Hydrogel is a very promising dressing for hemostasis and wound healing due to its good adhesion and long‐term moist environment. However, it can't be ignored that the secondary injury caused by tissue adhesion due to homogeneous hydrogel. The obvious interface exist in Janus hydrogel will weaken its asymmetric function. Here, a hierarchical adhesive polyacrylic acid‐polyurushiol water‐oil Janus hydrogel (JPs@PAA‐PU) without adhesive layer was fabricated by one‐pot method in the stabilization of polystyrene@silica‐siliver (JPs) Janus particles. The morphological structure, mechanical properties, anisotropic chemical composition and adhesion performance, in vivo and in vitro hemostatic properties of Janus hydrogel were investigated. Result showed that the obtained Janus hydrogel possessed obvious compartmentalization in microstructure, functional groups and chemical elements. Janus hydrogel was provided with asymmetric interfacial toughness with top 52.45±2.29 Kpa and bottom 7.04±0.88 Kpa on porcine liver. The adhesion properties of PAA side to tissue, RBCs and platelets, the promoting effect of PU side on coagulation cascade reaction and its physical battier endow Janus hydrogel with shorter hemostatic time and less blood loss than control group. It also exhibited excellent antibacterial effects against E. coli and S. aureus (>90%). Janus hydrogel possessed biosafety, which provided safety guarantee for clinical applications in the future.This article is protected by copyright. All rights reserved