POSaM: a fast, flexible, open-source, inkjet oligonucleotide synthesizer and microarrayer

Abstract

POSaM: a fast, flexible, open-source, inkjet oligonucleotide synthesizer and microarrayer <p>DNA arrays are valuable tools in molecular biology laboratories. Their rapid acceptance was aided by the release of plans for a pin-spotting microarrayer by researchers at Stanford. Inkjet microarraying is a flexible, complementary technique that allows the synthesis of arrays of any oligonucleotide sequences <it>de novo</it>. We describe here an open-source inkjet arrayer capable of rapidly producing sets of unique 9,800-feature arrays.</p> Abstract DNA arrays are valuable tools in molecular biology laboratories. Their rapid acceptance was aided by the release of plans for a pin-spotting microarrayer by researchers at Stanford. Inkjet microarraying is a flexible, complementary technique that allows the synthesis of arrays of any oligonucleotide sequences de novo. We describe here an open-source inkjet arrayer capable of rapidly producing sets of unique 9,800-feature arrays.

Synthetic genomics: Options for governance

“…76 There are few externally observable indicators (other than supply of reagents) that would denote the existence or operation of an unregistered synthesizer. Therefore, Option II-3, requiring that materials or maintenance be provided only for synthesizers that are licensed would increase still further the difficulty of operating unregistered machines.…”

Homogeneous Immobilization of Probe DNAs on DNA Chip Using Polyurea Thin Film

“…There are two major fabrication methods of DNA microarrays: the photolithographic synthesis of probes on a chip using light-sensitive masking agents and nucleoside phosphoramidites-based chemistry [3] and the covalent immobilization of prefabricated oligonucleotide probes onto chemically activated surfaces using spotting devices such as ink-jets [4]. Silica (glass or quartz) is commonly used as a microarray substrate due to its chemical resistance, low intrinsic fluorescence, and suitability for silane coupling formation.…”

Airborne Particle Generation Through Acoustic Ejection of Particles-In-Droplets

“…Ink-jet printing has attracted a large interest for applications outside its conventional use in printing text and graphics, especially as a manufacturing technology for fabrication of biochemical microarrays (Lausted et al 2004), 3-D microelectromechanical systems (Fuller et al 2002), and microelectronic components (Hebner et al 1998). Recently, technologies developed for ink-jet printing have also been used to produce aerosol particles for developing and testing instrumentation for studying and monitoring indoor and atmospheric aerosols, including harmful aerosols such as bacteria, pollens, or combustion aerosols; for testing aerosol protection or control equipment (e.g., filters, protective masks, air cleaning systems); and for pharmaceutical dispensing applications (Bottiger et al 1998;Pan et al 2003;Schafer et al 2007;Davitt et al 2005).…”