Portable point-of-care diagnostic devices

Zhang, Wei; Guo, Siyuan; Carvalho, Wildemar S.P.; Jiang, Yingying; Serpe, Michael J.

doi:10.1039/c6ay02158a

Cited by 61 publications

(29 citation statements)

References 136 publications

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“…12–14 Among its potential uses for different disease diagnosis, prototypes to elucidate cancer cell and tumor function have gained tremendous importance. 15, 16 Microfluidic-based analyzers for cell biomechanical screening and mechanophenotyping have promised a higher throughput compared to conventional tools and emerged as a clinically more relevant approach to single-cell analysis.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic iterative mechanical characteristics (iMECH) analyzer for single-cell metastatic identification

2017

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This study describes the development of a microfluidic biosensor called the iterative mechanical characteristics (iMECH) analyzer which enables label-free biomechanical profiling of individual cells for distinction between metastatic and non-metastatic human mammary cell lines. Previous results have demonstrated that pulsed mechanical nanoindentation can modulate the biomechanics of cells resulting in distinctly different biomechanical responses in metastatic and non-metastatic cell lines. The iMECH analyzer aims to move this concept into a microfluidic, clinically more relevant platform. The iMECH analyzer directs a cyclic deformation regimen by pulling cells through a test channel comprised of narrow deformation channels and interspersed with wider relaxation regions which together simulate a dynamic microenvironment. The results of the iMECH analysis of human breast cell lines revealed that cyclic deformations produce a resistance in non-metastatic 184A1 and MCF10A cells as determined by a drop in their average velocity in the iterative deformation channels after each relaxation. In contrast, metastatic MDA-MB-231 and MDA-MB-468 cells exhibit a loss of resistance as measured by a velocity raise after each relaxation. These distinctive modulatory mechanical responses of normal-like non-metastatic and metastatic cancer breast cells to the pulsed indentations paradigm provide a unique bio-signature. The iMECH analyzer represents a diagnostic microchip advance for discriminating metastatic cancer at the single-cell level.

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Section: Introductionmentioning

confidence: 99%

Microfluidic iterative mechanical characteristics (iMECH) analyzer for single-cell metastatic identification

2017

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“…The real-time virus detection has a high interest in various fields, such as biosecurity, biomedicine, and environmental science [104]. Nanosensor does not need trained personnel and expensive equipment, especially if obtained results can be read by naked eyes [105,106].…”

Section: Other Virusesmentioning

confidence: 99%

Virus detection using nanosensors

Saylan

Deni̇zli̇

2020

Nanosensors for Smart Cities

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“…To realize the full potential of POCT systems, both the speed and convenience of biosensing systems or device must be optimized [1][2][3]. Clinical diagnostics, for example, typically require long assay times and would benefit from immediately testing and diagnostics at the patient's site [1,[4][5][6]. Furthermore, in the case of cancer metastasis, it has been reported that circulating tumor cells (CTC) and excreted vesicles (50-150 nm in size) known as exosomes which have infiltrated the blood vessels can be measured and correlated to the level of metastasis [7][8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Electrospun Polymeric Microfiber Substrates for Rapid Protein and Cell-based Assays

Takai

Hoy

Yoshihara

2018

J. Photopol. Sci. Technol.

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Biosensing devices such as assays require both speed and sensitivity as two integral components for its effectiveness. Within this review, developed three-dimensional (3D) microfiber polystyrene (PS) platforms are leveraged to introduce a means for effectively rapid and sensitive assays. Considered broadly, the 3D structure contributes to an increased surface area for antibody immobilization while the natural hydrophobic nature of PS promotes this immobilization for immunoassay diagnostics. More specifically, rapid antigen capture can be realized by combining this platform with vacuum pressure to mitigate diffusion limitations for antibody-antigen interactions. Alternatively, the increased antibody immobilization density can realize the selective capture and release of circulating tumor cells (CTC). Through both low concentration CTC capture and effective release of said cells, patient-specific cancer care can be achieved.

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Portable point-of-care diagnostic devices

Cited by 61 publications

References 136 publications

Microfluidic iterative mechanical characteristics (iMECH) analyzer for single-cell metastatic identification

Microfluidic iterative mechanical characteristics (iMECH) analyzer for single-cell metastatic identification

Virus detection using nanosensors

Electrospun Polymeric Microfiber Substrates for Rapid Protein and Cell-based Assays

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