Positive growth of smooth muscle in uterine horns of myostatin homozygous mutant gilt

Liu, Xinyue; Choe, Hak Myong; Li, Zhou-Yan; Jin, Zheng-Yun; Chang, Shuangyan; Kang, Jin‐Dan; Yin, Xi-Jun; Quan, Biao-Hu

doi:10.1016/j.rvsc.2022.07.030

Cited by 4 publications

(9 citation statements)

References 34 publications

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Section: Cross-validation Of Cluster Assignment Through Region-specif...supporting

confidence: 64%

“…AQP3 was observed in the epithelial zone, consistent with its known role as an epithelium marker [44]. Similarly, ACTA2[45] and Desmin (DES) [46] in the muscularis propria confirmed their utility in identifying smooth muscle cells. Additional markers, including ISL1 for vaginal mesenchyme [6], LYVE1 for lymphatic vessels [47], Tp63 for basal region cells of the vaginal epithelium [6], and F11R for the parabasal and basal layers of the epithelium [8], were observed in corresponding locations with related patterns (Figure 4A and Figure S4A).…”

Section: Resultsmentioning

confidence: 64%

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Molecular stratification of human fetal vaginal epithelium by spatial transcriptome analysis

Ye,

Jiang,

Zhu

et al. 2024

Preprint

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The human vaginal epithelium is a crucial component in numerous reproductive processes and serves as a vital protective barrier against pathogenic invasion. Despite its significance, a comprehensive exploration of its molecular profiles, including molecule expression and distribution across its multiple layers, remains elusive. In our study, we undertook a spatial transcriptomic analysis within the vaginal wall of human fetuses to fill this knowledge gap. We successfully categorized vaginal epithelium into four distinct zones based on their transcriptomic profiles and anatomical features. This approach unveiled unique transcriptomic signatures within these regions, allowing us to identify differentially expressed genes and uncover novel markers for distinct regions of the vaginal epithelium. Additionally, our findings have highlighted the varied expression of KRT genes across different zone of the vaginal epithelium, with a gradual shift in expression patterns observed from the basal layer to the surface/superficial layer. This suggests a potential differentiation trajectory of human vaginal epithelium, shedding light on the dynamic nature of this tissue. Furthermore, abundant biological processes were found to be enriched in the basal zone by the KEGG pathway analysis, indicating an active state of the basal zone cells. Subsequently, the expression of latent stem cell markers in the basal zone were identified. In summary, our research provides crucial understanding of human vaginal epithelial cells and the complex mechanisms of the vaginal mucosa, with potential applications in vaginal reconstruction and drug delivery, making this atlas a valuable tool for future research in women's health and reproductive medicine.

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Section: Cross-validation Of Cluster Assignment Through Region-specif...supporting

confidence: 64%

Section: Resultsmentioning

confidence: 64%

Molecular stratification of human fetal vaginal epithelium by spatial transcriptome analysis

Ye,

Jiang,

Zhu

et al. 2024

Preprint

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“…RNAi was applied to investigate the porcine MSTN gene. − However, to the best of our knowledge, no MSTN knockdown pigs were generated using this approach. ZNFs were applied to target the porcine MSTN in somatic cells , and to generate live ZFNs-mediated MSTN -edited pigs − (Table ).…”

Section: Targeting the Mstn Gene In Pigsmentioning

confidence: 99%

“…One of these MSTN -edited models, its progeny, or its crosses were further investigated . These include investigations on fiber-type distribution and expression of myosin heavy chain isoforms; shift from slow- to fast-twitch muscle fibers in skeletal muscle; internal organ size; semen quality and fertilization ability; reproductive trait characterization in MSTN -edited crosses; collagen-related pathological features (umbilical hernia and tippy toe standing); regulation of body fat deposition; fibrinogen levels and fibrin clot structure; esophageal striated muscle development and regulation of muscle fiber types; extraocular muscles; cardiac extracellular matrix; growth of smooth muscle in uterine horns of MSTN mutant gilts; smooth muscle content in corpus cavernosum of MSTN mutant boars; erythrocyte osmotic fragility, hematological parameters, and fatty acid composition of serum and erythrocyte membranes; miR-455-3p regulation of myoblast differentiation; composition and alteration of gut microbiota; analysis of meat and fatty acids characteristics; and development of a restriction enzyme-mediated PCR-RFLP assay for genotyping MSTN mutant pigs …”

Section: Targeting the Mstn Gene In Pigsmentioning

confidence: 99%

When Less Is More: Targeting the Myostatin Gene in Livestock for Augmenting Meat Production

Kalds

Zhou

Huang

et al. 2023

J. Agric. Food Chem.

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How to increase meat production is one of the main questions in animal breeding. Selection for improved body weight has been made and, due to recent genomic advances, naturally occurring variants that are responsible for controlling economically relevant phenotypes have been revealed. The myostatin (MSTN) gene, a superstar gene in animal breeding, was discovered as a negative controller of muscle mass. In some livestock species, natural mutations in the MSTN gene could generate the agriculturally desirable double-muscling phenotype. However, some other livestock species or breeds lack these desirable variants. Genetic modification, particularly gene editing, offers an unprecedented opportunity to induce or mimic naturally occurring mutations in livestock genomes. To date, various MSTN-edited livestock species have been generated using different gene modification tools. These MSTN gene-edited models have higher growth rates and increased muscle mass, suggesting the high potential of utilizing MSTN gene editing in animal breeding. Additionally, post-editing investigations in most livestock species support the favorable influence of targeting the MSTN gene on meat quantity and quality. In this Review, we provide a collective discussion on targeting the MSTN gene in livestock to further encourage its utilization opportunities. It is expected that, shortly, MSTN gene-edited livestock will be commercialized, and MSTN-edited meat will be on the tables of ordinary customers.

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“…In addition to skeletal muscles, MSTN also exists in smooth muscles [17,18]. To date, a large number of MSTN studies have focused on skeletal muscle [1,19,20], but only a few have focused on smooth muscle [21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Combined Transcriptome and Metabolome Analysis of Smooth Muscle of Myostatin Knockout Cattle

Wang

et al. 2023

IJMS

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Myostatin (MSTN), a growth and differentiation factor, plays an important role in regulating skeletal muscle growth and development. MSTN knockout (MSTN-KO) leads to skeletal muscle hypertrophy and regulates metabolic homeostasis. Moreover, MSTN is also detected in smooth muscle. However, the effect of MSTN-KO on smooth muscle has not yet been reported. In this study, combined metabolome and transcriptome analyses were performed to investigate the metabolic and transcriptional profiling in esophageal smooth muscles of MSTN-KO Chinese Luxi Yellow cattle (n = 5, 24 months, average body weight 608.5 ± 17.62 kg) and wild-type (WT) Chinese Luxi Yellow cattle (n = 5, 24 months, average body weight 528.25 ± 11.03 kg). The transcriptome was sequenced using the Illumina Novaseq™ 6000 sequence platform. In total, 337 significantly up- and 129 significantly down-regulated genes were detected in the MSTN-KO cattle compared with the WT Chinese Luxi Yellow cattle. Functional enrichment analysis indicated that the DEGs were mainly enriched in 67 signaling pathways, including cell adhesion molecules, tight junction, and the cGMP-PKG signaling pathway. Metabolomics analysis by liquid chromatography-tandem mass spectrometry (LC-MS/MS) identified 130 differential metabolites between the groups, with 56 up-regulated and 74 down-regulated in MSTN knockout cattle compared with WT cattle. Differential metabolites were significantly enriched in 31 pathways, including glycerophospholipid metabolism, histidine metabolism, glutathione metabolism, and purine metabolism. Transcriptome and metabolome were combined to analyze the significant enrichment pathways, and there were three metabolically related pathways, including histidine metabolism, purine metabolism, and arginine and proline metabolism. These results provide important references for in-depth research on the effect of MSTN knockout on smooth muscle.

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Positive growth of smooth muscle in uterine horns of myostatin homozygous mutant gilt

Cited by 4 publications

References 34 publications

Molecular stratification of human fetal vaginal epithelium by spatial transcriptome analysis

Molecular stratification of human fetal vaginal epithelium by spatial transcriptome analysis

When Less Is More: Targeting the Myostatin Gene in Livestock for Augmenting Meat Production

Combined Transcriptome and Metabolome Analysis of Smooth Muscle of Myostatin Knockout Cattle

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