Prestorage Leukocyte Reduction
with In-Line Filtration of Whole
Blood: Evaluation of Red Cells and
Plasma Storage

Rapaille, A.; Moore, George A.; Flament, Jocelyne; Sondag-Thull, D

doi:10.1159/000461896

Cited by 22 publications

(32 citation statements)

References 32 publications

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“…In the study of human blood, air was gently expressed back into the primary bag after filtration had stopped, allowing more blood to filter. 20 A similar manipulation could have decreased the blood losses in this study. Blood lost to filtration must be considered in the processing of blood components because drawing an additional volume from the donor may be necessary to ensure that the final products are the appropriate volume.…”

Section: Discussionmentioning

confidence: 76%

“…20 Filtration took an average of 7.0 minutes when the blood was filtered immediately after collection, and 15.6 minutes when the blood had been stored at 4ЊC before filtration ( Table 1). The temperature of the blood did not affect the amount of blood lost to the filter, which was approximately 52 mL.…”

Section: Resultsmentioning

confidence: 99%

“…First, leukocytes rapidly go through apoptosis and degradation in storage and thereby release cytokines (interleukin-1, interleukin-6, interleukin-8, tumor necrosis factor-␣) 1 and other inflammatory mediators (histamine, serotonin, and acid phospatase). 20 With transfusion into the patient, these molecules can lead to FNTRs. Second, FNTRs also can be due to allommunization against leukocyte antigens (histocompatability leukocyte antigen [HLA] class I) that are present on platelets and leukocytes.…”

Section: Discussionmentioning

confidence: 99%

“…1,9,19 Leukocyte metabolites such as cytokines, histamine, serotonin, elastase, and acid phosphatase can contribute to transfusion reactions and increase the percentage of hemolysis in stored RBCs. 20 Transfusion reactions due to leukocytes have become such a concern in human transfusion medicine that many medical centers advocate the universal use of leukoreduction filters for all blood products. Although leukocyte-mediated transfusion reactions may occur in dogs, the use of leukoreduction filters on canine blood has not been investigated previously.…”

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confidence: 99%

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Use of a Prestorage Leukoreduction Filter Effectively Removes Leukocytes from Canine Whole Blood While Preserving Red Blood Cell Viability

Brownlee

Wardrop

Sellon

et al. 2000

Veterinary Internal Medicne

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Leukoreduction of blood products is a technique used to prevent leukocyte-induced transfusion reactions. Filters currently used for human blood products achieve at least a 99.9% reduction in leukocyte numbers per unit (450 mL) of blood. Goals of this study were to determine if a prestorage leukoreduction filter could effectively achieve leukoreduction of canine blood and to determine if viability of the leukoreduced red blood cell (RBC) product could be maintained after 35 days of storage. Blood collected from each dog was filtered through a leukoreduction filter at either room temperature or after cooling (4ЊC) for 4 hours. Filtration efficacy was determined by measurement of pre-and postfiltration leukocyte counts. In vitro viability of RBCs was determined by comparing RBC adenosine triphosphate concentration and percent hemolysis before and after the storage period. In vivo viability of stored cells was determined using a biotin-streptavidin-phycoerythrin labeling technique and flow cytometry. Blood filtered within 30 minutes of collection versus blood filtered after cooling had mean reductions in leukocyte numbers of 88.90 and 99.99%, respectively. The mean ATP and hemoglobin concentrations from the in vitro analysis were comparable to those obtained in previously for canine RBC adequately stored for 35 days. The mean in vivo 24-hour survival of the stored RBC was 84.7%. The leukoreduction filter used did not adversely affect in vitro or in vivo viability of canine RBCs. The filter effectively removed leukocytes from blood, with maximal efficiency of filtration achieved with use of cooled blood.

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Section: Discussionmentioning

confidence: 76%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Use of a Prestorage Leukoreduction Filter Effectively Removes Leukocytes from Canine Whole Blood While Preserving Red Blood Cell Viability

Brownlee

Wardrop

Sellon

et al. 2000

Veterinary Internal Medicne

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mentioning

confidence: 99%

Use of a Prestorage Leukoreduction Filter Effectively Removes Leukocytes from Canine Whole Blood While Preserving Red Blood Cell Viability

Brownlee¹,

Wardrop²,

Sellon³

et al. 2000

J Vet Int Med

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Leukoreduction of blood products is a technique used to prevent leukocyte-induced transfusion reactions. Filters currently used for human blood products achieve at least a 99.9% reduction in leukocyte numbers per unit (450 mL) of blood. Goals of this study were to determine if a prestorage leukoreduction filter could effectively achieve leukoreduction of canine blood and to determine if viability of the leukoreduced red blood cell (RBC) product could be maintained after 35 days of storage. Blood collected from each dog was filtered through a leukoreduction filter at either room temperature or after cooling (4C) for 4 hours. Filtration efficacy was determined by measurement of pre-and postfiltration leukocyte counts. In vitro viability of RBCs was determined by comparing RBC adenosine triphosphate concentration and percent hemolysis before and after the storage period. In vivo viability of stored cells was determined using a biotin-streptavidin-phycoerythrin labeling technique and flow cytometry. Blood filtered within 30 minutes of collection versus blood filtered after cooling had mean reductions in leukocyte numbers of 88.90 and 99.99%, respectively. The mean ATP and hemoglobin concentrations from the in vitro analysis were comparable to those obtained in previously for canine RBC adequately stored for 35 days. The mean in vivo 24-hour survival of the stored RBC was 84.7%. The leukoreduction filter used did not adversely affect in vitro or in vivo viability of canine RBCs. The filter effectively removed leukocytes from blood, with maximal efficiency of filtration achieved with use of cooled blood.

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Leukozytendepletion

Lefèvre¹,

Walther-Wenke²,

Burkhard³

1999

Bundesgesundheitsblatt Gesundheitsforschung Gesundheitsschutz

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Prestorage Leukocyte Reduction with In-Line Filtration of Whole Blood: Evaluation of Red Cells and Plasma Storage

Cited by 22 publications

References 32 publications

Use of a Prestorage Leukoreduction Filter Effectively Removes Leukocytes from Canine Whole Blood While Preserving Red Blood Cell Viability

Use of a Prestorage Leukoreduction Filter Effectively Removes Leukocytes from Canine Whole Blood While Preserving Red Blood Cell Viability

Use of a Prestorage Leukoreduction Filter Effectively Removes Leukocytes from Canine Whole Blood While Preserving Red Blood Cell Viability

Leukozytendepletion

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