Structural elucidation of co‐eluted triglycerides in the marine diatom model organism <i>Thalassiosira pseudonana</i> by ultra‐performance liquid chromatography/quadrupole time‐of‐flight mass spectrometry

Li, Shuang; Xu, Jilin; Chen, Jiao; Chen, Juanjuan; Zhou, Chengxu; Yan, Xiaojun

doi:10.1002/rcm.6784

Cited by 11 publications

(10 citation statements)

References 40 publications

(83 reference statements)

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“…found that under different collision energy (5–80 V), the abundance of ions [M + Na–R 1 COOH] + of MGDG and DGDG was always greater than that of ions [M + Na–R 2 COOH] + , respectively. The same observation was made by Li et al . that [M + Li–R 1/3 COOH] + of TAG had greater abundance than that of [M + Li–R 2 COOH] + using various collision energies (6–80 V).…”

Section: Resultscontrasting

confidence: 56%

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Simultaneous structural identification of diacylglyceryl‐N‐trimethylhomoserine (DGTS) and diacylglycerylhydroxymethyl‐N,N,N‐trimethyl‐β‐alanine (DGTA) in microalgae using dual Li⁺/H⁺ adduct ion mode by ultra‐performance liquid chromatography/quadrupole time‐of‐flight mass spectrometry

Lou

Mu³

et al. 2017

Rapid Comm Mass Spectrometry

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Section: Resultscontrasting

confidence: 56%

“…Li et al . utilized [M + Li] + precursor ions for structural elucidation of triglycerides (TAGs) . Zhao et al .…”

Section: Resultsmentioning

confidence: 99%

Simultaneous structural identification of diacylglyceryl‐N‐trimethylhomoserine (DGTS) and diacylglycerylhydroxymethyl‐N,N,N‐trimethyl‐β‐alanine (DGTA) in microalgae using dual Li⁺/H⁺ adduct ion mode by ultra‐performance liquid chromatography/quadrupole time‐of‐flight mass spectrometry

Lou

Mu³

et al. 2017

Rapid Comm Mass Spectrometry

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“…In addition to the polar lipids, recent studies have shown that different microalgal species contain a diverse array of TAGs and these TAGs can also be used for rapidly distinguishing microalgae (Li et al. ,b). There were 12TAGs in NMBluh014 and 16 TAGs in NMBluh‐identified.…”

Section: Resultsmentioning

confidence: 99%

“…), but recent studies have shown that different microalgal species contain a diverse composition of lipids, and that these lipids can be used to rapidily distinguish microalgae (Li et al. ,b). Hence, the qualitative and semiquantitative analyses of lipids have been completed separately.…”

Section: Methodsmentioning

confidence: 99%

Lipidomic analysis can distinguish between two morphologically similar strains of Nannochloropsis oceanica

Jiang

et al. 2015

Journal of Phycology

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The two morphologically similar microalgae NMBluh014 and NMBluh-X belong to two different strains of Nannochloropsis oceanica. They possess obviously different feeding effects on bivalves, but are indistinguishable by 18S rRNA and morphological features. In this work, lipidomic analysis followed by principal component analysis and orthogonal projections to latent structures discriminant analysis provided a clear distinction between these strains. Metabolites that definitively contribute to the classification were selected as potential biomarkers. The most important difference in polar lipids were sulfoquinovosyldiacylglycerol (containing 18:1/16:0 and 18:3/16:0) and monogalactosyldiacylglycerol (containing 18:3/16:3 and 20:5/14:0), which were detected only in NMBluh-X. Additionally, an exhaustive qualitative and quantitative profiling of the neutral lipid triacylglycerol (TAG) in the two strains was carried out. The predominant species of TAG containing 16:1/16:1/16:1 acyl groups was detected only in NMBluh-X with a content of ~93.67 ± 11.85 nmol · mg(-1) dry algae at the onset of stationary phase. Meanwhile, TAG containing 16:0/16:0/16:0 was the main TAG in NMBluh014 with a content of 40.25 ± 3.92 nmol · mg(-1) . These results provided the most straightforward evidence for differentiating the two species. The metabolomic profiling indicated that NMBluh-X underwent significant chemical and physiological changes during the growth process, whereas NMBluh014 did not show such noticeable time-dependent metabolite change. This study is the first using Ultra Performance Liquid Chromatography coupled with Electrospray ionization-Quadrupole-Time of Flight Mass Spectrometry (UPLC-Q-TOF-MS) for lipidomic profiling with multivariate statistical analysis to explore lipidomic differences of plesiomorphous microalgae. Our results demonstrate that lipidomic profiling is a valid chemotaxonomic tool in the study of microalgal systematics.

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“…Liquid chromatography-mass spectrometry has been widely used for the analysis of various lipid classes [16,17]. A variety of mass spectrum techniques is available to determine the exact TAG structure of SCO [18][19][20]. The use of tandem mass spectrometric (MS/MS) analysis is crucial to identify TAG present in the lipid.…”

Section: Introductionmentioning

confidence: 99%

Composition and Structure of Single Cell Oil Produced by Schizochytrium limacinum SR31

Wang

Lou

Tao

et al. 2016

J Americ Oil Chem Soc

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Schizochytrium limacinum sp. was commercially utilized to produce single cell oil (SCO), but its detailed lipid profile remains unknown. In order to analyze the composition and structure of SCO produced by Schizochytrium limacinum SR31, the SCO extracted at stationary growth phase was separated into neutral lipids (NL), polar lipids and glycolipids by using silica gel column chromatography. The result of lipid classes in NL revealed that triacylglycerol (TAG) was the primary glyceride (93.81 %). Fatty acid (FA) analysis by gas chromatography-mass spectrometer (GC-MS) proved that palmitic acid (29.78 %) and docosahexaenoic acid (25.64 %) were the major FA. Quadrupole time of flight mass spectrometry in electrospray ionization mode coupled with ultra-performance liquid chromatography was carried out to investigate TAG structures. More than 80 species were identified. Positional distribution of FA in TAG might contribute to further study of the FA shifts during the lipid turnover stage and nutritional evaluation of the SCO.

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Structural elucidation of co‐eluted triglycerides in the marine diatom model organism Thalassiosira pseudonana by ultra‐performance liquid chromatography/quadrupole time‐of‐flight mass spectrometry

Cited by 11 publications

References 40 publications

Lipidomic analysis can distinguish between two morphologically similar strains of Nannochloropsis oceanica

Composition and Structure of Single Cell Oil Produced by Schizochytrium limacinum SR31

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