Porcine Small Intestinal Submucosa Mitral Valve Material Responses Support Acute Somatic Growth

Gonzalez, Brittany A.; Issa, Elnaz Pour; Mankame, Omkar; Bustillos, Jenniffer; Cuellar, Antonio Valencia; Rodriguez, Antonio B.; Scholl, Frank G.; Bibevski, Steven; Hernandez, Lazaro E.; Brehier, Vincent; Casares, Mike; Rivas-Wagner, Krishna; Morales, Pablo; López, Jesús M.; Wagner, Joseph; Bibevski, Jennifer; Agarwal, Arvind; George, Florence; Ramaswamy, Sharan

doi:10.1089/ten.tea.2019.0220

Cited by 8 publications

(7 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It gives SIS material good mechanical strength. Currently, SIS has been used in repair and reconstruction surgery [13][14][15][16]. The use of SIS materials in the urinary systems has also been reported [2][3][4]16].…”

Section: Discussionmentioning

confidence: 99%

Using of Single‐Layer Porcine Small Intestinal Submucosa in Urethroplasty on a Beagle Model

Qin

Wang

et al. 2022

BioMed Research International

View full text Add to dashboard Cite

Purpose. To evaluate the role of porcine small intestinal submucosa (SIS) in reducing fistula during urethroplasty and to observe its degradation process in beagle models. Methods. 22 male beagles were divided into the SIS group and control group. All animals received surgical operation to establish the hypospadias model. Urethroplasty was followed. In the SIS group, the urethra was covered with a single-layer SIS material while no SIS material covered in the control group. At the 2nd, 4th, and 12th weeks after the operation, there were 3, 3, and 5 animals in each group, respectively, sacrificed for surgical site histological examinations. The inflammation reaction and collagen hyperplasia levels were assessed. The fistula was identified by retrograde cystourethrography at the 4th and 12th weeks after the operation. Results. the incidence of urethral fistula was 25% (2/8) in the SIS group and 75% (6/8) in the control group. The inflammation reaction of SIS and control groups had no significant difference ( U = 52.50 , P = 0.58 ). The collagen fiber increased in both groups; however, the SIS group had a much more gentle increase compared to the control group ( U = − 0.00 , P < 0.001 ). In the SIS group, the SIS material was roughly complete on the specimens 2 w after surgery but became loose and discontinuous 4 w after surgery and could not be found 12 w after surgery. Conclusion. The material can decrease the incidence of urethral fistula in the animal models, when used as a coverage layer. The SIS degradation process started 2 w–4 w after the operation and finished before 12 w in the animal model.

show abstract

Section: Discussionmentioning

confidence: 99%

Using of Single‐Layer Porcine Small Intestinal Submucosa in Urethroplasty on a Beagle Model

Qin

Wang

et al. 2022

BioMed Research International

View full text Add to dashboard Cite

show abstract

“…Despite this work is not the first to employ a bioreactor system to assess cell viability inside a decellularized scaffold for cardiac valve engineering (33,34), it is the first to address the problem of valve maturation of a fully recellularized tissue using a systematic proteomic approach. In fact, our mass spectrometry data clearly showed that compared to the native pericardial membrane, a number of proteins were upregulated in the material as a result of de-novo protein synthesis by the introduced valve cells.…”

Section: Discussionmentioning

confidence: 99%

Culture Into Perfusion-Assisted Bioreactor Promotes Valve-Like Tissue Maturation of Recellularized Pericardial Membrane

Amadeo

Barbuto

Bernava

et al. 2020

Front. Cardiovasc. Med.

View full text Add to dashboard Cite

Derivation of tissue-engineered valve replacements is a strategy to overcome the limitations of the current valve prostheses, mechanical, or biological. In an effort to set living pericardial material for aortic valve reconstruction, we have previously assessed the efficiency of a recellularization strategy based on a perfusion system enabling mass transport and homogenous distribution of aortic valve-derived "interstitial" cells inside decellularized pericardial material. In the present report, we show that alternate perfusion promoted a rapid growth of valve cells inside the pericardial material and the activity of a proliferation-supporting pathway, likely controlled by the YAP transcription factor, a crucial component of the Hippo-dependent signaling cascade, especially between 3 and 14 days of culture. Quantitative mass spectrometry analysis of protein content in the tissue constructs showed deposition of valve proteins in the decellularized pericardium with a high variability at day 14 and a reproducible tissue maturation at 21 days. These results represent a step forward in the definition of strategies to produce a fully engineered tissue for replacing the calcified leaflets of failing aortic valves.

show abstract

“…Biomechanics mainly comprises force transmission and transduction among ECM, cell adhesion, and cytoskeleton [114][115][116][117] within cardiac tissues. Biomechanics are associated with cardiac tissue configuration, micro-/macrostructure, and functions.…”

Section: Mechanical Properties Of Soft Biomaterials For Cardiac Tissu...mentioning

confidence: 99%

Integrated Perspective of Scaffold Designing and Multiscale Mechanics in Cardiac Bioengineering

Lou

Lopez

Nautiyal

et al. 2021

Advanced NanoBiomed Research

Self Cite

View full text Add to dashboard Cite

According to a World Health Organization (WHO) report in 2017, [1] cardiovascular diseases (CVDs) are the foremost cause of mortality, with 85% attributed to stroke and heart attack (or myocardial infarction [MI]) worldwide. The 2019 American Heart Association report [2] indicates that CVD is the leading lethal disease in the U.S., with 840 768 deaths in 2016. There is one new MI case in the U.S. every 40 s, contributing to about $351 billion in costs in 2014À2015. [2] MI gradually develops and deteriorates into inflammation, apoptosis, and inelastic fibrous scar, eventually leading to congestive heart failure (CHF). [3] Due to cardiomyocytes' (CMs) limited regeneration and proliferation ability, the effective long-term MI and CHF treatment is transplantation. There were over 3500 candidates on the heart transplant waiting list by the end of 2020, according to the national data [4] from the USA. Department of Health and Human Services. Shortage of organ donation sources, organ storage and maintenance, and post-transplantation immunologic rejection are tricky issues that are difficult to solve. Reconstruction of cardiac tissue using 3D scaffold or scaffold-free techniques has emerged as a promising alternative treatment approach.Cardiac bioengineering aims to investigate or rebuild illfunctioning myocardium tissues via CMs, scaffold-free cell sheet substrates, and implantable cell-seeded scaffolds. [3] Scaffold-free approaches include miRNA clusters, [5] spheroid models, [6] scaffold-free multiple-component cell cultures, [3,7] injectable hydrogels, [8] and organ on a chip. [9] These strategies permit direct delivery of cells or cell composites to infarcted myocardium and enable thick 3D tissue patch implantation with enhanced cell engraftment capacity. Some scaffold-free approaches can also be exploited in in vitro pathological cardiac models or ex vivo platforms for new therapy efficacy and cardiotoxicity evaluation. [6,7,9] Unlike scaffold-free pathways, cardiac scaffolds provide mechanical support to the myocardium, establish contractility/pumping, and lessen myocardial dilation and myocardial wall stress. [3] Meanwhile, significant progress has been made in stem cellderived CM tissue preparation; [10][11][12] scaffold and extracellular matrix (ECM) construction; [13][14][15][16][17] scaffold-free in situ injectable pathway development; [5][6][7]9] cell seeding, proliferation, and viabil-

show abstract

Porcine Small Intestinal Submucosa Mitral Valve Material Responses Support Acute Somatic Growth

Cited by 8 publications

References 59 publications

Using of Single‐Layer Porcine Small Intestinal Submucosa in Urethroplasty on a Beagle Model

Using of Single‐Layer Porcine Small Intestinal Submucosa in Urethroplasty on a Beagle Model

Culture Into Perfusion-Assisted Bioreactor Promotes Valve-Like Tissue Maturation of Recellularized Pericardial Membrane

Integrated Perspective of Scaffold Designing and Multiscale Mechanics in Cardiac Bioengineering

Contact Info

Product

Resources

About