Position of anthers at plating and its influence on anther callusing in rice

Mercy, S. T.; Zapata, F. J.

doi:10.1007/bf00272008

Cited by 13 publications

(3 citation statements)

References 4 publications

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“…Effects of orientation in response to in vitro culture have been reported in many plants such as Oryza (Mercy and Zapata, 1987) and Brassica (Arnison et al, 1990). However, there has been one report on the orientation of banana embryos (Asif et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

The Influence of Explant Types and Orientation on in Vitro Culture

Kanchanapoom

Promsorn

2011

Not Sci Biol

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Inflorescence, apical and lateral buds of Musa balbisiana 'Kluai Hin' (BBB group) were used to culture on MS medium supplemented with 22 µM BA and 15% (v/v) coconut water. Comparison of bud orientation showed that the best response of in vitro shoot tip proliferation was obtained with abaxial surface of buds lying down i.e. one side touching the medium (tilt). Mass propagation of shoot tips was obtained when cultured buds on MS medium containing 44 µM BA. Rooting was achieved by transferring the isolated shoots to MS basal medium without growth regulators. Rooted plantlets were acclimatized and successfully established in soil.

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Section: Discussionmentioning

confidence: 99%

The Influence of Explant Types and Orientation on in Vitro Culture

Kanchanapoom

Promsorn

2011

Not Sci Biol

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“…However, the androgenic response can also be influenced by factors like the spike position on the tiller [14][15][16] and the spikelet position on the spike [5,17,18]. Furthermore, the yield of androgenesis using in vitro culture of anthers was shown to depend on anther orientation on the culture medium [19][20][21][22][23]. Despite the known influence of these factors, often they are not taken into account during in vitro culture of anthers and their role remains poorly studied.…”

Section: Introductionmentioning

confidence: 99%

Effect of Cold Pretreatment, Anthers Orientation, Spikelet Position and Donor Tiller on the Callusing Response in Barley Anther In vitro Culture

S¹

2017

IJMBG

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The anthers in vitro culture is a useful technique for plant improvement as to create new varieties. However, the success of this technique is limited by several factors. This study was carried out to identify factors as the influence of spikelet position in the spike, position of donor tiller on plate tillering and anther orientation onto the medium as well as cold pretreatment and the genotype that affect the callusing induction in anthers in vitro culture of three cultivars of Moroccan barley (Arig 8, Asni and Tamelalt). Among genotypes, the callusing response was significantly higher in Asni (21.42%). The callusing response was significantly decreased when spikes stemmed from tillers far from the main tiller on the same tillering plate. However, anthers coming from the spikelets of the middle part of the spike showed the highest callusing response (29.85%) compared with the basal (14.83%) and the upper (6.72%) parts. It was also shown that anther cold pretreatment at 4°C for 14 days had a beneficial effect only on the callusing response but it decreased the regeneration of green plants, due to the interaction between cold pretreatment and genotypes to which spring barley is recalcitrant and more predisposed to albinism, especially six-row type (Arig 8). Moreover, anther orientation onto the surface medium had a marked effect on callusing response, anthers placed in upper orientation developed more calli (60.22%) than those in flat position (18.99%). Our findings suggest that the studied factors control the callusing response. Especially, the Anthers orientation that must be taken into account during androgenesis through anthers in vitro culture.

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“…Orientation of explants on solid media has been shown to influence the developmental pathway of explant tissues or organs in other species. For instance, anthers of rice (Mercy & Zapata 1987), and barley (Shannon et al 1985), plated edge-wise with only one lobe in contact with medium, produced more embryoids capable of plantlet differentiation than those plated fiat with both lobes in contact with medium.…”

Section: Introductionmentioning

confidence: 99%