Portable Simultaneous Multiple Analyte Whole-Blood Analyzer for Point-of-Care Testing

Schembri, Carol T.; Ostoich, Vladimir; Lingane, Paul J.; Burd, T. L.; Buhl, Steven N.

doi:10.1093/clinchem/38.9.1665

Cited by 50 publications

(37 citation statements)

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“…Prominent examples of integrated microfluidic platforms where flow control elements coordinate the spatio-temporal arrangement of a range of LUOs towards sample-to-answer automation are based on rotationally induced centrifugal fields [17][18][19][20][21][22][23][24][25][26], electrokinetics [27], acoustophoresis [28][29][30][31], and digital (droplet) microfluidics on electrowetting-on-dielectric (EWOD) [32][33][34][35][36], surface acoustic waves (SAWs) [37][38][39] and multiphase flows [40][41][42], possibly supported by externally actuated precision pumps and mechanical valves. In this work, we focus on microfluidic technologies for automating typical in vitro procedures in life-science laboratories.…”

Section: Integrated Microfluidic Platformsmentioning

confidence: 99%

Efficient Development of Integrated Lab-On-A-Chip Systems Featuring Operational Robustness and Manufacturability

Ducrée

2019

Micromachines

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The majority of commercially oriented microfluidic technologies provide novel point-of-use solutions for laboratory automation with important areas in the context of the life sciences such as health care, biopharma, veterinary medicine and agrifood as well as for monitoring of the environment, infrastructures and industrial processes. Such systems are often composed of a modular setup exhibiting an instrument accommodating rather conventional actuation, detection and control units which interfaces with a fluidically integrated “Lab-on-a-Chip” device handling (bio-)sample(s) and reagents. As the complex network of tiny channels, chambers and surface-functionalised zones can typically not be properly cleaned and regenerated, these microfluidic chips are mostly devised as single-use disposables. The availability of cost-efficient materials and associated structuring, functionalisation and assembly schemes thus represents a key ingredient along the commercialisation pipeline and will be a first focus of this work. Furthermore, and owing to their innate variability, investigations on biosamples mostly require the acquisition of statistically relevant datasets. Consequently, intermediate numbers of consistently performing chips are already needed during application development; to mitigate the potential pitfalls of technology migration and to facilitate regulatory compliance of the end products, manufacture of such pilot series should widely follow larger-scale production schemes. To expedite and de-risk the development of commercially relevant microfluidic systems towards high Technology Readiness Levels (TRLs), we illustrate a streamlined, manufacturing-centric platform approach employing the paradigms of tolerance-forgiving Design-for-Manufacture (DfM) and Readiness for Scale-up (RfS) from prototyping to intermediate pilot series and eventual mass fabrication. Learning from mature industries, we further propose pursuing a platform approach incorporating aspects of standardisation in terms of specification, design rules and testing methods for materials, components, interfaces, and operational procedures; this coherent strategy will foster the emergence of dedicated commercial supply chains and also improve the economic viability of Lab-on-a-Chip systems often targeting smaller niche markets by synergistically bundling technology development.

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Section: Integrated Microfluidic Platformsmentioning

confidence: 99%

Efficient Development of Integrated Lab-On-A-Chip Systems Featuring Operational Robustness and Manufacturability

Ducrée

2019

Micromachines

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“…Plasma extraction in a CD-based format relies on the centrifugal force forcing the heavy cellular content towards the external edge of the CD, while the plasma is siphoned out by various valving systems. The success of the plasma separation in CD format relies on these valving systems, which have included passive siphoning, 200 hydrophobic, capillary, 201,202 or ferrowax microvalves.…”

Section: Formatmentioning

confidence: 99%

Micro-scale blood plasma separation: from acoustophoresis to egg-beaters

2013

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Plasma is a rich mine of various biomarkers including proteins, metabolites and circulating nucleic acids. The diagnostic and therapeutic potential of these analytes has been quite recently uncovered, and the number of plasma biomarkers will still be growing in the coming years. A significant part of the blood plasma preparation is still handled manually, off-chip, via centrifugation or filtration. These batch methods have variable waiting times, and are often performed under non-reproducible conditions that may impair the collection of analytes of interest, with variable degradation. The development of miniaturised modules capable of automated and reproducible blood plasma separation would aid in the translation of lab-on-a-chip devices to the clinical market. Here we propose a systematic review of major plasma analytes and target applications, alongside existing solutions for micro-scale blood plasma extraction, focusing on the approaches that have been biologically validated for specific applications.

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“…Our SSCE scheme involved a sample plug formation with single‐step operation that combined capillary action, capillary stop valves, and air vents. Capillary stop valves are widely used for liquid handling in compact‐disk‐format liquid handling devices . For these capillary stop valve applications, centrifugal force is used to handle the reagents.…”

Section: Introductionmentioning

confidence: 99%

Single‐step CE for miniaturized and easy‐to‐use system

2013

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We developed a novel single-step capillary electrophoresis (SSCE) scheme for miniaturized and easy to use system by using a microchannel chip, which was made from the hydrophilic material polymethyl methacrylate (PMMA), equipped with a capillary stop valve. Taking the surface tension property of liquids into consideration, the capillary effect was used to introduce liquids and control capillary stop valves in a partial barrier structure in the wall of the microchannel. Through the combined action of stop valves and air vents, both sample plug formation for electrophoresis and sample injection into a separation channel were successfully performed in a single step. To optimize SSCE, different stop valve structures were evaluated using actual microchannel chips and the finite element method with the level set method. A partial barrier structure at the bottom of the channel functioned efficiently as a stop valve. The stability of stop valve was confirmed by a shock test, which was performed by dropping the microchannel chip to a floor. Sample plug deformation could be reduced by minimizing the size of the side partial barrier. By dissolving hydroxyl ethyl cellulose and using it as the sample solution, the EOF and adsorption of the sample into the PMMA microchannel were successfully reduced. Using this method, a 100-bp DNA ladder was concentrated; good separation was observed within 1 min. At a separation length of 5 mm, the signal was approximately 20-fold higher than a signal of original sample solution by field-amplified sample stacking effect. All operations, including liquid introduction and sample separation, can be completed within 2 min by using the SSCE scheme.

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Portable Simultaneous Multiple Analyte Whole-Blood Analyzer for Point-of-Care Testing

Cited by 50 publications

References 1 publication

Efficient Development of Integrated Lab-On-A-Chip Systems Featuring Operational Robustness and Manufacturability

Efficient Development of Integrated Lab-On-A-Chip Systems Featuring Operational Robustness and Manufacturability

Micro-scale blood plasma separation: from acoustophoresis to egg-beaters

Single‐step CE for miniaturized and easy‐to‐use system

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