2020
DOI: 10.1039/d0ra05293k
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Portable environment-signal detection biosensors with cell-free synthetic biosystems

Abstract: By embedding the regulated genetic circuits and cell-free systems onto a paper, a portable in vitro biosensing platform has been established.

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Cited by 12 publications
(7 citation statements)
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“…Therefore, plasmids can be extracted from V. natriegens , C. glutamate and B. subtilis , respectively, and various expression vectors can be designed to further increase the expression of CFPS systems. In addition, studies on the metabolic pathways, gene circuits and diagnostic tools (Cho and Lu 2020 ; Lin et al 2021 ; Lin et al 2020a , b ; Yang and Lu 2020 ) in the E. coli CFPS system can be tried in the other three systems, and a diversified CFPS platform can be developed further to expand the application scope of the CFPS systems.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…Therefore, plasmids can be extracted from V. natriegens , C. glutamate and B. subtilis , respectively, and various expression vectors can be designed to further increase the expression of CFPS systems. In addition, studies on the metabolic pathways, gene circuits and diagnostic tools (Cho and Lu 2020 ; Lin et al 2021 ; Lin et al 2020a , b ; Yang and Lu 2020 ) in the E. coli CFPS system can be tried in the other three systems, and a diversified CFPS platform can be developed further to expand the application scope of the CFPS systems.…”
Section: Discussionmentioning
confidence: 99%
“…Therefore, they are increasingly used in the production of complex protein products with low expression rates, aggregation, toxicity, and poor solubility in vivo (Schoborg et al 2018 ). CFPS systems also have broad application prospects and have been used in the design of rapid prototyping of DNA regulatory elements, logic systems (Chappell et al 2013 ; Karim and Jewett 2016 ; Stech and Kubick 2015 ), and biosensor devices (Lin et al 2020a , b ; Pardee et al 2016 ; Zhang et al 2020 , 2019 ).…”
Section: Introductionmentioning
confidence: 99%
“…Upon rehydration of biocomponents, reporter expression can be initiated allowing target detection either with a colorimetric output visible by the naked eye, or with a fluorescent output detectable with a portable light source. Adapted with permission from [47].…”
Section: Synthetic Cell-based Biosensors For Environmental Toxins And...mentioning
confidence: 99%
“…A typical approach is achieved by using sensing proteins (e.g., ligand responsive TFs) or nucleic acids (e.g., riboswitches, aptamers) in cell-free protein synthesis (CFPS) systems [42]. To date many studies have utilized TF-based gene circuits to demonstrate proof-of-concept applications of cell-free expression systems to detect contaminants such as heavy metals, pathogens, or antibiotics [47][48][49] using either crude cell extracts or purified protein components (PURE). Similarly, aptamer-based cell-free systems have also been shown to act as efficient biosensors.…”
Section: Cell-free Expression System-based Biosensorsmentioning
confidence: 99%
“…[15] Paper-based cell-free biosensors also enable the monitoring of small molecules. In recent studies, cell-free sensing modules employing aTFs have been applied in the detection of heavy metals, such as mercury, [258,259] lead, [259] and arsenic, [260] as well as fluoride [261] ions in water. Briefly, the presence of target ions in a sample causes conformational changes in aTFs, which results in their release from DNA, and induces the transcription and translation of fluorescent genes (Figure 7).…”
Section: Paper-based Cell-free Biosensorsmentioning
confidence: 99%