Positive and negative phenotyping of increasing Zn concentrations by Biospeckle Optical Coherence Tomography in speedy monitoring on lentil (Lens culinaris) seed germination and seedling growth

Silva, Y. Sanath K. De; Rajagopalan, Uma Maheswari; Kadono, Hirofumi; Li, Danyang

doi:10.1016/j.stress.2021.100041

Cited by 19 publications

(18 citation statements)

References 57 publications

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“…In our previous study, the positive and negative phenotyping of increasing Zn concentrations (5, 10, and 100 mg L -1 ) on lentil seed germination and seedling growth were investigated using bOCT. The results imply a positive effect for 5 and 10 mg L -1 whereas 100 mg L -1 Zn exhibits a strong toxic effect on lentils 8 . Due to the widespread usage and mismanagement, increasing MPs and heavy metal accumulation have been observed in agricultural land leading to the combined toxicity.…”

Section: Introductionmentioning

confidence: 80%

Biospeckle optical coherence tomography reveals the mitigation of the harmful effects of heavy metal zinc in combination with polyethylene microplastics in lentil seeds

Silva¹,

Rajagopalan²,

Kadono³

2023

SPIE Future Sensing Technologies 2023

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Microplastic pollution has become prevalent due to industrialization and the wide use of plastic. In this study, the individual and combined effects of polyethylene microplastic with Zn on lentil seed germination were monitored using Biospeckle Optical Coherence Tomography (bOCT), a technique established by authors to visualize the internal activity of biological objects. The results imply that both 100 mg L-1 heavy metal Zn or polyethylene microplastics (PEMPs) alone had a strong inhibitory effect on lentils. However, in combination with PEMPs, the Zn toxicity on lentils was reduced due to the reduction of Zn uptake by the seedlings through the interaction of Zn and MPs. These findings suggest that PEMPs could be able to mitigate the effect on the bioavailability and toxicity of Zn on lentils.

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Section: Introductionmentioning

confidence: 80%

Biospeckle optical coherence tomography reveals the mitigation of the harmful effects of heavy metal zinc in combination with polyethylene microplastics in lentil seeds

Silva¹,

Rajagopalan²,

Kadono³

2023

SPIE Future Sensing Technologies 2023

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“…The intensity of the biospeckle changes randomly according to the movement of organelles within the biological tissue. By investigating the dynamic changes of the biospeckles, we can learn about the activities within the biological tissue [30] . Figure 5 shows a typical biospeckle speckle image taken from the leaf of a 3-month old plant.…”

Section: Laser Biospeckle and Evaluationmentioning

confidence: 99%

“…In contrast, laser biospeckle method is a technology that enables nondestructive and non-contact evaluation of the structure of a biological objects [25][26] . In the agricultural field, it has been used to evaluate the quality of fruits and vegetables [27][28][29] , but few studies have focused on the plant growth evaluation and environment [30] . In fact, Hirai et al demonstrated that the response of plants to different sound frequencies could be rapidly evaluated by laser biospeckle [31] , indicating the potential of laser biospeckles in plant monitoring studies.…”

Section: Introductionmentioning

confidence: 99%

Laser biospeckle based novel method in the evaluation of far-red light effects on plant growth

Igarashi

Takemura

Kono

et al. 2023

Optical Interactions With Tissue and Cells XXXIV

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While artificial cultivation is gaining prominence due to rapid climate change, lighting costs remain a challenge. Therefore, research is needed to cultivate plants more efficiently. At the same time, it has been found that a mixture of far-red light (FR) and red light (R) also promotes growth through the action of phytochrome, a photoreceptor in the plant body. However, these studies require time and damaging of the plant to measure the dry mass and area of leaves, and immediate effects have not been investigated so far. Therefore, in this study, we propose laser biospeckle to evaluate the relationship between plant growth duration and FR response. Laser biospeckles are formed by light scattered from organelles in plant tissues by laser irradiation. The intensity of these speckles changes with time, and by examining these changes, the activity inside the plant can be evaluated. Biospeckles of arugula leaves were obtained by irradiating the leaves with laser light of wavelength 852 nm and simultaneously with LED light of wavelength 735 nm (FR) and 630 nm (R). Biospeckle movies under FR and non-irradiation were recorded by a CMOS camera at 15 frames/sec for 20 seconds. From the movie, correlation between the first frame and the subsequent frames were calculated. Experimental results showed that arugula at 1-month after seed planting showed an immediate FR response, while those at 3-month showed a sustained response. The relationship between biospeckle movement and plant growth behaviour is under investigation.

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“…The applicability of OCT to study internal morphological variations in seeds has been demonstrated in several studies, such as measurements of hull thickness in lupin seeds [ 16 ], the in situ monitoring of different phases of germination in pea seeds [ 17 ], the assessment of morphological modifications induced by viral infections in melon seeds [ 18 ], distinguishing between healthy and virus-infected cucumber seeds [ 19 ], 3-dimensional imaging of the internal structure of tomato seeds [ 20 ], and the identification of fungus-infected tomato seeds [ 21 ]. A swept-source OCT setup combined with deep neural network approaches was used for the nondestructive classification of rice grains [ 22 ], and a technique dubbed biospeckle OCT [ 23 ] was used to study the phenotypical effects of increasing zinc concentrations on lentil seed germination and seedling growth. Pericarp thickness measurements reported by Clements et al [ 16 ] were based on an OCT system providing an estimated lateral resolution of 30 µm and an axial resolution of 24 µm.…”

Section: Introductionmentioning

confidence: 99%

Non-Destructive Direct Pericarp Thickness Measurement of Sorghum Kernels Using Extended-Focus Optical Coherence Microscopy

Sen

Fernández

Crozier

et al. 2023

Sensors

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Non-destructive measurements of internal morphological structures in plant materials such as seeds are of high interest in agricultural research. The estimation of pericarp thickness is important to understand the grain quality and storage stability of seeds and can play a crucial role in improving crop yield. In this study, we demonstrate the applicability of fiber-based Bessel beam Fourier domain (FD) optical coherence microscopy (OCM) with a nearly constant high lateral resolution maintained at over ~400 µm for direct non-invasive measurement of the pericarp thickness of two different sorghum genotypes. Whereas measurements based on axial profiles need additional knowledge of the pericarp refractive index, en-face views allow for direct distance measurements. We directly determine pericarp thickness from lateral sections with a 3 µm resolution by taking the width of the signal corresponding to the pericarp at the 1/e threshold. These measurements enable differentiation of the two genotypes with 100% accuracy. We find that trading image resolution for acquisition speed and view size reduces the classification accuracy. Average pericarp thicknesses of 74 µm (thick phenotype) and 43 µm (thin phenotype) are obtained from high-resolution lateral sections, and are in good agreement with previously reported measurements of the same genotypes. Extracting the morphological features of plant seeds using Bessel beam FD-OCM is expected to provide valuable information to the food processing industry and plant breeding programs.

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Positive and negative phenotyping of increasing Zn concentrations by Biospeckle Optical Coherence Tomography in speedy monitoring on lentil (Lens culinaris) seed germination and seedling growth

Cited by 19 publications

References 57 publications

Biospeckle optical coherence tomography reveals the mitigation of the harmful effects of heavy metal zinc in combination with polyethylene microplastics in lentil seeds

Biospeckle optical coherence tomography reveals the mitigation of the harmful effects of heavy metal zinc in combination with polyethylene microplastics in lentil seeds

Laser biospeckle based novel method in the evaluation of far-red light effects on plant growth

Non-Destructive Direct Pericarp Thickness Measurement of Sorghum Kernels Using Extended-Focus Optical Coherence Microscopy

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