Possible Interactions between the Biosynthetic Pathways of Indole Glucosinolate and Auxin

Malka, Siva Kumar; Cheng, Youfa

doi:10.3389/fpls.2017.02131

Cited by 71 publications

(59 citation statements)

References 190 publications

(306 reference statements)

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“…, and TRYPTOPHAN SYNTHASE α1 were chosen that are involved in tryptophan synthesis, an IAA precursor, and CYP79B2, CYP79B3, NITRILASE1 (NIT1), and NIT2 implicated in the indole-3-acetaldoxime pathway (Malka and Cheng 2017). The expression of all seven selected genes was repressed in the shoot upon ANE treatments.…”

Section: Molecular Analysis Of Ane-induced Plant Growth Promotion In mentioning

confidence: 99%

Toward the molecular understanding of the action mechanism of Ascophyllum nodosum extracts on plants

et al. 2019

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The importance of biostimulants, defined as plant growth-promoting agents that differ notably from fertilizers, is increasing steadily because of their potential contribution to a worldwide strategy for securing food production without burdening the environment. Based on folkloric evidence and ethnographic studies, seaweeds have been useful for diverse human activities through time, including medicine and agriculture. Currently, seaweed extracts, especially those derived from the common brown alga Ascophyllum nodosum, represent an interesting category of biostimulants. Although A. nodosum extracts (abbreviated ANEs) are readily used because of their capacity to improve plant growth and to mitigate abiotic and biotic stresses, fundamental insights into how these positive responses are accomplished are still fragmentary. Generally, the effects of ANEs on plants have been attributed to their hormonal content, their micronutrient value, and/or the presence of alga-specific polysaccharides, betaines, polyamines, and phenolic compounds that would, alone or in concert, bring about the observed phenotypic effects. However, only a few of these hypotheses have been validated at the molecular level. Transcriptomics and metabolomics are now emerging as tools to dissect the action mechanisms exerted by ANEs. Here, we provide an overview of the available in planta molecular data that shed light on the pathways modulated by ANEs that promote plant growth and render plants more resilient to diverse stresses, paving the way toward the elucidation of the modus operandi of these extracts.

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Section: Molecular Analysis Of Ane-induced Plant Growth Promotion In mentioning

confidence: 99%

Toward the molecular understanding of the action mechanism of Ascophyllum nodosum extracts on plants

et al. 2019

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“…Our quantification analysis demonstrated that the contents of IAA and IAA‐Glc, IAA‐Asp, I3C, oxIAA, and IAN significantly increased in yucca‐d (Figure ). This observation provided insight into the regulation of the biosynthetic pathway of IAAs caused by their over‐accumulation. In contrast, IAA‐Ala and Trp were similarly accumulated in the WT and yucca‐d plants, whereas GBR, a metabolite derived from tryptophan, decreased in yucca‐d (Figure ).…”

Section: Resultsmentioning

confidence: 89%

Practical optimization of liquid chromatography/mass spectrometry conditions and pretreatment methods toward the sensitive quantification of auxin in plants

Sugahara

Kitao

Yamagaki

et al. 2020

Rapid Comm Mass Spectrometry

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Rationale The plant hormone auxin, indole‐3‐acetic acid, regulates many aspects of plant growth and development. Auxin quantification should offer broad insights into its mechanistic action in plants. However, limited auxin content in plant tissues hampers the establishment of quantification methods without the highest graded instruments or deeply specialized experimental techniques. Methods In this study, we detailed optimized conditions for high‐performance liquid chromatography coupled with triple‐quadrupole mass spectrometry (LC/MS). We compared LC/MS conditions, such as columns, mobile phases, parameters of acquisition methods (selective or multiple ion monitoring), dwell times (DTs), and channel numbers, using differentially mixed authentic auxin and its related compounds. We further investigated pretreatment methods through the optimization of auxin recovery and irrelative compound removal from plant tissues prior to the LC/MS analysis. Results Our LC/MS analysis demonstrated the particular importance of the column, DTs, and channel numbers on detection sensitivity. Our comparative analysis developed optimal pretreatment methods, including the pulverization of plants, concentration of extract through centrifugal evaporation, and removal of irrelative metabolites using liquid–liquid extraction and a spin filter. We injected plant samples into our LC/MS system, quantified auxin and eight related compounds in a single measurement, and determined the auxin increase in an auxin over‐producing mutant. Conclusions Our practical optimization of LC/MS conditions and pretreatment methods provides detailed experimental processes toward the sensitive quantification of auxin from 10 mg of plant tissue. These processes have not always been clearly documented; therefore, our protocol could broadly contribute to technical advances in plant growth and development research.

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“…These TFs function in regulation of GLS biosynthesis while MYB 51 involved in indole GLS biosynthesis and MYB76 involved in aliphatic GLS biosynthesis. They increase insect resistance in crops (Malka and Cheng 2017) and can be considered as two key regulatory TFs in resistance to insect attacks (Gigolashvili et al 2009) in cotton, as G. hirsutum and G. raimondii show more resistance to such stress. However, further expression analysis to disclose their function in response to insect attack in cotton species is necessary.…”

Section: Insect Related Transcription Factors Are Common Between the mentioning

confidence: 99%

Transcription factors and molecular markers revealed asymmetric contributions between allotetraploid Upland cotton and its two diploid ancestors

et al. 2020

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Three Gossypium species have been used to breed cotton as they vary in their fiber production and resistance to stresses. Transcription factors (TFs) mostly are present in different copies or isoforms by which they conduct their regulation. Their copy number can determine organism behavior to a cue. Simple sequence repeats (SSRs) are one of the most informative and versatile molecular markers. Transcription factors of three Gossypium species were compared in silico. Seventy eight percent of TFs were common between the three species. Single copy for each species were 6057 TF. Gossypium hirsutum and G. raimondii shared the most common interspecific TF. Gossypium arboreum species-specific TF were the least. MYB TF family with its subfamilies is the most abundant followed by bHLH and AP2/ERF family. Gossypium hirsutum generally possesses more TF copies compared to other two species. The 2109 single-copy clusters indicate that G. hirsutum has received one copy from only one parent. The five most abundant SSR markers of TF were dinucleotides AT, TA, TC, CT and TG belonging to G. raimondii. For G. arboreum and G. hirsutum they were trinucleotides CAA, CGA, TGA, GAA (CAT: G. hirsutum) and TCA. The findings suggest that there is regulatory difference between the three Gossypium species for fiber production and insect attack response. The differences may be due to some adaptive deletion events during speciation of G. hirsutum from its parents G. arboreum and G. raimondii.

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Possible Interactions between the Biosynthetic Pathways of Indole Glucosinolate and Auxin

Cited by 71 publications

References 190 publications

Toward the molecular understanding of the action mechanism of Ascophyllum nodosum extracts on plants

Toward the molecular understanding of the action mechanism of Ascophyllum nodosum extracts on plants

Practical optimization of liquid chromatography/mass spectrometry conditions and pretreatment methods toward the sensitive quantification of auxin in plants

Transcription factors and molecular markers revealed asymmetric contributions between allotetraploid Upland cotton and its two diploid ancestors

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