Porous polycaprolactone scaffold for cardiac tissue engineering fabricated by selective laser sintering

Yeong, Wai Yee; Sudarmadji, N.; Yu, Haiyue; Chua, Chee Kai; Leong, Kah Fai; Venkatraman, Subbu S.; Boey, Yin Chiang Freddy; Tan, Lay Poh

doi:10.1016/j.actbio.2009.12.033

Cited by 319 publications

(190 citation statements)

References 44 publications

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“…Biomaterials considered for tissue engineering scaffold fabricated via SLS include polycaprolactone (PCL) [12,[19][20][21][22][23][24], polyetheretherketone (PEEK) [17], polylactide acid (PLA) [25] and poly(lacticco-glycolide) (PLG) [26]. As these materials may not stimulate sufficient bone ingrowth on their own, bioactive components can be added to enhance bone ingrowth, cell attachment, etc.…”

Section: Introductionmentioning

confidence: 99%

Fabrication, mechanical and in vivo performance of polycaprolactone/tricalcium phosphate composite scaffolds

et al. 2012

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In this paper, the use of the Selective Laser Sintering (SLS) process for the generation of bone tissue engineering scaffolds from PCL and PCL/TCP is explored. Different scaffold designs are generated and are assessed from the point of view of manufacturability, porosity and mechanical performance. Large scaffold specimens are generated, with a preferred design, and are assessed through an in vivo study in a critical size bone defect in the sheep tibia with subsequent microscopic, histological and mechanical evaluation. Further explorations are performed to generate scaffolds with increasing TCP contents.Scaffold fabrication from PCL and PCL/TCP mixtures with up to 50 mass-% TCP is shown to be possible. With increasing macroporosity the stiffness of the scaffolds is seen to drop, however, the stiffness can be increased by minor geometrical changes, such as the addition of a cage around the scaffold. In the animal study the selected scaffold for implantation did not perform as well as the TCP control in terms of new bone formation and the resulting mechanical performance of the defect area. A possible cause for this is presented.

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Section: Introductionmentioning

confidence: 99%

Fabrication, mechanical and in vivo performance of polycaprolactone/tricalcium phosphate composite scaffolds

et al. 2012

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“…In contrast, the indirect printing method it is ideally suited for the controlled creation of the highly organized, aligned micro‐architecture and patient specific geometries that cardiac tissue engineering requires that cannot be generated with the traditional methods, such as solvent casting and particle leaching 12, 13, 14, 15…”

Section: Introductionmentioning

confidence: 99%

Indirect three‐dimensional printing: A method for fabricating polyurethane‐urea based cardiac scaffolds

Hernández‐Córdova

Mathew

Balint

et al. 2016

J. Biomed. Mater. Res.

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Biomaterial scaffolds are a key part of cardiac tissue engineering therapies. The group has recently synthesized a novel polycaprolactone based polyurethane‐urea copolymer that showed improved mechanical properties compared with its previously published counterparts. The aim of this study was to explore whether indirect three‐dimensional (3D) printing could provide a means to fabricate this novel, biodegradable polymer into a scaffold suitable for cardiac tissue engineering. Indirect 3D printing was carried out through printing water dissolvable poly(vinyl alcohol) porogens in three different sizes based on a wood‐stack model, into which a polyurethane‐urea solution was pressure injected. The porogens were removed, leading to soft polyurethane‐urea scaffolds with regular tubular pores. The scaffolds were characterized for their compressive and tensile mechanical behavior; and their degradation was monitored for 12 months under simulated physiological conditions. Their compatibility with cardiac myocytes and performance in novel cardiac engineering‐related techniques, such as aggregate seeding and bi‐directional perfusion, was also assessed. The scaffolds were found to have mechanical properties similar to cardiac tissue, and good biocompatibility with cardiac myocytes. Furthermore, the incorporated cells preserved their phenotype with no signs of de‐differentiation. The constructs worked well in perfusion experiments, showing enhanced seeding efficiency. © 2016 The Authors Journal of Biomedical Materials Research Part A Published by Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1912–1921, 2016.

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“…An alternative to produce uniform pore size is to use computer aided technology to design scaffolds with defined pore structure and size [49] . Additive manufacturing techniques, such as selective laser sintering (SLS) [20] fabricate porous scaffold struture layer-by-layer with stiffness of fabricated scaffold similar to native human myocardium (0.2 mPa). Inkjet printing technique was also employed for indirect fabrication of scaffolds for tissue engineering [50] .…”

Section: Conventional Techniques In Cardiac Tissue Engineeringmentioning

confidence: 99%

“…Specifically, cardiac tissue engineering aims to provide biological solutions to restore failing hearts and has one of the largest potential in regenerative medicine [17] . With advancement of three-dimensional (3D) printing, also known as additive manufacturing, various techniques have been applied in producing patientspecific biological substitutes, ranging from orthopedic implants [18][19] to scaffolds for tissue engineering [14,20] . Coupled with computer-aided technology, customized patch-like scaffold can be designed using additive manufacturing for cell delivery.…”

Section: Introductionmentioning

confidence: 99%

Bioprinting in cardiovascular tissue engineering: a review

Lee¹,

Sing²,

Tan³

et al. 2016

IJB

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Fabrication techniques for cardiac tissue engineering have been evolving around scaffold-based and scaffold-free approaches. Conventional fabrication approaches lack control over scalability and homogeneous cell distribution. Bioprinting provides a technological platform for controlled deposition of biomaterials, cells, and biological factors in an organized fashion. Bioprinting is capable of alternating heterogeneous cell printing, printing anatomical relevant structure and microchannels resembling vasculature network. These are essential features of an engineered cardiac tissue. Bioprinting can potentially build engineered cardiac construct that resembles native tissue across macro to nanoscale.

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Porous polycaprolactone scaffold for cardiac tissue engineering fabricated by selective laser sintering

Cited by 319 publications

References 44 publications

Fabrication, mechanical and in vivo performance of polycaprolactone/tricalcium phosphate composite scaffolds

Fabrication, mechanical and in vivo performance of polycaprolactone/tricalcium phosphate composite scaffolds

Indirect three‐dimensional printing: A method for fabricating polyurethane‐urea based cardiac scaffolds

Bioprinting in cardiovascular tissue engineering: a review

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