Possibility to Improve Preservation of Whole Blood by Leukocyte-Depletion before Storage

Riedner, C.; Heim, M.U.; Mempel, W.; Wilmanns, W.

doi:10.1159/000461171

Cited by 10 publications

(9 citation statements)

References 12 publications

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“…However, PF-4 was increased in plasma after filtration of whole blood, possibly indicating that some platelets un derwent a release reaction during filtration. Riedner et al [19] observed a significant increase in ß-thromboglobulin (ß-TG) after filtration, and interpreted this as the result of filtration-induced mechanical damage of platelets. This phenomenon requires further studies, especially since some leukocyte depletion filters adsorb PF-4, ß-TG and other pro teins, such as C3a and C4a [20].…”

Section: Resultsmentioning

confidence: 99%

Prestorage Leukocyte Depletion with In-Line Filtration of Whole Blood in Comparison with Blood Component Leukocyte Depletion

Riggert¹,

Simson²,

Dittmann³

et al. 1995

Vox Sanguinis

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In-line filtration of blood components appears to be an effective method to reduce white-cell-induced adverse reactions. We have investigated whether whole blood filtration (WBF), prior to component preparation, is comparable with filtration of already prepared blood components (CF), i.e. the red cell concentrate (RCC) and fresh plasma. Conventionally prepared nonfiltered blood components served as a control. No significant differences for most parameters investigated were found between leukodepleted RCCs and plasma units prepared by CF or WBF. All filtered RCCs and plasma units (CF and WBF) had white blood cell contaminations < 1 x 10^5 per unit. Platelets were reduced in all filtered components: 95% in plasma and 99% in RCCs. Fresh-frozen plasma (FFP) prepared by CF and WBF had normal amounts of factors V, VIII, von Willebrand factor and thrombin-antithrombin-III complexes, whereas platelet factor 4 (PF-4) was slightly increased in FFP prepared by WBF. RCCs and plasma units prepared from filtered whole blood (n = 20) had a significantly greater volume (RCC: 288+19 ml; plasma: 274±20 ml) than conventionally prepared (n = 20) and filtered products (RCC: 257±19ml, plasma: 259±19ml). For early filtration of blood components, WBF prior to component preparation seems to offer an interesting technique for obtaining a leukocyte-depleted RCC and FFP.

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Section: Resultsmentioning

confidence: 99%

Prestorage Leukocyte Depletion with In-Line Filtration of Whole Blood in Comparison with Blood Component Leukocyte Depletion

Riggert¹,

Simson²,

Dittmann³

et al. 1995

Vox Sanguinis

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“…This could possibly reflect the release of fibrinogen from platelets during filtration. Other studies have shown release of other platelet α‐granule proteins, such as β‐thromboglobulin, owing to whole blood filtration (Reidner et al , 1990). The release of FV from platelet α‐granules may also explain the increase in plasma FV activity observed with the Terumo filter.…”

Section: Discussionmentioning

confidence: 99%

The effect of leucocyte depletion on the quality of fresh‐frozen plasma

et al. 2001

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Summary. The aim of this study was to evaluate the quality of leucodepleted (LD) fresh-frozen plasma (FFP) produced using one of five whole blood filters (Baxter RS2000 & RZ2000, NPBI T2926, Macopharma LST1 and Terumo WBSP) or two plasma filters (Pall LPS1 and Baxter FGR7014). Whole blood or plasma was filtered within 8 h of collection at an ambient temperature. Samples were taken pre-and post filtration for analysis of coagulation factors and complement activation (n 7±12 for each type of filter). All filtered units (209±286 ml) contained , 5 Â 10 6 residual leucocytes and , 30 Â 10 9 /l platelets. Statistically significant losses of factors V, VIII, IX, XI and XII and increases in markers of coagulation activation were observed (0±21%), which were dependent on filter type. None of the filters had a significant effect on von Willebrand factor (VWF) multimeric distribution or the activity of VWF and factors II, VII or X. The effect on levels of C3a appeared to be related to the filter surface charge: positively charged filters resulted in C3a generation, whereas negatively charged resulted in C3a removal. None of the observed changes are likely to be clinically significant unless subsequent processing of plasma (such as pathogen inactivation) results in further losses of coagulation factors.

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“…These leukocyte-quires that blood bags and filter be centrifuged, which may lead to reduced centrifuge capacity and require accurate cup packing. Whole blood filtration may well improve the RBCC storage parameters [18] whilst maintaining plasma coagulation factors [18,19].…”

Section: Methodsmentioning

confidence: 99%

Prestorage Leukocyte Reduction with In-Line Filtration of Whole Blood: Evaluation of Red Cells and Plasma Storage

Rapaille¹,

Moore²,

Flament³

et al. 1997

Vox Sanguinis

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Objectives: Prestorage filtration of blood components appears to be an effective method to reduce leukocyte-induced adverse reactions and other complications. To determine whether it is better to filter whole blood before component separation, we compared the efficiency of in-line filtration of whole blood with that of postseparation filtration. Methods: Blood was collected from normal, healthy donors into either regular triple-bag containers or into whole-blood integral- filter container systems. We then compared the in vitro storage values of leukocyte-depleted red blood cell concentrates (RBCC) kept at 4°C, and plasma frozen for 1 year with nonfiltered blood components as control. Results: All counts of white blood cells after filtration were < 1 X106 per unit. For almost all storage parameters no significant differences were found between leukocyte-reduced RBCC and control units. The plasma fibrinopeptide A values below 30 ng/ ml prior to freezing indicate that filtration does not activate the coagulation factors. Furthermore, the filtration did not influence either the biological values or the coagulation factors of plasma units. Conclusions: Whole blood filtration prior to component preparation seems to offer a useful alternative technique for obtaining leukocyte-reduced RBCC and plasma.

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Possibility to Improve Preservation of Whole Blood by Leukocyte-Depletion before Storage

Cited by 10 publications

References 12 publications

Prestorage Leukocyte Depletion with In-Line Filtration of Whole Blood in Comparison with Blood Component Leukocyte Depletion

Prestorage Leukocyte Depletion with In-Line Filtration of Whole Blood in Comparison with Blood Component Leukocyte Depletion

The effect of leucocyte depletion on the quality of fresh‐frozen plasma

Prestorage Leukocyte Reduction with In-Line Filtration of Whole Blood: Evaluation of Red Cells and Plasma Storage

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