was based on embryonic tooth formation (Hammarström, 1997;Heijl et al., 1997). This approach uses an extract of embryonic enamel matrix, termed 'enamel matrix derivative' (EMD), thought to induce mesenchymal cells to mimic the processes that take place during the development of the nascent root and periodontal tissues. The present analysis reviews the data on the effect of EMD as a regenerative promoter. It encompasses in vitro and in vivo studies as well as human case reports, clinical comparative trials, and histologic findin...

show abstract

“…Osteoinductivity was assessed with use of a standardized animal model in which demineralized bone matrix is implanted in the gastrocnemius muscle of nude mice and the presence of new bone is determined by quantitative histomorphometry. This assay has been shown previously to be sensitive enough to assess dose-dependent effects of a variety of additives to demineralized bone matrix, as well as differences in demineralized bone matrix donors and variations between bone banks, and the effects of ovariectomy and estrogen replacement therapy [33][34][35][37][38][39][40][41][42][43][44] . Demineralized bone matrix from three or four pairs of donors from each of four different bone banks was used for the study, enabling us to compare the effect of processing methods used, either terminally irradiated or nonirradiated.…”

mentioning

confidence: 99%

Osteoinductivity of Demineralized Bone Matrix Is Independent of Donor Bisphosphonate Use

Schwartz

Hyzy

Moore

et al. 2011

The Journal of Bone and Joint Surgery-American Volume

View full text Add to dashboard Cite

Porcine Fetal Enamel Matrix Derivative Enhances Bone Formation Induced by Demineralized Freeze Dried Bone Allograft In Vivo

Abstract: EMD is not osteoinductive. However, it is osteopromotive, due in part to its osteoconductive properties, but a threshold concentration is required.

Cited by 161 publications

References 38 publications

Interrelationships between Tooth Properties and Biochemical Bone Turnover Markers Investigated on Six-Month-Old Pig Model

Interrelationships between Tooth Properties and Biochemical Bone Turnover Markers Investigated on Six-Month-Old Pig Model

The Use of Enamel Matrix Derivative in the Treatment of Periodontal Defects: a Literature Review and Meta-analysis

Osteoinductivity of Demineralized Bone Matrix Is Independent of Donor Bisphosphonate Use

Contact Info

Product

Resources

About