Portal Vein Arterialization Increases Hepatocellular Apoptosis and Inhibits Liver Regeneration

Schleimer, Karina; Stippel, Dirk L.; Kasper, Haino Uwe; Prenzel, Klaus L.; Gaudig, C.; Tawadros, Samir; Hoelscher, Arnulf H.; Beckurts, K. T. E.

doi:10.1016/j.jss.2008.01.021

Cited by 17 publications

(17 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the present study, the ALT level increased significantly in both groups in the early postoperative stage, and then declined to the normal level on postoperative day 7. The changes in the ALT level were consistent with those previously described (3,14).…”

Section: Discussionsupporting

confidence: 91%

TNF-α, HGF and TGF-β1 are Involved in Liver Regeneration Following Partial Hepatectomy Using Portal Vein Arterializations

Niu¹,

Dong²,

Zhang³

et al. 2012

Journal of Medical Biochemistry

View full text Add to dashboard Cite

Kratak sadr`aj: Eksperimenti vezani za regeneraciju jetre posle parcijalne hepatektomije pokazali su da TNF-a, HGF i TGF-b1 kao i drugi citokini igraju va`ne uloge u razli~itim faza ma regeneracije jetre, me|utim, uticaj arterijalizacije port ne vene (APV) na ekspresiju ovih citokina tokom regene racije jetre nije jasan. Sprague Dawley pacovi nasumi~no su podeljeni u APV grupu i kontrolne grupe, a krv za detekciju ALT uzeta je pomo}u automatskog biohemijskog analiza tora. Ekspresije TNF-a, HGF i TGF-b1 u tkivima jetre detektovane su kvantitativnom RT-PCR. Nivoi ALT u obe grupe u po~etnom periodu posle operacije bili su zna~ajno vi{i nego pre hirur{kog za hvata i postepeno su se normalizovali posle 7 dana od operacije. Posle 12 h i 24 h od zahvata, ekspresija TNF-a u APV grupi bila je zna~ajno vi{a nego u kontrolnoj grupi (P<0,05), ali posle 7 dana od operacije nije uo~ena zna~ajna razlika izme|u dve grupe. Ekspresija HGF u APV grupi posle 12 h bila je sli~na onoj u kontrolnoj grupi, ali je posle 24 h bila zna~ajno vi{a nego u kontrolnoj grupi (P<0,05). Posle 24 h, ekspresija TGF-b1 u APV grupi bila je zna~ajno ni`a nego u kontrolnoj grupi (P<0,05), ali posle 48 h od operacije izme|u dve grupe nije otkrivena zna~ajna razlika. Pozitivan uticaj na arterija li zaciju portne vene u ranim fazama regeneracije jetre bio je povezan s promenama u ekspresijama TNF-a, HGF i TGF-b1. Summary: Experiments on liver regeneration after partial hepatectomy have shown that TNF-a, HGF and TGF-b1 and other cytokines play important roles in the different stages of liver regeneration, however, the effect of portal vein arteria liz ation (PVA) on the expressions of these cytokines during liver regeneration is not clear. Sprague Dawley rats were randomly divided into the PVA group and control groups, and blood was collected for the detection of ALT using an automatic bio chemical analyzer. The expressions of TNF-a, HGF and TGF-b1 in liver tissues were detected by quan titative RT-PCR. The ALT levels in both groups in the early period after surgery were significantly higher than those before operation, and gradually returned to normal at 7 days after surgery. At 12 h and 24 h after operation, the TNF-a expres sion in the PVA group was significantly higher than that in the control group (P<0.05), but no significant dif fe rence at 7 days after sur gery was observed between the two groups. At 12 h, the HGF expression in the PVA group was similar to that in the con trol group, but significantly higher than in the control group at 24 h (P<0.05). At 24 h, the TGF-b1 expres sion in the PVA group was sig nificantly lower than that in the control group (P <0.05), but no significant difference was found at 48 h after surgery between the two groups. The promo tive effects on the portal vein arterialization at the early stage of liver rege neration were associated with the changes in the expressions of TNF-a, HGF and TGF-b1.

show abstract

Section: Discussionsupporting

confidence: 91%

TNF-α, HGF and TGF-β1 are Involved in Liver Regeneration Following Partial Hepatectomy Using Portal Vein Arterializations

Niu¹,

Dong²,

Zhang³

et al. 2012

Journal of Medical Biochemistry

View full text Add to dashboard Cite

show abstract

“…However, graft survival in their study was low (<50% by 30 postoperative days) and they also ligated the native bile duct; consequently the native liver shrank, similarly to the procedure of Hess et al with a survival rate of only 44.4% 17 . Others have reported portal arterialization 19 producing a survival rate at 14 days of 77.7%; however, in these studies, hepatocytes did not survive long-term with arterial blood flow alone 32,33 . Marni et al 20 also achieved a high survival rate of 80% at 2 weeks and 73.3% at 8 weeks by using a cuff technique for PV and IVC anastomosis.…”

Section: Discussionmentioning

confidence: 71%

Regeneration and Cell Recruitment in an Improved Heterotopic Auxiliary Partial Liver Transplantation Model in the Rat

Perez‐Gutierrez

Yovchev

Murakami³

et al. 2017

Transplantation

View full text Add to dashboard Cite

Background Auxiliary partial liver transplantation (APLT) in humans is a therapeutic modality used especially to treat liver failure in children or congenital metabolic disease. Animal models of APLT have helped to explore therapeutic options. Though many groups have suggested improvements, standardizing the surgical procedure has been challenging. Additionally, the question of whether graft livers are reconstituted by recipient-derived cells after transplantation has been controversial. The aim of this study was to improve experimental APLT in rats and to assess cell recruitment in the liver grafts. Methods To inhibit recipient liver regeneration and to promote graft regeneration, we treated recipients with retrorsine and added arterial anastomosis. Using green fluorescence protein transgenic rats as recipients, we examined liver resident cell recruitment within graft livers by immunofluorescence co-staining. Results In the improved APLT model, we achieved well-regenerated grafts that could maintain regeneration for at least 4 weeks. Regarding the cell recruitment, there was no evidence of recipient-derived hepatocyte, cholangiocyte or hepatic stellate cell recruitment into the graft. Macrophages/monocytes, however, were consistently recruited into the graft and increased over time, which might be related to inflammatory responses. Very few endothelial cells showed co-localization of markers. Conclusions We have successfully established an improved rat APLT model with arterial anastomosis as a standard technique. Using this model, we have characterized cell recruitment into the regenerating grafts.

show abstract

“…The radiological intervention is less invasive than surgical intervention and effective with fewer complications 1) ; however, as Roux et al noted 14) , potential complications such as ischemic change to the liver parenchyma, development of abscess, and coil migration warrant consideration of the risks of endovascular treatment. After treatment, the clinical prognosis of APFs is favorable 1,2,4) ; however, the long-term effect of the APFs may have negative impacts on the regeneration of hepatic parenchyma or the parenchymal structures 15,16) . Thus, we suggest that asymptomatic APFs or remnant fistulas after embolization of large/multiple APFs should be monitored.…”

Section: Discussionmentioning

confidence: 99%

A case of ascites and SMV thrombosis dueto an intrahepatic arterio-portal fistula after hepatectomy

Munekage

Maeda

Munekage

et al. 2019

Ann. Cancer Res. Therap.

View full text Add to dashboard Cite

Arterio-portal fistula (APF) is a rare vascular abnormality, characterized by communication between artery and portal vein. We describe a 69-year-old male patient with APF, who developed acute clinical symptoms 10 months after hepatectomy. The APF was accompanied by massive ascites and thrombosis in the superior mesenteric vein. The condition was successfully treated using anticoagulant, diuretics, and interventional coil embolization of the fistulas. This case indicates that APF is an important postoperative complication after hepatectomy, and that intrahepatic APFs could manifest acutely with ascites and superior mesenteric vein thrombosis.

show abstract

Portal Vein Arterialization Increases Hepatocellular Apoptosis and Inhibits Liver Regeneration

Cited by 17 publications

References 29 publications

TNF-α, HGF and TGF-β1 are Involved in Liver Regeneration Following Partial Hepatectomy Using Portal Vein Arterializations

TNF-α, HGF and TGF-β1 are Involved in Liver Regeneration Following Partial Hepatectomy Using Portal Vein Arterializations

Regeneration and Cell Recruitment in an Improved Heterotopic Auxiliary Partial Liver Transplantation Model in the Rat

A case of ascites and SMV thrombosis dueto an intrahepatic arterio-portal fistula after hepatectomy

Contact Info

Product

Resources

About