Porous titanium and Ti–35Nb alloy: effects on gene expression of osteoblastic cells derived from human alveolar bone

Prado, Renata Falchete do; Rabêlo, Sylvia Bicalho; Andrade, Dennia Perez de; Nascimento, Rodrigo Dias; Henriques, Vinícius André Rodrigues; Carvalho, Yasmin Rodarte; Cairo, Carlos Alberto Alves; Vasconcellos, Luana Marotta Reis de

doi:10.1007/s10856-015-5594-0

Cited by 21 publications

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“…Ti-6Al-4V alloy has other disadvantages regarding the toxicity of its basic elements [ 10 ]. The development of new titanium alloys, which exhibit only biocompatible elements, aim to improve the properties of the material, such as corrosion resistance, biocompatibility, elastic modulus and ductility [ 3 , 11 , 12 ]. Binary or tertiary alloys containing the basic elements of niobium (Nb) and zirconium (Zr) have been investigated for their biocompatibility [ 3 , 13 , 14 ] and also because they present high corrosion resistance and lower elastic modulus than Ti-6Al-4V and Ti-6Al-7Nb alloys [ 7 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

In vitro and in vivo biological performance of porous Ti alloys prepared by powder metallurgy

et al. 2018

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Titanium (Ti) and Ti-6 Aluminium-4 Vanadium alloys are the most common materials in implants composition but β type alloys are promising biomaterials because they present better mechanical properties. Besides the composition of biomaterial, many factors influence the performance of the biomaterial. For example, porous surface may modify the functional cellular response and accelerate osseointegration. This paper presents in vitro and in vivo evaluations of powder metallurgy-processed porous samples composed by different titanium alloys and pure Ti, aiming to show their potential for biomedical applications. The porous surfaces samples were produced with different designs to in vitro and in vivo tests. Samples were characterized with scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS) and elastic modulus analyses. Osteogenic cells from newborn rat calvaria were plated on discs of different materials: G1—commercially pure Ti group (CpTi); G2—Ti-6Al-4V alloy; G3—Ti-13 Niobium-13 Zirconium alloy; G4—Ti-35 Niobium alloy; G5—Ti-35 Niobium-7 Zirconium-5 Tantalum alloy. Cell adhesion and viability, total protein content, alkaline phosphatase activity, mineralization nodules and gene expression (alkaline phosphatase, Runx-2, osteocalcin and osteopontin) were assessed. After 2 and 4 weeks of implantation in rabbit tibia, bone ingrowth was analyzed using micro-computed tomography (μCT). EDS analysis confirmed the material production of each group. Metallographic and SEM analysis revealed interconnected pores, with mean pore size of 99,5μm and mean porosity of 42%, without significant difference among the groups (p>0.05). The elastic modulus values did not exhibit difference among the groups (p>0.05). Experimental alloys demonstrated better results than CpTi and Ti-6Al-4V, in gene expression and cytokines analysis, especially in early experimental periods. In conclusion, our data suggests that the experimental alloys can be used for biomedical application since they contributed to excellent cellular behavior and osseointegration besides presenting lower elastic modulus.

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Section: Introductionmentioning

confidence: 99%

In vitro and in vivo biological performance of porous Ti alloys prepared by powder metallurgy

et al. 2018

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“…Samples production was described in previous studies [7,8]. Briefly pure titanium grade II powder was obtained by the hydrogenation/ dehydrogenation technique (HDH).…”

Section: Titanium Samplesmentioning

confidence: 99%

“…Cell isolation from bone explants were performed as previously described [8]. Briefly, bone fragments were kept in a supplemented total medium (MTSα-MEM), supplemented with 10% of fetal bovine serum (Gibco-Invitrogen Corporation, New York, USA), 50μg/ mL of gentamicin, 0.3μg/mL of fungizone, 10-7M of dexamethasone (Sigma-Aldrich St Louis MO, USA), 5 μg/mL of ascorbic acid (Mallinckrodt Chemicals, Phillipsburg, UK) and 7mM of betaglycerophosphate (Sigma-Aldrich St Louis MO, USA), in a CO2chamber(Ultrasafe HF 212UV Instrulab, São Paulo, Brazil).…”

Section: Biological Assaymentioning

confidence: 99%

“…It was performed the sequential enzyme digestion of bone fragments with type II collagenase (Gibco-Invitrogen Corporation, New York, USA). Isolated cells and explants were maintained in a humid chamber at 5% of CO2 and 37oC, with medium change at every 72 hours for nearly 15 days, until cell confluence [8].…”

Section: Biological Assaymentioning

confidence: 99%

“…The cells were enzymatically detached using a solution of trypsin (Gibco-Invitrogen Corporation, New York, USA), EDTA1mM (Invitrogen, California, USA) and colagenase II, centrifuged, resuspended, and counted in a Neubauer chamber. Cells at passage 3-7 were plated on each group of titanium disks in 24-well polystyrene plates (Corning Incorporated, New York, USA), at a density of 20,000 cells/well [8]. In control group cells were plated on polystyrene bottom of wells,according Figure 2.…”

Section: Biological Assaymentioning

confidence: 99%

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Expression of BMP II by human osteoblasts cultivated on dense or porous titanium

Gagliardi

Vasconcellos

Cairo³

et al. 2018

BDS

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Objective: To quantified the expression of the morphogenetic bone protein II (BMP II) produced by human cells with osteoblast differentiation, after cultured over dense or porous samples of pure titanium grade II. Material and methods: The samples were produced by powder metallurgy technique. Mesenquimal steam cells isolated from alveolar bone of healthy donors were stimulated to differentiate, assuming an osteoblastic phenotype, by supplemented medium and plated over the samples. The experimental groups were: control, dense titanium, titanium of higher porosity and titanium of lower porosity. After 7 and 14 days, the RNA was extracted. The cDNA was prepared and the polymerase chain reaction (PCR) technique was used in real time, using the constitutive gene Beta-Actin to relative quantification. The pore morphology and interconnection were confirmed by Scanning Electron Microscopy (SEM). Results: Total porosity (obtained after apply dimensions and density formulas) and surface porosity (SEM) presented significant differences among the groups. For the group of total porosity of 33.79%, the superficial porosity was 32.5% (± 7.74%) and for the group of 41.79%, the superficial porosity was 37.4% (± 7.95%), significantly lower. The expression of BMP II was similar in all groups. Conclusion: It was concluded that the powder metallurgy has a reduced ability to standardize the porosity in the samples and that the porosity does not interfere in the cellular response of BMP II production, an important inducer of osteoblastic differentiation. KeywordsPorous titanium; BMP II; Cell culture; PCR.

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Electrospun polylactic acid scaffolds with strontium‐ and cobalt‐doped bioglass for potential use in bone tissue engineering applications

et al. 2022

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The development of nanoscale biomaterials associated with polymers has been growing over the years, due to their important structural characteristics for applications in biological systems. The present study aimed to produce and test polymeric scaffolds composed of polylactic acid (PLA) fibers associated with a 58S bioglass doped with therapeutic ions for use in tissue engineering. Three 58S Bioglass was obtained by the sol-gel route, pure and doped with 5% strontium and cobalt ions. Solutions of 7% PLA was used as control and added the three different bioglass, 4% of 58S bioglass (PLA-BG), 4% bioglass-doped strontium (PLA-BGSr) and 4% bioglass-doped cobalt (PLA-BGCo). Scaffolds were produced through electrospinning process, and was characterized chemical and morphologically. The in vitro tests were performed using mesenchymal cells cultures from femurs of nine rats, grown in osteogenic supplemented total culture medium. After osteoblastic differentiation induction cell viability, alkaline phosphatase activity, total protein content quantification, and visualization of mineralization nodule tests were performed. Analysis of normal distribution used the Shapiro-Wilk test (nanofibers diameter and biological assay). Data were compared using the Kruskal-Wallis nonparametric test (p = 0.05). The bioglasses produced proved to be free of nitrate, chlorinated and nano-sized, with effective incorporation of therapeutic ions in their structure. All materials showed cell viability (>70%), total protein production, and alkaline phosphatase activity. It was possible to develop polylactic acid scaffolds associated with 58S bioglass doped with therapeutic ions without cytotoxicity. Scaffolds characteristics appear to sustain its application in bone tissue engineering.

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Porous titanium and Ti–35Nb alloy: effects on gene expression of osteoblastic cells derived from human alveolar bone

Cited by 21 publications

References 46 publications

In vitro and in vivo biological performance of porous Ti alloys prepared by powder metallurgy

In vitro and in vivo biological performance of porous Ti alloys prepared by powder metallurgy

Expression of BMP II by human osteoblasts cultivated on dense or porous titanium

Electrospun polylactic acid scaffolds with strontium‐ and cobalt‐doped bioglass for potential use in bone tissue engineering applications

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