Possibilities of Manufacturing an Electrospun Web with Baltic Amber

Milašius, Rimvydas; Ragaišienė, Audronė; Rukuižienė, Žaneta; Mikučionienė, Daiva

doi:10.5604/12303666.1161755

Cited by 3 publications

(3 citation statements)

References 7 publications

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“…with the fluid polymer. The latter, also called needleless technology, usually has a higher production performance and can often be scaled up from laboratory to industrial scale [24][25][26].…”

Section: Electrospinningmentioning

confidence: 99%

Electrospun Nanofibrous Membranes for Tissue Engineering and Cell Growth

Tanzli

Ehrmann

2021

Applied Sciences

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In biotechnology, the field of cell cultivation is highly relevant. Cultivated cells can be used, for example, for the development of biopharmaceuticals and in tissue engineering. Commonly, mammalian cells are grown in bioreactors, T-flasks, well plates, etc., without a specific substrate. Nanofibrous mats, however, have been reported to promote cell growth, adhesion, and proliferation. Here, we give an overview of the different attempts at cultivating mammalian cells on electrospun nanofiber mats for biotechnological and biomedical purposes. Starting with a brief overview of the different electrospinning methods, resulting in random or defined fiber orientations in the nanofiber mats, we describe the typical materials used in cell growth applications in biotechnology and tissue engineering. The influence of using different surface morphologies and polymers or polymer blends on the possible application of such nanofiber mats for tissue engineering and other biotechnological applications is discussed. Polymer blends, in particular, can often be used to reach the required combination of mechanical and biological properties, making such nanofiber mats highly suitable for tissue engineering and other biotechnological or biomedical cell growth applications.

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Section: Electrospinningmentioning

confidence: 99%

Electrospun Nanofibrous Membranes for Tissue Engineering and Cell Growth

Tanzli

Ehrmann

2021

Applied Sciences

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“…Criteria the same as above. Again, the fine divisions in spectral class are important, because if they are off, the luminosity class Milašius et al (2013). The fits files of the spectra are available in the electronic edition of AJ.…”

Section: Seven-color Photometry and Classificationmentioning

confidence: 99%

The Enigma of the Open Cluster M29 (Ngc 6913) Solved

Straižys¹,

Milašius²,

Boyle³

et al. 2014

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Determining the distance to the open cluster M29 (NGC 6913) has proven difficult, with distances determined by various authors differing by a factor of two or more. To solve this problem, we have initiated a new photometric investigation of the cluster in the Vilnius sevencolor photometric system supplementing it with available data in the BV and JHK s photometric systems, and spectra of the nine brightest stars of spectral classes O and B. Photometric spectral classes and luminosities of 260 stars in a 15 ′ × 15 ′ area down to V = 19 mag are used to investigate the interstellar extinction run with distance and to estimate the distance of the Great Cygnus Rift, ∼ 800 pc. The interstellar reddening law in the optical and near-infrared regions is found to be close to normal, with the ratio of extinction to color excess R BV = 2.87. The extinction A V of cluster members is between 2.5 to 3.8 mag, with a mean value of 2.97 mag or E B−V = 1.03. The average distance of eight stars of spectral types O9-B2 is 1.54 ± 0.15 kpc. Two stars from the seven brightest ones are field stars: HDE 229238 is a background B0.5 supergiant and HD 194378 is a foreground F star. In the intrinsic color-magnitude diagram, seven fainter stars of spectral classes B3-B8 are identified as possible members of the cluster. The 15 selected members of the cluster of spectral classes O9-B8 plotted on the log L/L ⊙ vs. log T eff diagram, together with the isochrones from the Padova database, give the age of the cluster as 5 ± 1 Myr.

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“…Nanofi brous mats with the fi bre diameters in the range of some ten to several hundred nanometres can be prepared with electrospinning from diverse polymer solutions or melts. While the easiest technology uses a syringe to extrude the polymer solution through a fi ne needle into a high electric fi eld [1,2], needleless technologies allow for higher production output and can oft en be upscaled from lab to industrial scale [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Sterilization of PAN/Gelatine Nanofibrous Mats for Cell Growth

Wehlage¹,

Blattner²,

Sabantina³

et al. 2019

TEK

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Nanofi brous mats can be used as a substrate for eukaryotic cell growth in biotechnology, tissue engineering, etc. Several adherent cells (e.g. human fi broblasts) have been shown to grow well on fi ne fi bres. For most applications, it is necessary to sterilize nanofi brous mats before adding the cells. Another possibility would be the addition of antibiotics and antimycotics to the cell culture medium to prevent microbial infection. However, antibiotics are disadvantageous since they might promote the growth of resistant bacteria in possible future medical applications of nanofi brous mats. Possible sterilization techniques include autoclaving, UV-sterilization, ozone treatment, heat sterilization and other techniques which usually necessitate more expensive equipment, such as gamma irradiation. Systematic examinations of the infl uence of diff erent sterilization techniques on the cell growth on nanofi brous mats have not yet been reported in the literature. Here, we report on the fi rst experimental investigations of the eff ect of sterilization with diff erent methods on the properties of polyacrylonitrile (PAN)/gelatine nanofi brous mats, and the resulting growth and adhesion of Chinese hamster ovary cells. While all techniques under investigation yielded sterile nanofibrous mats, autoclaving and heat sterilization change the PAN/gelatine fi bre morphology. Ozone, on the other hand, modifi es the pH value of the culture medium and partly impedes cell adhesion. UV sterilization also suggests a chemical modifi cation of the nanofi brous mat. Unexpectedly, heat sterilization resulted in the highest amount of adherent Chinese hamster ovary cells grown on PAN/gelatine nanofi brous mats in spite of gelatine melting.

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Possibilities of Manufacturing an Electrospun Web with Baltic Amber

Cited by 3 publications

References 7 publications

Electrospun Nanofibrous Membranes for Tissue Engineering and Cell Growth

Electrospun Nanofibrous Membranes for Tissue Engineering and Cell Growth

The Enigma of the Open Cluster M29 (Ngc 6913) Solved

Sterilization of PAN/Gelatine Nanofibrous Mats for Cell Growth

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