Porcine milk exosome miRNAs protect intestinal epithelial cells against deoxynivalenol-induced damage

Xie, Mei-Ying; Chen, Ting; Xi, Qianyun; Hou, Lianjie; Luo, Junyi; Zeng, Bin; Li, Meng; Sun, Jiajie; Zhang, Yongliang

doi:10.1016/j.bcp.2020.113898

Cited by 51 publications

(39 citation statements)

References 45 publications

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“…Milk-derived exosomal miRNAs protect the intestine epithelial cells against LPS-induced damage by inhibiting inflammation and apoptosis ( Xie et al, 2019 ). Our previous study also has demonstrated that milk-derived exosomal miR-181a, miR-365-5p, miR-30c, and miR-769-3p inhibit their targeting genes in the p53 pathway, ultimately attenuating Don-induced intestine damage by promoting proliferation and inhibiting apoptosis ( Xie et al, 2020 ). In this study, our results showed that miR-221/222 inhibit apoptosis and promote proliferation, thereby resisting the Don-induced intestinal injury.…”

Section: Discussionmentioning

confidence: 99%

“…Milk exosome mediates intestinal epithelium cell viability, proliferation, and renewal in the neonatal intestine after birth ( Yu et al, 2017 ). Our previous study has demonstrated that milk exosomal miR-181a, miR-365-5p, miR-30c, and miR-769-3p protect the intestine against Don damage ( Xie et al, 2020 ). Since milk-derived exosome contains many miRNAs, the other milk-derived exosomal miRNAs’ function in regulating intestinal epithelial cells defending against Don-induced injury is still unclear.…”

Section: Introductionmentioning

confidence: 99%

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MiR-221/222 Ameliorates Deoxynivalenol-Induced Apoptosis and Proliferation Inhibition in Intestinal Epithelial Cells by Targeting PTEN

Hou

Tong

Lin

et al. 2021

Front. Cell Dev. Biol.

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Intestinal epithelial cells are critical for nutrient absorption and defending against pathogen infection. Deoxynivalenol (Don), the most common mycotoxin, contaminates cereals and food throughout the world, causes serious damage to mammal intestinal mucosa, and appears as intestinal epithelial cell apoptosis and proliferation inhibition. Our previous study has found that milk-derived exosome ameliorates Don-induced intestinal damage, but the mechanism is still not fully understood. In this study, we demonstrated that Don downregulated the expression of miR-221/222 in intestinal epithelial cells, and exosome treatment reversed the inhibitory effect of Don on miR-221/222. Through immunofluorescence and flow cytometry analysis, we identified that miR-221/222 ameliorates Don-induced apoptosis and proliferation inhibition in intestinal epithelial cells. Through bioinformatics analyses and RNA immunoprecipitation analysis, we identified Phosphatase and tensin homolog (PTEN) is the target of miR-221/222. Through the PTEN interfering experiment, we found Don-induced apoptosis and proliferation inhibition relied on PTEN. Finally, through adenovirus to overexpress miR-221/222 in mice intestinal epithelial cells specifically, our results showed that miR-221/222 ameliorated Don-induced apoptosis and proliferation inhibition in intestinal epithelial cells by targeting PTEN. This study not only expands our understanding of how miR-221/222 and the host gene PTEN regulate intestinal epithelial cells defending against Don-induced damage, but also provides a new way to protect the development of the intestine.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

MiR-221/222 Ameliorates Deoxynivalenol-Induced Apoptosis and Proliferation Inhibition in Intestinal Epithelial Cells by Targeting PTEN

Hou

Tong

Lin

et al. 2021

Front. Cell Dev. Biol.

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“…The fate of unabsorbed exosomes is not yet known. There is strong evidence that milk exosomes are crucial for maturation and intestinal function ( Gao H. N. et al, 2019 ; Gao R. et al, 2019 ; Hock et al, 2017 ; Li et al, 2019 ; Miyake et al, 2020 ; Reif et al, 2019 ; Xie et al, 2020 ). Strong evidence indicates that human, porcine and bovine milk exosomes aid intestinal cell growth in mice ( Gao H. N. et al, 2019 ; Hock et al, 2017 ; Reif et al, 2019 ).…”

Section: Exosomesmentioning

confidence: 99%

Exosomes in Intestinal Inflammation

Ayyar

Moss

2021

Front. Pharmacol.

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Exosomes are 30–150 nm sized vesicles released by a variety of cells, and are found in most physiological compartments (feces, blood, urine, saliva, breast milk). They can contain different cargo, including nucleic acids, proteins and lipids. In Inflammatory Bowel Disease (IBD), a distinct exosome profile can be detected in blood and fecal samples. In addition, circulating exosomes can carry targets on their surface for monoclonal antibodies used as IBD therapy. This review aims to understand the exosome profile in humans and other mammals, the cargo contained in them, the effect of exosomes on the gut, and the application of exosomes in IBD therapy.

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“…In vitro, LPS-induced apoptosis and inflammation of intestinal epithelial cells are decreased by PM-Exs [ 51 ]. The cytoprotective effects are induced by TLR4/NF-κB signaling and apoptotic pathway suppression mediated by exosome microRNAs (miRNAs), suggesting that miRNA-enriched exosomes can be used as a novel preventative strategy for NEC [ 51 , 56 ].…”

Section: Exosomes In Necrotizing Enterocolitis Therapy (Table 3 )mentioning

confidence: 99%

Stem cells and exosomes: promising candidates for necrotizing enterocolitis therapy

et al. 2021

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Necrotizing enterocolitis (NEC) is a devastating disease predominately affecting neonates. Despite therapeutic advances, NEC remains the leading cause of mortality due to gastrointestinal conditions in neonates. Stem cells have been exploited in various diseases, and the application of different types of stem cells in the NEC therapy is explored in the past decade. However, stem cell transplantation possesses several deficiencies, and exosomes are considered potent alternatives. Exosomes, especially those derived from stem cells and breast milk, demonstrate beneficial effects for NEC both in vivo and in vitro and emerge as promising options for clinical practice. In this review, the function and therapeutic effects of stem cells and exosomes for NEC are investigated and summarized, which provide insights for the development and application of novel therapeutic strategies in pediatric diseases. Further elucidation of mechanisms, improvement in preparation, bioengineering, and administration, as well as rigorous clinical trials are warranted.

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Porcine milk exosome miRNAs protect intestinal epithelial cells against deoxynivalenol-induced damage

Cited by 51 publications

References 45 publications

MiR-221/222 Ameliorates Deoxynivalenol-Induced Apoptosis and Proliferation Inhibition in Intestinal Epithelial Cells by Targeting PTEN

MiR-221/222 Ameliorates Deoxynivalenol-Induced Apoptosis and Proliferation Inhibition in Intestinal Epithelial Cells by Targeting PTEN

Exosomes in Intestinal Inflammation

Stem cells and exosomes: promising candidates for necrotizing enterocolitis therapy

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