Pore size of macroporous polystyrene microspheres affects lipase immobilization

Li, Yan; Gao, Fei; Wei, Wei; Qu, Jian‐Bo; Ma, Guanghui; Zhou, Weiqing

doi:10.1016/j.molcatb.2010.05.007

Cited by 148 publications

(86 citation statements)

References 27 publications

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“…4D). This modified xylan-PVOH bead could be categorized as a macroporous material, but the PVOH bead could be classified as a gigaporous material that can provide more efficient cell or compound entrapment (microporous materials (less than 0.002 μm), mesoporous materials (0.002 to 0.050 μm), macroporous materials (0.05 to 0.20 μm), and gigaporous materials (more than 0.2 μm)) (Li et al 2010). Pore size plays an important role in enzyme immobilization.…”

Section: Morphology Of the Modified Xylan-pvoh Beadsmentioning

confidence: 99%

“…Pore size plays an important role in enzyme immobilization. Although several studies of enzyme immobilization have mainly focused on the micro-or mesoporous materials such as silica SBA-15 (Yang et al 2013), chitosan with epichlorohydrin (Bayramoglu et al 2012), and cellulose acetate (Güleç 2013), there are usually constraints of mass transfer and diffusion of the enzyme (Li et al 2010;Jesionowski et al 2014). With a small pore size carrier, the enzyme can primarily be immobilized on the surface, and it is not well protected by the carrier, leading to the remarkable decrease in activity recovery.…”

Section: Morphology Of the Modified Xylan-pvoh Beadsmentioning

confidence: 99%

See 1 more Smart Citation

A Novel Xylan-Polyvinyl Alcohol Hydrogel Bead with Laccase Entrapment for Decolorization of Reactive Black 5

Bankeeree¹,

Prasongsuk²,

Imai³

et al. 2016

BioResources

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In an attempt to find a more efficient technique for biodegradation of the recalcitrant Reactive Black 5 (RB-5) dye, a composite xylan-polyvinyl alcohol (xylan-PVOH) hydrogel was used to immobilize laccase from the white-rot fungus Trametes versicolor. Xylan was prepared from the black liquor of pulp and paper effluent, and it was esterified with citric acid prior to cross-linking with polyvinyl alcohol (PVOH). The optimum composition for the immobilized laccase bead formation consisted of 4% (w/v) modified xylan, 10% (w/v) PVOH, and 15 U.mL -1 crude laccase. The maximum decolorization of RB-5 (98.45  1.96 %) was obtained within the first cycle (6 h) at 40 °C. In the eighth cycle, the reused beads were able to decolorize 55.35  2.46 % of the RB-5. Moreover, the xylan-PVOH beads extended the optimum pH range of laccase activity from 6 to 10 and tolerated a temperature up to 10 °C higher than that of the free enzyme. These results suggest that the xylan-PVOH bead has great potential as the polymer matrix for enzyme immobilization, which has applications in wastewater treatment.

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Section: Morphology Of the Modified Xylan-pvoh Beadsmentioning

confidence: 99%

Section: Morphology Of the Modified Xylan-pvoh Beadsmentioning

confidence: 99%

A Novel Xylan-Polyvinyl Alcohol Hydrogel Bead with Laccase Entrapment for Decolorization of Reactive Black 5

Bankeeree¹,

Prasongsuk²,

Imai³

et al. 2016

BioResources

View full text Add to dashboard Cite

show abstract

“…Li et al investigated how the pore size affects the lipase distribution, thermal stability, storage stability, and reusability. 55 Their results showed that the thermal stability, storage stability, and reusability were all improved significantly with increased Higher proliferation in larger pore sizes (500 µm).…”

Section: Carriers For Biomacromolecules or Other Substancesmentioning

confidence: 99%

“…[52][53][54][55][56][57][58][59][60][61] High-speed protein chromatography Due to the huge specific surface area, porous microspheres are fit for adsorption and desorption substances. Thus a separation of substances can be realized.…”

Section: Applications Of Porous Microspheres Tissue Regeneration Scafmentioning

confidence: 99%

Porous microsphere and its applications

Cai¹,

Chen²,

Hong³

et al. 2013

IJN

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Porous microspheres have drawn great attention in the last two decades for their potential applications in many fields, such as carriers for drugs, absorption and desorption of substances, pulmonary drug delivery, and tissue regeneration. The application of porous microspheres has become a feasible way to address existing problems. In this essay, we give a brief introduction of the porous microsphere, its characteristics, preparation methods, applications, and a brief summary of existing problems and research tendencies.

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“…Enzymes immobilization onto carriers has been extensively studied and applied in many fields, such as biocatalysts (Hou et al, 2007;Li et al, 2010) medical devices (Liang et al, 2000;Lao et al, 2008), drug delivery systems (Gan and Wang, 2007;Shi et al, 2011a) and biosensor (Ley et al, 2011;Samanta and Sarkar, 2011). Several carriers such as synthetic organic polymers (e.g., Eupergit C and polyurethane), biopolymers (e.g., alginate), hydrogels (e.g., Polyvinyl alcohol), smart polymers (e.g., poly-N-isopropylacrylamide) and inorganic supports (e.g., alumina, silica and zeolites) have been used in immobilization of enzymes (Katchalski-Katzir and Kraemer, 2000;Kirk and Christensen, 2002;Temino et al, 2005;Lutz et al, 2006;Awang et al, 2007;Sheldon, 2007).…”

Section: Immobilized Enzyme Supportsmentioning

confidence: 99%

Potential Applications of Chitosan Nanoparticles as Novel Support in Enzyme Immobilization

Malmiri

Jahanian

Berenjian

2012

American Journal of Biochemistry and Biotechnology

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Chitosan is an attractive natural biopolymer from renewable resources with the presence of reactive amino and hydroxyl functional groups in its structure. Due to the good biocompatibility of chitosan, it can be used in magnetic-field assisted drug delivery, enzyme or cell immobilization and many other industrial applications. In the past decade, nanotechnology has been a considerable research interest in the area of preparation of immobilized enzyme carriers. This study looks at characteristics and applications of chitosan and chitosan nanoparticles and their potentials as suitable supports for enzyme immobilization. Results indicated that activity of immobilized enzymes and performance of enzyme immobilization onto chitosan nanoparticles are higher than chitosan macro and microparticles. As compared to other biopolymers nanoparticles, application of chitosan nanoparticles to immobilize enzymes strongly increases stability of immobilized enzymes and their easy separability from the reaction mixture at the end of the biochemical process.

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Pore size of macroporous polystyrene microspheres affects lipase immobilization

Cited by 148 publications

References 27 publications

A Novel Xylan-Polyvinyl Alcohol Hydrogel Bead with Laccase Entrapment for Decolorization of Reactive Black 5

A Novel Xylan-Polyvinyl Alcohol Hydrogel Bead with Laccase Entrapment for Decolorization of Reactive Black 5

Porous microsphere and its applications

Potential Applications of Chitosan Nanoparticles as Novel Support in Enzyme Immobilization

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