Pore formation in lipid membrane II: Energy landscape under external stress

Akimov, Sergey A.; Volynsky, Pavel E.; Galimzyanov, Timur R.; Kuzmin, Peter I.; Павлов, Константин; Batishchev, Oleg V.

doi:10.1038/s41598-017-12749-x

Cited by 88 publications

(95 citation statements)

References 46 publications

(110 reference statements)

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“…The difference between DC‐glycerol and DC‐MTS can be explained by considering the lipid molecules as flexible chains and the intermolecular bonds due to reticulation as consolidating structures. In DC‐glycerol‐R, the upper part (above the reticulation bonds) of the lipid layer can splay under the electric field, hence creating opened and disorganized regions with reduced insulating performances. It was indeed shown that for self‐assembled monolayers of long alkyl chains the leakage current density varies drastically with the chain length (for small chain length it is mainly due to electron tunneling through the layer) as well as with the compacity and ordering of the layer .…”

Section: Resultssupporting

confidence: 77%

“…Both mechanisms have been studied theoretically in water for nonreticulated supported lipid layers or cell membranes . In the framework of nanoindentation experiments, Butt and co‐workers developed a two‐state film rupture reaction theory based on the assumption that an AFM tip can rupture the lipid layer after the formation of a sufficiently large hole under the tip.…”

Section: Resultsmentioning

confidence: 99%

“…The lipid layer can be considered as a planar capacitor, which tends to increase its capacitance in order to minimize electric energy. The electric field pushes water molecules into the membrane to substitute the lipids with low dielectric constant, by water with high dielectric constant, thus creating an effective lateral pressure applied to the pore edge . The result of this mechanism leads to a compression of the lipid layer, i.e., to an electrostatic pressure, which leads to the opening of a hole, similar to the case of mechanoporation.…”

Section: Resultsmentioning

confidence: 99%

“…Drying off or exposing the sample to solvents usually induces lipid layer destruction, therefore limiting its use to applications in solution as well as its storage. Several strategies have been carried out in order to overcome these issues including the direct substrate surface bonding or the internal crosslinking within the plane of the lipid layer . Interestingly, it was previously shown that the mechanical force required to disrupt a self‐assembled monolayer made from 1,2‐bis‐(10,12‐tricosadiynoyl)‐sn‐glycero‐3‐phosphocholine (DC8, 9 PC, or DC‐PC) (SLM, supported lipid monolayer) can be aptly improved, by a factor of ten, after a radical reticulation within the plane of the monolayer .…”

Section: Introductionmentioning

confidence: 99%

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Ultrathin Supported Lipid Monolayer with Unprecedented Mechanical and Dielectric Properties

Kenaan

Zein

Kilinc

et al. 2018

Adv Funct Materials

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The development of ultrathin dielectrics for low power electronics operations, flexible and printed electronics, and field‐effect‐transistor‐based sensors is still a challenge. Here, monolayers of engineered lipids supported on silicon are reported presenting exceptional mechanical and dielectric properties. The lipid monolayers are stabilized using a simple procedure based on a two‐stage reticulation process in both their aliphatic chains and their head‐group. With a thickness lower than 3 nm, such layers are demonstrated to offer exceptional mechanical and dielectric stability with unprecedented low leakage current and dielectric strength. Surprisingly, the mechanical and dielectric pressures required to rupture/breakdown the monolayers are shown to be similar. These results suggest the presence of a strong correlation between mechanical and dielectric properties, as well as between the mechanisms of rupture and breakdown.

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Section: Resultssupporting

confidence: 77%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Ultrathin Supported Lipid Monolayer with Unprecedented Mechanical and Dielectric Properties

Kenaan

Zein

Kilinc

et al. 2018

Adv Funct Materials

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“…To explain the mechanism of the line activity, a concept of so-called hybrid lipids was proposed 28 : in order to be line-active, hybrid lipids www.nature.com/scientificreports www.nature.com/scientificreports/ calculated up to the second order in deformations, which are assumed to be small. In a quadratic approximation, the elastic energy of the monolayer can be expressed as 40,45,46 : Scientific RepoRtS | (2020) 10:4087 | https://doi.…”

mentioning

confidence: 99%

Elastic deformations mediate interaction of the raft boundary with membrane inclusions leading to their effective lateral sorting

Pinigin

Kondrashov

Jiménez-Munguía

et al. 2020

Sci Rep

Self Cite

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Liquid-ordered lipid domains represent a lateral inhomogeneity in cellular membranes. these domains have elastic and physicochemical properties different from those of the surrounding membrane. in particular, their thickness exceeds that of the disordered membrane. thus, elastic deformations arise at the domain boundary in order to compensate for the thickness mismatch. in equilibrium, the deformations lead to an incomplete register of monolayer ordered domains: the elastic energy is minimal if domains in opposing monolayers lie on the top of each other, and their boundaries are laterally shifted by about 3 nm. This configuration introduces a region, composed of one ordered and one disordered monolayers, with an intermediate bilayer thickness. Besides, a jump in a local monolayer curvature takes place in this intermediate region, concentrating here most of the elastic stress. This region can participate in a lateral sorting of membrane inclusions by offering them an optimal bilayer thickness and local curvature conditions. In the present study, we consider the sorting of deformable lipid inclusions, undeformable peripheral and deeply incorporated peptide inclusions, and undeformable transmembrane inclusions of different molecular geometry. With rare exceptions, all types of inclusions have an affinity to the ordered domain boundary as compared to the bulk phases. the optimal lateral distribution of inclusions allows relaxing the elastic stress at the boundary of domains.Cellular membranes are laterally heterogeneous 1-3 . Many membrane proteins are believed to function properly only inside lipid-protein domains, also referred to as rafts 4-7 . Proteins in these domains are surrounded by a more or less thick lipid shell [8][9][10][11] . In model purely lipidic systems it is demonstrated that lipids can form similar domains, in which they are in a liquid-ordered (L o ) phase state, while the surrounding membrane is liquid-disordered (L d ) [12][13][14][15] . Ordered domains are usually bilayer, i.e. exist in both membrane leaflets at the same lateral position [14][15][16][17] . Such transbilayer coupling could be driven by elastic deformations arising at the domain boundary and by membrane thermal fluctuations 18-21 . The mechanism of this coupling is not specific to the exact lipid composition of the domain: only the higher ordering of lipids with respect to the surrounding membrane is important [22][23][24] . A bilayer structure of rafts is believed to be a key property in providing raft-dependent signal transduction across the plasma membrane 4,5 . There are increasing evidences that a raft interior itself may be laterally inhomogeneous: some molecules may prefer its boundary region rather than its bulk part. In particular, the fusion peptide of the human immunodeficiency virus (HIV) gp41 protein functions with the highest efficiency only in the presence of the L o /L d phase boundary in the target membrane 25,26 . In addition, the HIV receptor CCR5 preferentially localizes at the domain boundary 26 . It means t...

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A Comprehensive Review on Intracellular Delivery

Rad

Bazaz

et al. 2021

Advanced Materials

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Intracellular delivery is considered an indispensable process for various studies, ranging from medical applications (cell‐based therapy) to fundamental (genome‐editing) and industrial (biomanufacture) approaches. Conventional macroscale delivery systems critically suffer from such issues as low cell viability, cytotoxicity, and inconsistent material delivery, which have opened up an interest in the development of more efficient intracellular delivery systems. In line with the advances in microfluidics and nanotechnology, intracellular delivery based on micro‐ and nanoengineered platforms has progressed rapidly and held great promises owing to their unique features. These approaches have been advanced to introduce a smorgasbord of diverse cargoes into various cell types with the maximum efficiency and the highest precision. This review differentiates macro‐, micro‐, and nanoengineered approaches for intracellular delivery. The macroengineered delivery platforms are first summarized and then each method is categorized based on whether it employs a carrier‐ or membrane‐disruption‐mediated mechanism to load cargoes inside the cells. Second, particular emphasis is placed on the micro‐ and nanoengineered advances in the delivery of biomolecules inside the cells. Furthermore, the applications and challenges of the established and emerging delivery approaches are summarized. The topic is concluded by evaluating the future perspective of intracellular delivery toward the micro‐ and nanoengineered approaches.

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Pore formation in lipid membrane II: Energy landscape under external stress

Cited by 88 publications

References 46 publications

Ultrathin Supported Lipid Monolayer with Unprecedented Mechanical and Dielectric Properties

Ultrathin Supported Lipid Monolayer with Unprecedented Mechanical and Dielectric Properties

Elastic deformations mediate interaction of the raft boundary with membrane inclusions leading to their effective lateral sorting

A Comprehensive Review on Intracellular Delivery

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