The evolutionary mechanisms underlying duplicate gene maintenance and divergence remain highly debated. Epigenetic modifications, such as DNA methylation, may contribute to duplicate gene evolution by facilitating tissue-specific regulation. However, the role of epigenetic divergence on duplicate gene evolution remains little understood. Here we show, using comprehensive data across 10 diverse human tissues, that DNA methylation plays critical roles in several aspects of duplicate gene evolution. We first demonstrate that duplicate genes are initially heavily methylated, before gradually losing DNA methylation as they age. Within each pair, DNA methylation divergence between duplicate partners increases with evolutionary age. Importantly, tissuespecific DNA methylation of duplicates correlates with tissuespecific expression, implicating DNA methylation as a causative factor for functional divergence of duplicate genes. These patterns are apparent in promoters but not in gene bodies, in accord with the complex relationship between gene-body DNA methylation and transcription. Remarkably, many duplicate gene pairs exhibit consistent division of DNA methylation across multiple, divergent tissues: For the majority (73%) of duplicate gene pairs, one partner is always hypermethylated compared with the other. This is indicative of a common underlying determinant of DNA methylation. The division of DNA methylation is also consistent with their chromatin accessibility profiles. Moreover, at least two sequence motifs known to interact with the Sp1 transcription factor mark promoters of more hypomethylated duplicate partners. These results demonstrate critical roles of DNA methylation, as well as complex interaction between genome and epigenome, on duplicate gene evolution.gene duplication | tissue specificity | epigenomics | genomics G ene duplication is a main process generating genomic repertoires for subsequent functional innovation (1, 2). Elucidating the mechanisms by which newly duplicated genes are retained and evolve new functions is one of the most fundamental problems in molecular evolution. Even though significant progresses have been made (3-9), detailed molecular mechanisms of duplicate gene divergence are not satisfactorily resolved (10, 11).Here we examine a relatively new avenue of research on duplicate gene evolution: the epigenetic divergence of duplicate genes and how this divergence might relate to functional differentiation of duplicate genes. We focus on DNA methylation. It has been proposed that epigenetic mechanisms such as DNA methylation may facilitate evolution by gene duplication (12). Rodin and Riggs (12) have demonstrated by computational modeling that rates of functional diversification such as subfunctionalization and neofunctionalization increase when epigenetic silencing of duplicates is possible. This advantage of epigenetic silencing is particularly significant when effective population sizes are small, as in humans (12).Some earlier observations are consistent with epigenetic silencing...