Porous gelatin–siloxane hybrid scaffolds with biomimetic structure and properties for bone tissue regeneration

Abstract

We produced highly porous gelatin-siloxane (GLA-S) hybrid scaffolds with biomimetic anisotropic porous structure, physiochemical properties, mechanical behaviors and biological functions by treating gelatin-siloxane hybrid gels in an ammonium hydroxide solution. The siloxane used as an inorganic phase could effectively crosslink the gelatin polymer, which allowed for the unidirectional enlargement of ammonia vacuoles during ammonium hydroxide treatment. This created aligned pores in an axial direction when the siloxane contents (10 and 20 wt %) were high. In addition, the gelatin polymer could be uniformly hybridized with the siloxane phase at the molecular level, while intense interaction between these two phases could be achieved. This resulted in a significant increase in mechanical properties. The GLA-S hybrid scaffold with a siloxane content of 10 wt % showed reasonably high compressive yield strength of 4.2 ± 0.1 MPa and compressive modulus of 84 ± 5 MPa at a porosity of 86 vol %, which would be comparable to those of natural cancellous bone. In addition, the GLA-S hybrid scaffold had good biocompatibility assessed by in vitro cell tests using pre-osteoblast MC3T3-E1 cells.

Concentrated gelatin/alginate composites for fabrication of predesigned scaffolds with a favorable cell response by 3D plotting

“…[52][53][54] In this study, we demonstrate that a high concentration gelatin/alginate composite is similarly suitable to support cell adhesion and proliferation (Fig. [52][53][54] In this study, we demonstrate that a high concentration gelatin/alginate composite is similarly suitable to support cell adhesion and proliferation (Fig.…”

Content-dependent biomineralization activity and mechanical properties based on polydimethylsiloxane–bioactive glass–poly(caprolactone) hybrids monoliths for bone tissue regeneration

“…13 To solve these problems, many BG nanoparticles (BGN) reinforced polymers including poly(caprolactone) (PCL) and gelatin were fabricated and proposed as potential biomaterials for bone tissue regeneration. [20][21][22] Generally, hydrophilic gelatin and chitosan was easily to hybridize with BG sol to form the hybrid structure. However, BGN-based polymer systems usually possess unstable mechanical properties and non-uniform biomineralization activity, due to the poor size distribution and low interface interactions between inorganic and polymer phase.…”

Immobilization of polyvinyl alcohol‐siloxane on the oxygen plasma‐modified polyurethane‐carbon nanotube composite matrix

“…The observed results (FE-SEM images and EDX spectrum) confirmed this phenomenon. [74][75][76] Moreover, a variety of studies reveals that negative surface charges of CNT can electrostatically combine with positively charged SBF ions. Herein, the coating layer restricted easy access of CNTs to SBF ions so the potential of HAP mineralization can reduce.…”