Porphyromonas gingivalis resistance and virulence: An integrated functional network analysis

Sao, Prachi; Vats, Siddharth; Singh, Sachidanand

doi:10.1016/j.gene.2022.146734

Cited by 8 publications

(5 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…P. gingivalis is one of the major pathogens responsible for severe and chronic manifestations of periodontal disease, producing a number of virulence factors that cause direct and indirect destruction to periodontal tissues through modulation of the host inflammatory response [29]. Although human gingival epithelium prevents intrusion by periodontal bacteria, P. gingivalis is able to invade gingival epithelial cells.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Antimicrobial and Antibiofilm Properties and Bioaccessibility after Oral Digestion of Chemically Characterized Extracts Obtained from Cistus × incanus L., Scutellaria lateriflora L., and Their Combination

Ullah

Minno

Filippis

et al. 2023

Foods

View full text Add to dashboard Cite

Periodontal diseases are oral inflammatory diseases ranging from gingivitis to chronic periodontitis. Porphyromonas gingivalis is one of the major pathogens responsible for severe and chronic periodontitis. Plant extracts with antimicrobial activity could be considered possible alternatives to chlorhexidine, an antiseptic substance used in oral hygiene thatcan cause bacteria resistance. Here, two commercial extracts obtained from Cistus × incanus L. and Scutellaria lateriflora L. were chemically characterized usingUltra-High-Performance Liquid Chromatography (UHPLC) coupled with a Q-Exactive Hybrid Quadrupole Orbitrap Mass Spectrometer. The extracts were studied for their bioaccessibility after simulated in vitro oral digestion, their antimicrobial activity against P. gingivalis, their protective effects against cellular invasion by P. gingivalis, and their antibiofilm activity. The extracts were found to contain very complex mixtures of polyphenols, which were quite stable after in vitro simulated oral digestion and demonstrated mild, dose-dependent inhibitory activity against P. gingivalis growth. This activity increased with the combination of the two extracts. Moreover, the combination of the extracts induced a reduction in P. gingivalis HaCaT invasiveness, and the reduction in biofilm came to around 80%. In conclusion, a combination of C. incanus and S. lateriflora showed promising effects useful in the treatment of gingivitis.

show abstract

Section: Discussionmentioning

confidence: 99%

In Vitro Antimicrobial and Antibiofilm Properties and Bioaccessibility after Oral Digestion of Chemically Characterized Extracts Obtained from Cistus × incanus L., Scutellaria lateriflora L., and Their Combination

Ullah

Minno

Filippis

et al. 2023

Foods

View full text Add to dashboard Cite

show abstract

“…Networks can be used to display a wide range of biological data, including protein–protein interactions, gene regulation, cellular pathways, and signal transductions [ 10 , 46 ]. An interaction network is represented as a graph

, where

and

are the sets of vertices (nodes/genes/proteins) and edges (links/functional associations/interactions), respectively [ 47 ].…”

Section: Methodsmentioning

confidence: 99%

“…Furthermore, research shows that RA and OA significantly alter the oral microbiome [ 3 ]. Periodontal disease (PD) is a chronic inflammatory disease of the tooth and supporting tissues that has been linked to a particular group of bacteria, one of which is Porphyromonas gingivalis [ 9 , 10 , 11 ]. Chronic periodontitis is usually characterized by a heavy infiltrate of inflammatory cells, including macrophages, in the gingival tissue; it results in the resorption of alveolar bone and other tooth-supporting tissues.…”

Section: Introductionmentioning

confidence: 99%

Classifying Integrated Signature Molecules in Macrophages of Rheumatoid Arthritis, Osteoarthritis, and Periodontal Disease: An Omics-Based Study

Sao

Chand

Al-Keridis

et al. 2022

CIMB

Self Cite

View full text Add to dashboard Cite

Rheumatoid arthritis (RA), osteoarthritis (OA), and periodontal disease (PD) are chronic inflammatory diseases that are globally prevalent, and pose a public health concern. The search for a potential mechanism linking PD to RA and OA continues, as it could play a significant role in disease prevention and treatment. Recent studies have linked RA, OA, and PD to Porphyromonas gingivalis (PG), a periodontal bacterium, through a similar dysregulation in an inflammatory mechanism. This study aimed to identify potential gene signatures that could assist in early diagnosis as well as gain insight into the molecular mechanisms of these diseases. The expression data sets with the series IDs GSE97779, GSE123492, and GSE24897 for macrophages of RA, OA synovium, and PG stimulated macrophages (PG-SM), respectively, were retrieved and screened for differentially expressed genes (DEGs). The 72 common DEGs among RA, OA, and PG-SM were further subjected to gene–gene correlation analysis. A GeneMANIA interaction network of the 47 highly correlated DEGs comprises 53 nodes and 271 edges. Network centrality analysis identified 15 hub genes, 6 of which are DEGs (API5, ATE1, CCNG1, EHD1, RIN2, and STK39). Additionally, two significantly up-regulated non-hub genes (IER3 and RGS16) showed interactions with hub genes. Functional enrichment analysis of the genes showed that “apoptotic regulation” and “inflammasomes” were among the major pathways. These eight genes can serve as important signatures/targets, and provide new insights into the molecular mechanism of PG-induced RA, OA, and PD.

show abstract

“…Esta sobreexpresión también afecta otras funciones de la bomba, como la capacidad de liberar factores virulentos. A pesar de que los factores sigma ECF son los sistemas reguladores más comunes, permiten que las bacterias se adapten a diferentes entornos y situaciones difíciles, uniéndose a una región promotora específica que activa la ARN polimerasa y aumenta la expresión de factores de virulencia (23).…”

Section: Artículo Originalunclassified

Impacto de la resistencia bacteriana en la elección de antibióticos en odontología: una revisión de las tendencias actuales

Santaella Palma,

Bazurto Quinteros,

Ramírez Sánchez

2024

Más Vita. Rev. Cienc. Salud

View full text Add to dashboard Cite

Introducción: La reiterada exposición de las distintas bacterias a diversos grupos de antibióticos ha provocado un aumento representativo de la resistencia antimicrobiana, constituyendo un problema de salud pública global. Objetivo: Describir el impacto de la resistencia bacteriana en la elección de antibióticos en odontología: una revisión de las tendencias actuales. Materiales y métodos: Se aplicó una revisión narrativa, no sistemática, referente al impacto de la resistencia bacteriana en la elección de antibióticos en odontología, que incluyó 34 trabajos científicos, conformados por estudios observacionales, informes y artículos de revisión. La búsqueda y recolección de la información se efectuó en las bases de datos: PubMed, Medline, Google académico, Science Direct y Scopus-Elsevier, durante noviembre y diciembre de 2023. Resultados: La resistencia antimicrobiana se debe a algunos mecanismos propios de las células bacterianas como: eliminación de antibióticos por bombas de extrusión de la membrana celular, reducción de la absorción de antimicrobianos, enzimas que generan la inactivación o degradación de antibióticos, así como las mutaciones o alteraciones en el sitio diana de los antibióticos, por lo que es necesaria la aplicación de estrategias innovadoras para contrarrestar la creciente resistencia bacteriana. Conclusión: La capacidad de las bacterias de la cavidad bucal para formar biopelículas dentales, genera un hábitat propicio para el intercambio genético, que favorece la transmisión de genes de resistencia. Por lo cual, actualmente se desarrollan estrategias alternativas, que incluyen agentes antimicrobianos y antibiofilm. La investigación científica se convierte en una necesidad imperativa para abordar este desafío terapéutico en el ámbito odontológico.

show abstract

Porphyromonas gingivalis resistance and virulence: An integrated functional network analysis

Cited by 8 publications

References 72 publications

In Vitro Antimicrobial and Antibiofilm Properties and Bioaccessibility after Oral Digestion of Chemically Characterized Extracts Obtained from Cistus × incanus L., Scutellaria lateriflora L., and Their Combination

In Vitro Antimicrobial and Antibiofilm Properties and Bioaccessibility after Oral Digestion of Chemically Characterized Extracts Obtained from Cistus × incanus L., Scutellaria lateriflora L., and Their Combination

Classifying Integrated Signature Molecules in Macrophages of Rheumatoid Arthritis, Osteoarthritis, and Periodontal Disease: An Omics-Based Study

Impacto de la resistencia bacteriana en la elección de antibióticos en odontología: una revisión de las tendencias actuales

Contact Info

Product

Resources

About