Porous HA/Alumina composites intended for bone-tissue engineering

Abstract

Ceramic biomaterials based on hydroxyapatite (HA) or alumina have been intensively studied due to their load-bearing applications in the bone-tissue replacement/reconstruction and dental applications. Here we present a study of the preparation and properties of HA/alumina (HA/Al) composites with a targeted porosity. The HA powder used for the composite's preparation was synthetized via a precipitation method under a variety of pH values. The resulting powders were verified with XRD, Raman and FTIR analyses. The particle size was assessed via SEM and laser diffraction. The as-prepared HA nanopowder and alumina powder (median 3 μm) were homogenously mixed having a composition of HA/Alumina = 90/10 (w/w). A suspension with 65 % mass fraction of the powders was properly mixed and, with the help of foaming agents, it was foamed in situ. The behavior under an increasing temperature was studied, using a heating microscope and dried foams were sintered under determined temperatures. The final sintered foams were examined in vitro in a synthetic body fluid, which predicted the behavior of bone implants in vivo. The behavior of the treated samples was studied with SEM. The newly formed HA composites were confronted with Ca 2+ and PO4 3-contents in the applied body-fluid solution.Keywords: hydroxyapatite/Al2O3 composite, ceramic scaffolds, in-vitro bioactivity, SEM Kerami~ni biomateriali na osnovi hidroksiapatitov (HA) ali aluminijevega oksida so bili sistematsko preiskovani zaradi njihove najbolj{e nadomestne vloge pri nadome{~anju kostnega tkiva/rekonstrukciji in pri zobnih vsadkih. Predstavljena je {tudija priprave in lastnosti kompozitov HA / aluminijevega oksida (HA / Al) s ciljem preizkusa poroznosti. HA prah, ki se uporablja za pripravo kompozitov, smo sintetizirali po postopku obarjanja pri razli~nih pH-vrednostih. Dobljene pra{ke smo preverili z XRD-, Raman in FTIR-analizo. Velikost delcev je bila ocenjena s pomo~jo SEM in z lasersko difrakcijo. Pripravljen nanopra{ek (HA) in aluminijev oksid v prahu (median 3 μm) sta bila homogeno preme{ana v me{anico HA/aliminij 90/10 (w/w). Suspenzija s 65 masnimi % pra{kov je bila ustrezno me{ana in s pomo~jo penil spenjena in situ. Raziskovali smo obna{anje v okviru nara{~ajo~e temperature, uporabo pod ogrevanim mikroskopom in suhe pene so bile sintrane pri dolo~enih temperaturah. Kon~ne sintrane pene so bile pregledane in vitro kot sinteti~ne teko~ine, ki so napovedale obna{anje kostnih vsadkov in vivo. Obna{anje obdelanih vzorcev smo preu~evali s SEM. Pri vstavitvi oz. stiku s telesno teko~ino so bili na novo oblikovani HA v stiku z Ca 2+ in vsebino PO4 3-.Klju~ne besede: hidroksiapatit/AI2O3 kompozit, kerami~na ogrodja, bioaktivnost in vitro, SEM

In-Vitro Bioactivity Investigation of Sol-Gel Derived Alumina-Bovine Hydroxyapatite (BHA) Composite Powders

“…TCP and tetracalcium phosphate (TTCP) formed after the decomposition of oxyapatite but TTCP did not appear in the presented composite samples [28,29]. It is interpreted that the decomposition process of HA was partially completed so that HA and TCP phases were identified together in the structure instead of TTCP and TCP [6]. As it is well known from the literature, α-Al2O3, HA and TCP phases take place in the bioceramics class and are intensely used in biomedical applications in terms of prostheses and implants.…”

“…It is possible to encounter with studies and reports based on synthesis of Al2O3-HA composites and their properties [6][7][8][9][10][11]. Sol-gel process is ideal to produce high chemical purity powders.…”

Comprehensive Survey on Nanobiomaterials for Bone Tissue Engineering Applications

“…were used to synthesize different particle sized porous alumina ceramics [ 401 ]. Hydroxyapatite/alumina composite based foam was synthesized via a precipitation method under a variety of pH values that showed a good concentration of Ca 2+ and PO 4 3− contents [ 402 ]. The chemical modification of porous alumina surface with vitronectin and peptide (i.e., arginine-glycine-aspartic acid cysteine (RGDC)) enhanced bone cell adhesion and production of extracellular matrix [ 403 ].…”

Sintesis Komposit Hidroksiapatit-Lantanum Oksida (HA-La2O3) dengan Metode Hidrotermal secara In-Situ dan Ex-Situ

“…Khusus komposit Hidroksiapatit (HA) telah lama digunakan sebagai biomaterial penambal gigi karena memiliki sifat estetika dan keamanan yang lebih baik dibandingkan amalgam (Lavigueuer & Zhu, 2012), (Bartonickova et al, 2017). Untuk dapat diaplikasikan sebagai biokermaik penambal gigi, komposit HA harus memiliki ukuran butir yang sesuai, hal ini dapat diatur melalui proses sintesisnya (Bozkurt et al, 2015).…”