Porphyrin-xylan-coated silica nanoparticles for anticancer photodynamic therapy

Bouramtane, Soukaina; Bretin, Ludovic; Pinon, Aline; Léger, David Y.; Liagre, Bertrand; Richard, Laurence; Brégier, Frédérique; Sol, Vincent; Chaleix, Vincent

doi:10.1016/j.carbpol.2019.02.070

Cited by 44 publications

(39 citation statements)

References 38 publications

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“…IC 50 values were calculated in order to compare free TPPOH-PDT vs. TPPOH-X SNPs-PDT. We observed that TPPOH-X SNPs-PDT was much more effective than free TPPOH-PDT in HT-29 cells with 10.8-fold more cytotoxicity with IC 50 values of 550.2 nM for TPPOH-X SNPs-PDT and around 6 μM for free TPPOH-PDT [29]. Similar results were observed in HCT116 (40.5-fold) and SW620 (39.5-fold) cell lines with respective IC 50 values of 72.6 and 75.4 nM for TPPOH-X SNPs-PDT and around 3 μM for free TPPOH-PDT.…”

Section: Resultsmentioning

confidence: 99%

“…The synthesis and characterization of free TPPOH and TPPOH-X SNPs were recently published by our research team [29]. SNPs were synthesized with an 80 nm average diameter following the modified Stöber method.…”

Section: Methodsmentioning

confidence: 99%

“…The presence of glucuronic acid groups on xylan results in the formation of ionic bonds on the surface of SNPs which is made cationic by (3-aminopropyl) triethoxysilane (APTES). SNPs vectorization did not induced changes in the TPPOH spectrum (supplementary data [29]). Stock solutions of free TPPOH (5 mg/mL) and TPPOH-X SNPs (20 mg/mL) were prepared in ethanol.…”

Section: Methodsmentioning

confidence: 99%

“…In the current paper, we investigated the anticancer efficacy of the PS 5-(4-hydroxyphenyl)-10,15,20-triphenylporphyrin (TPPOH). According to our research program on polysaccharide modifications with PS for PDT applications, we explored the newly synthesized core-shell hybrid SNPs based on xylan for the targeted delivery of TPPOH [29]. First, we showed a strong anticancer efficacy of TPPOH in human CRC cell lines, which has not been described before.…”

Section: Introductionmentioning

confidence: 99%

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Photodynamic Therapy Activity of New Porphyrin-Xylan-Coated Silica Nanoparticles in Human Colorectal Cancer

Bretin

Pinon

Bouramtane

et al. 2019

Cancers

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Photodynamic therapy (PDT) using porphyrins has been approved for treatment of several solid tumors due to the generation of cytotoxic reactive oxygen species (ROS). However, low physiological solubility and lack of selectivity towards tumor sites are the main limitations of their clinical use. Nanoparticles are able to spontaneously accumulate in solid tumors through an enhanced permeability and retention (EPR) effect due to leaky vasculature, poor lymphatic drainage, and increased vessel permeability. Herein, we proved the added value of nanoparticle vectorization on anticancer efficacy and tumor-targeting by 5-(4-hydroxyphenyl)-10,15,20-triphenylporphyrin (TPPOH). Using 80 nm silica nanoparticles (SNPs) coated with xylan-TPPOH conjugate (TPPOH-X), we first showed very significant phototoxic effects of TPPOH-X SNPs mediated by post-PDT ROS generation and stronger cell uptake in human colorectal cancer cell lines compared to free TPPOH. Additionally, we demonstrated apoptotic cell death induced by TPPOH-X SNPs-PDT and the interest of autophagy inhibition to increase anticancer efficacy. Finally, we highlighted in vivo, without toxicity, elevated anticancer efficacy of TPPOH-X SNPs through improvement of tumor-targeting compared to a free TPPOH protocol. Our work demonstrated for the first time the strong anticancer efficacy of TPPOH in vitro and in vivo and the merit of SNPs vectorization.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Photodynamic Therapy Activity of New Porphyrin-Xylan-Coated Silica Nanoparticles in Human Colorectal Cancer

Bretin

Pinon

Bouramtane

et al. 2019

Cancers

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“…12 Xylans from different sources have been used in the biosynthesis of grafts, bandages and sutures, synthesis of micro and nanoparticles 13,14 as well as for the controlled release of drugs. [15][16][17] It has been reported in the literature that corn cobs (Gramineae), underutilized in industry and often discarded, are the most promising material for the extraction of these biopolymers. 18 It has been documented that xylans obtained from corn cobs had a chemical composition consisting of 4-Omethyl-D-glucuronic acid, L-arabinose and D-xylose in a ratio of 2:7:19.…”

Section: Introductionmentioning

confidence: 99%

<p>Synthesis of Silver Nanoparticle Employing Corn Cob Xylan as a Reducing Agent with Anti-<em>Trypanosoma cruzi</em> Activity</p>

Pinto¹,

Viana²,

Moreno³

et al. 2020

IJN

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Background: Chagas disease, also known as American Trypanosomiasis, is caused by the protozoan Trypanosoma cruzi. It is occurring in Americas, including USA and Canada, and Europe and its current treatment involves the use of two drugs as follows: benznidazole (BNZ) and nifurtimox, which present high toxicity and low efficacy during the chronic phase of the disease, thus promoting the search for more effective therapeutic alternatives. Amongst them xylan, a bioactive polysaccharide, extracted from corn cob. Methods: Ultraviolet-visible spectroscopy, Fourier transform infrared spectroscopy (FITR), Raman spectroscopy, energy-dispersive X-ray spectroscopy (EDS), scanning electron microscopy, atomic force microscopy, plasma optical emission spectroscopy (ICP-OES), dynamic light scattering (DLS) have been used to characterize the silver-xylan nanoparticles (NX). Their cytotoxicity was evaluated with 3-bromo(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) test. MTT and flow cytometry were used to ascertain the anti-Trypanosoma cruzi activity. Results: UV-Vis spectroscopy gave plasmon resonance ranging between 400 and 450 nm while FITC and Raman spectroscopy proved nano interface functionalized with xylan. ICP-OES data showed NX with xylan (81%) and silver (19%). EDS showed NX consisting of carbon (59.4%), oxygen (26.2%) and silver (4.8%) main elements. Spherical NX of 55 nm average size has been depicted with SEM and AFM, while DLS showed 102 ± 1.7 nm NX. The NX displayed negligible cytotoxicity (2000 µg/mL). NX (100 µg/mL) was more effective, regardless of experiment time, in affecting the ability of parasites to reduce MTT than BZN (100 µg/mL). In addition, NX (100 µg/mL) induced death of 95% of parasites by necrosis. Conclusion: This is the first time silver nanoparticles are presented as an anti-Trypanosoma cruzi agent and the data point to the potential application of NX to new preclinical studies in vitro and in vivo.

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Acetylxylan‐pheophorbide‐a nanoparticles designed for tumor‐targeted photodynamic therapy

Bouramtane

Bretin

Pinon

et al. 2021

J of Applied Polymer Sci

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The poor solubility of most photosensitizers (PS) hampers their practical use as clinical agents. Conjugation of PS with water‐soluble nanoparticles has been often used to bypass this hurdle. We describe in this study the extraction of glucuronoacetylxylan from chestnut sawdust, the hemisynthesis of pheophorbide‐a (Pha) and its covalent attachment to xylan. The resulting conjugates (xyl‐Pha) spontaneously form water‐soluble spherical nanoparticles (xyl‐Pha NPs) ranging between 100 and 250 nm in diameter. Assayed against the HT‐29 colorectal cancer cell line, xyl‐Pha NPs exhibited a dose‐dependent phototoxicity only when irradiated with red light.

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Porphyrin-xylan-coated silica nanoparticles for anticancer photodynamic therapy

Cited by 44 publications

References 38 publications

Photodynamic Therapy Activity of New Porphyrin-Xylan-Coated Silica Nanoparticles in Human Colorectal Cancer

Photodynamic Therapy Activity of New Porphyrin-Xylan-Coated Silica Nanoparticles in Human Colorectal Cancer

<p>Synthesis of Silver Nanoparticle Employing Corn Cob Xylan as a Reducing Agent with Anti-<em>Trypanosoma cruzi</em> Activity</p>

Acetylxylan‐pheophorbide‐a nanoparticles designed for tumor‐targeted photodynamic therapy

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