Positive and negative fast atom bombardment mass spectrometry and collision spectroscopy in the structural characterization of mono-, di- and triglycerides

Abstract

The structural characterization of a selection of mono-, di-and triglycerides has been achieved by means of positive and negative ion fast atom bombardment (FAB) mass spectrometry combined with collision experimenh While

One‐step isolation of γ‐oryzanol from rice bran oil by non‐aqueous hydrostatic countercurrent chromatography

“…5) 4 , error 1.11 ppm, RDB 4) if no attention is paid to molecular formula afforded by mass accuracy. Some widespread di-and triglycerides lead to these fragments at m/z 603, 577 and 339, corresponding either to the loss of water, oleoyl, palmitoyl or stearoyl moieties [33]. As it is illustrated in Fig.…”

Applications and mechanisms of charge-remote fragmentation

“…Although all these techniques can provide the molecular weight of the intact molecule and some information about the acyl groups, it has not been possible to perform a complete structural determination with mass spectrometry alone. Evans et al (1991) observed that abundant [M Na À R n COOH] and other product ions are formed in the fragmentation of FAB-produced [M Na] ions of triacylglycerols. Some product ions are likely formed by charge-remote fragmentation, although no spectra were shown in their article.…”

Structural elucidation of co‐eluted triglycerides in the marine diatom model organism Thalassiosira pseudonana by ultra‐performance liquid chromatography/quadrupole time‐of‐flight mass spectrometry

“…These methods are time‐consuming and cannot confirm the acyl position in TAGs . Mass spectrometry using a variety of ionization techniques has been applied for the characterization of TAGs, including electron impact, chemical ionization, field desorption, desorption chemical ionization, fast atom bombardment (FAB), thermospray, electrospray ionization (ESI), and atmospheric pressure chemical ionization (APCI) . Recently, although structural characterization of regiospecific TAGs by ESI 3 in vegetable oils has been reported, a multilevel MS analysis for completing regiospecific TAGs is still desired .…”