Porous chitosan scaffold containing microspheres loaded with transforming growth factor-β1: Implications for cartilage tissue engineering

Kim, Sung Eun; Park, Jae Hyung; Cho, Yong Woo; Chung, Heesun; Jeong, Seo Young; Lee, Eunhee Bae; Kwon, Ick Chan

doi:10.1016/s0168-3659(03)00274-8

Cited by 272 publications

(153 citation statements)

References 41 publications

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“…However, the tests we employed are commonly used and give sufficient evidence that osteogenesis occurred and to what extent. Indeed, our encapsulation efficiency for BMP-2 is lower than some other values reported in the literature for GFs [ 9,24,34]. However, many of the reported encapsulation methods include the use of high temperatures and considerable amounts of toxic organic solvents without using a carrier protein to protect the GFs.…”

Section: Discussionmentioning

confidence: 59%

Starch-poly-є-caprolactone Microparticles Reduce the Needed Amount of BMP-2

Balmayor

Feichtinger

Azevedo

et al. 2009

Clin Orthop Relat Res

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BMP-2 is currently administered clinically using collagen matrices often requiring large amounts of BMP-2 due to burst release over a short period of time. We developed and tested a novel injectable drug delivery system consisting of starch-poly-e-caprolactone microparticles for inducing osteogenesis and requiring smaller amounts of BMP-2. We evaluated BMP-2 encapsulation efficiency and the in vitro release profile by enzyme-linked immunosorbent assay. BMP-2 was rapidly released during the first 12 hours, followed by sustained release for up to 10 days. We then evaluated the osteogenic potential of dexamethasone (standard osteogenic induction agent) and BMP-2 after incorporation and during release using an osteo/myoblast cell line (C2C12). Alkaline phosphatase activity was increased by released BMP-2. Mineralization occurred after stimulation with BMP-2-loaded microparticles. A luciferase assay for osteocalcin promoter activity showed high levels of activity upon treatment with BMP-2-loaded microparticles. In contrast, no osteogenesis occurred in C2C12 cells using dexamethasone-loaded microparticles. However, human adipose stem cells exposed to the microparticles produced high amounts of alkaline phosphatase. The data suggest starch-poly-e-caprolactone microparticles are suitable carriers for the incorporation and controlled release of glucocorticoids and growth factors. Specifically, they reduce the amount of BMP-2 needed and allow more sustained osteogenic effects.

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Section: Discussionmentioning

confidence: 59%

Starch-poly-є-caprolactone Microparticles Reduce the Needed Amount of BMP-2

Balmayor

Feichtinger

Azevedo

et al. 2009

Clin Orthop Relat Res

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“…Chitosan dissolved in acetic acid was ejected and electrospun to the grounded Na 2 CO 3 solution for neutralization of chitosan. Chitosan, a positively-charged natural polymer, was solubilized in acetic acid and the acetate ion was bound to the protonated amine groups by electrostatic interactions (30,31). For solidification of CMs, the acetate groups need to be scavenged at high pH, where the primary amine groups of the chitosan are deprotonated.…”

Section: Discussionmentioning

confidence: 99%

Electrospun Chitosan Microspheres for Complete Encapsulation of Anionic Proteins: Controlling Particle Size and Encapsulation Efficiency

et al. 2013

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Abstract. Electrospinning was employed to fabricate chitosan microspheres by a single-step encapsulation of proteins without organic solvents. Chitosan in acetic acid was electrospun toward a grounded sodium carbonate solution at various electric potential and feeding rates. Electrospun microspheres became insoluble and solidified in the sodium carbonate solution by neutralization of chitosan acetate. When the freeze-dried microspheres were examined by scanning electron microscopy, the small particle size was obtained at higher voltages. This is explained by the chitosan droplet size at the electrospinning needle was clearly controllable by the electric potential. The recovery yield of chitosan microspheres was dependent on the concentration of chitosan solution due to the viscosity is the major factor affecting formation of chitosan droplet during curling of the electrospinning jets. For protein encapsulation, fluorescently labeled bovine serum albumin (BSA) was codissolved with chitosan in the solution and electrospun. At higher concentration of sodium carbonate solution and longer solidification time in the solution, the encapsulation efficiency of the protein was confirmed to be significantly high. The high encapsulation efficiency was achievable by instant solidification of microspheres and electrostatic interactions between chitosan and BSA. Release profiles of BSA from the microspheres showed that the protein release was faster in acidic solution due to dissolution of chitosan. Reversed-phase chromatography of the released fractions confirmed that exposure of BSA to acidic solution during the electrospinning did not result in structural changes of the encapsulated protein.

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“…Therefore, chitosan has been used as a good compatible material for tissue repair and wound healing [11,[23][24][25]. In cartilage tissue engineering, previous studies have shown that this material possesses promising potential as a carrier material for the transplant of chondrocytes [12,[26][27][28][29][30].…”

Section: Chitosan-based Ha Hybrid Polymer Fiber Preparationmentioning

confidence: 99%

Chitosan-Based Hyaluronic Acid Hybrid Polymer Fibers as a Scaffold Biomaterial for Cartilage Tissue Engineering

et al. 2010

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An ideal scaffold material is one that closely mimics the natural environment in the tissue-specific extracellular matrix (ECM). Therefore, we have applied hyaluronic acid (HA), which is a main component of the cartilage ECM, to chitosan as a fundamental material for cartilage regeneration. To mimic the structural environment of cartilage ECM, the fundamental structure of a scaffold should be a three-dimensional (3D) system with adequate mechanical strength. We structurally developed novel polymer chitosan-based HA hybrid fibers as a biomaterial to easily fabricate 3D scaffolds. This review presents the potential of a 3D fabricated scaffold based on these novel hybrid polymer fibers for cartilage tissue engineering.

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Porous chitosan scaffold containing microspheres loaded with transforming growth factor-β1: Implications for cartilage tissue engineering

Cited by 272 publications

References 41 publications

Starch-poly-є-caprolactone Microparticles Reduce the Needed Amount of BMP-2

Starch-poly-є-caprolactone Microparticles Reduce the Needed Amount of BMP-2

Electrospun Chitosan Microspheres for Complete Encapsulation of Anionic Proteins: Controlling Particle Size and Encapsulation Efficiency

Chitosan-Based Hyaluronic Acid Hybrid Polymer Fibers as a Scaffold Biomaterial for Cartilage Tissue Engineering

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