Portable in situ temperature‐dependent spectroscopy on a low‐cost microfluidic platform integrated with a battery‐powered thermofoil heater

Krishna, Katla Sai; Zhou, Wan; Tavakoli, Hamed; Méndez, Elvia Lilia Padilla; Li, Xiujun

doi:10.1002/viw.20220053

Cited by 10 publications

(4 citation statements)

References 36 publications

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“…However, this process is unfriendly to operation, and may cause droplet loss and contamination. Hence, in situ heating of the chip is expected, 19 which would reduce sample loss and improve reproducibility. Therefore, we designed a thermal cycling module in our control instrument.…”

Section: Resultsmentioning

confidence: 99%

A three-in-one microfluidic droplet digital PCR platform for absolute quantitative analysis of DNA

Ren

Zhang

et al. 2023

Lab Chip

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Section: Resultsmentioning

confidence: 99%

A three-in-one microfluidic droplet digital PCR platform for absolute quantitative analysis of DNA

Ren

Zhang

et al. 2023

Lab Chip

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“…The depth of engraving is determined by the laser’s speed and power, both controlled by software ( Hubeatir et al, 2018 ; Mansour et al, 2022 ). The laser moves back and forth over the substrate to create the desired microfluidic channels by removing material in a controlled manner ( Yao & Fan, 2021 ; Katla et al, 2023 ).…”

Section: Manufacturing Techniquesmentioning

confidence: 99%

Breaking the clean room barrier: exploring low-cost alternatives for microfluidic devices

Rodríguez

Andrade-Pérez

Vargas

et al. 2023

Front. Bioeng. Biotechnol.

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Microfluidics is an interdisciplinary field that encompasses both science and engineering, which aims to design and fabricate devices capable of manipulating extremely low volumes of fluids on a microscale level. The central objective of microfluidics is to provide high precision and accuracy while using minimal reagents and equipment. The benefits of this approach include greater control over experimental conditions, faster analysis, and improved experimental reproducibility. Microfluidic devices, also known as labs-on-a-chip (LOCs), have emerged as potential instruments for optimizing operations and decreasing costs in various of industries, including pharmaceutical, medical, food, and cosmetics. However, the high price of conventional prototypes for LOCs devices, generated in clean room facilities, has increased the demand for inexpensive alternatives. Polymers, paper, and hydrogels are some of the materials that can be utilized to create the inexpensive microfluidic devices covered in this article. In addition, we highlighted different manufacturing techniques, such as soft lithography, laser plotting, and 3D printing, that are suitable for creating LOCs. The selection of materials and fabrication techniques will depend on the specific requirements and applications of each individual LOC. This article aims to provide a comprehensive overview of the numerous alternatives for the development of low-cost LOCs to service industries such as pharmaceuticals, chemicals, food, and biomedicine.

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“…The laser spacing interval can be adjusted further to improve the surface finish of the material. The heat generated by the laser interaction with the acrylic removes the material by melting leaving behind the desired structure projected upwards from the ablated material surface [29,48,49]. The multi-axis movement of the laser (X swing and Y swing) resulted in the formation of engraved structures on the acrylic surface which was later used as a master mold.…”

Section: Fabrication Of Master Mold Using Laser Engravingmentioning

confidence: 99%

Design and fabrication of microfluidic devices: a cost-effective approach for high throughput production

Thomas,

Agrawal

2023

J. Micromech. Microeng.

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Microdevices have been recognized as a potential platform for performing numerous biomedical analysis and diagnostic applications. However, promising and viable techniques for a cost-effective and high throughput production of microfluidic devices still remain as a challenge. This paper addresses this problem with an alternative solution for the fabrication of microfluidic devices in a simple and efficient manner. We utilized laser-assisted engraving technique to fabricate a master mold on an acrylic sheet of different thicknesses from 4 to 20mm. Low cost indigenously developed CO2 (10.6μm wavelength) laser engraving device was used for the experiments. The effect of various laser parameters such as power and speed of operation on the height of engraved structures was studied in detail. Optimal engraving results were obtained with a laser speed of 200–250mm s−1 with a spacing interval of 0.002mm at a laser power of 10–12W. Master mold of microdevice with a channel width of 100μm were produced using this technique. The replica transfer was performed by a simple imprinting method using a benchtop universal testing machine that can provide a maximum compressive load upto 1kN. The replicas were successfully generated on various thin film substrates including polymers, plastics, Whatman filter paper, teflon, vinyl sheets, copper, and aluminum sheets. The effect of load applied on the depth of the microfluidic channel was studied for the substrates such as teflon and Whatman filter paper. A load of 1kN can generate a depth of a few hundred micrometers on various substrates mentioned above. The replicas were also transferred to thermoformable PETG (polyethylene terephthalate glycol) sheets under load with an elevated temperature. The channel-imprinted PETG substrates were later sandwiched between two acrylic sheets with adhesive-coated polymer sheets and screws at the corners. Soft lithographic techniques were also performed to replicate the channel on a poly dimethyl siloxane substrate which was later bonded to a glass plate using an oxygen plasma cleaner device. Fluidic flow testing was conducted by pumping dye-mixed deionized (DI) water through the channels using a syringe pump and connecting tubes at a constant flow rate of 5ml min−1. The outcomes of this study provide an alternative solution for a simple and low-cost method for microdevice fabrication at a large scale.

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Portable in situ temperature‐dependent spectroscopy on a low‐cost microfluidic platform integrated with a battery‐powered thermofoil heater

Cited by 10 publications

References 36 publications

A three-in-one microfluidic droplet digital PCR platform for absolute quantitative analysis of DNA

A three-in-one microfluidic droplet digital PCR platform for absolute quantitative analysis of DNA

Breaking the clean room barrier: exploring low-cost alternatives for microfluidic devices

Design and fabrication of microfluidic devices: a cost-effective approach for high throughput production

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