Possible involvement of Class III phosphatidylinositol-3-kinase in meiotic progression of porcine oocytes beyond germinal vesicle stage

Park, Myung Rae; Gupta, Mukesh Kumar; Lee, Hye Ran; Das, Ziban Chandra; Uhm, Sang Jun; Lee, Hoon Taek

doi:10.1016/j.theriogenology.2010.11.002

Cited by 11 publications

(4 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This experiment was arranged in a 2 × 2 factorial design to determine the effect of temperature (38.5 or 41 °C) and autophagy inhibitor (0 or 10 mM 3MA) during IVM on autophagy activity by measuring LC3 protein level (autophagy marker). COCs were distributed on the following experimental groups: Control (38.5 °C for 22 h) and Heat Shock (41 °C for 16 h followed by 38.5 °C for 6 h 36 , 37 ), in the presence of 0 or 10 mM 3MA during IVM 38 . After IVM, COCs were denuded so that denuded oocytes and respective cumulus cells were processed for western blotting analysis to determine autophagy activity by LC3-I and LC3-II level.…”

Section: Methodsmentioning

confidence: 99%

Autophagy is a pro-survival adaptive response to heat shock in bovine cumulus-oocyte complexes

Latorraca

Feitosa

Mariano

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Autophagy is a physiological mechanism that can be activated under stress conditions. However, the role of autophagy during oocyte maturation has been poorly investigated. Therefore, this study characterized the role of autophagy on developmental competence and gene expression of bovine oocytes exposed to heat shock (HS). Cumulus-oocyte-complexes (COCs) were matured at Control (38.5 °C) and HS (41 °C) temperatures in the presence of 0 and 10 mM 3-methyladenine (3MA; autophagy inhibitor). Western blotting analysis revealed that HS increased autophagy marker LC3-II/LC3-I ratio in oocytes. However, there was no effect of temperature for oocytes matured with 3MA. On cumulus cells, 3MA reduced LC3-II/LC3-I ratio regardless of temperature. Inhibition of autophagy during IVM of heat-shocked oocytes (3MA-41 °C) reduced cleavage and blastocyst rates compared to standard in vitro matured heat-shocked oocytes (IVM-41 °C). Therefore, the magnitude of HS detrimental effects was greater in the presence of autophagy inhibitor. Oocyte maturation under 3MA-41 °C reduced mRnA abundance for genes related to energy metabolism (MTIF3), heat shock response (HSF1), and oocyte maturation (HAS2 and GREM1). In conclusion, autophagy is a stress response induced on heat shocked oocytes. inhibition of autophagy modulated key functional processes rendering the oocyte more susceptible to the deleterious effects of heat shock. Autophagy is a programmed lysosomal process that degrades damaged or unnecessary cellular proteins, lipids, DNA, RNA, and organelles for recycling amino acids, fatty acids, and nucleosides to act as cellular building blocks for anabolic processes 1-3. This event is involved in development, differentiation, immunity, aging, and cell death 2. The autophagy pathway can be triggered by different stress conditions in order to restore homeostasis as a prosurvival adaptive response to stress. For example, autophagy can be induced by diseases 4-6 and environmental factors such as nutrient deprivation 7,8 , high temperature 9-11 , oxidative stress 12,13 , hypoxia 14,15 , and toxins 16-18. There are three autophagy types described in mammalian cells: chaperone-mediated autophagy, macroautophagy, and microautophagy 1. In the chaperone-mediated autophagy, heat shock cognate 70 complex (Hsc70 complex) directs abnormal proteins that have KFERQ-like motifs exposed to lysosome-mediated degradation. On the other hand, during microautophagy the lysosome membrane invaginates, thus capturing a small portion of the cytoplasm to be degraded 2. Macroautophagy is the best-understood type of autophagy, which is mediated by double-membrane vesicles that transport abnormal proteins and organelles through the cytoplasm (i.e., autophagosomes). The autophagosome membrane fuses to lysosomes to breakdown the transported material 2. During embryonic development, autophagy is first induced after fertilization to clear sperm-born material that is not necessary for further embryogenesis 19-21. In turn, autophagy is also associated with abundance of maternal...

show abstract

Section: Methodsmentioning

confidence: 99%

Autophagy is a pro-survival adaptive response to heat shock in bovine cumulus-oocyte complexes

Latorraca

Feitosa

Mariano

et al. 2020

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Understanding oocyte developmental competence remains a key challenge for reproductive biology. As a very important microenvironment for the development of oocytes, FF can provide nutrients and signal stimulus regulation of the folliculogenesis process as well as oocyte growth and development [ 10 ]. FF contains growth factors, electrolytes, hormones, amino acids, and unknown factors, with selective filtration of the blood enriched by secretions from the few cells present in the follicle.…”

Section: Discussionmentioning

confidence: 99%

Follicular fluid C3a-peptide promotes oocyte maturation through F-actin aggregation

Yang,

Zhao,

Chen

et al. 2023

BMC Biol

View full text Add to dashboard Cite

Background Immature cumulus-oocyte complexes are retrieved to obtain mature oocytes by in vitro maturation (IVM), a laboratory tool in reproductive medicine to obtain mature oocytes. Unfortunately, the efficiency of IVM is not satisfactory. To circumvent this problem, we therefore intended to commence with the composition of ovarian follicular fluid (FF), an important microenvironment influencing oocyte growth. It is well known that FF has a critical role in oocyte development and maturation. However, the components in human FF remain largely unknown, particularly with regard to small molecular peptides. Results In current study, the follicular fluid derived from human mature and immature follicles were harvested. The peptide profiles of FF were further investigated by using combined ultrafiltration and LC–MS/MS. The differential peptides were preliminary determined by performing differentially expressed analysis. Human and mouse oocyte culture were used to verify the influence of differential peptides on oocyte development. Constructing plasmids, cell transfecting, Co-IP, PLA etc. were used to reveal the detail molecular mechanism. The results from differentially expressed peptide as well as cultured human and mouse oocytes analyses showed that highly conserved C3a-peptide, a cleavage product of complement C3a, definitely affected oocytes development. Intriguingly, C3a-peptide possessed a novel function that promoted F-actin aggregation and spindle migration, raised the percentage of oocytes at the MII stage, without increasing the chromosome aneuploidy ratio, especially in poor-quality oocytes. These effects of C3a-peptide were attenuated by C3aR morpholino inhibition, suggesting that C3a-peptide affected oocytes development by collaborating with its classical receptor, C3aR. Specially, we found that C3aR co-localized to the spindle with β-tubulin to recruit F-actin toward the spindle and subcortical region of the oocytes through specific binding to MYO10, a key regulator for actin organization, spindle morphogenesis and positioning in oocytes. Conclusions Our results provide a new perspective for improving IVM culture systems by applying FF components and also provide molecular insights into the physiological function of C3a-peptide, its interaction with C3aR, and their roles in enabling meiotic division of oocytes.

show abstract

“…We quantified degrees of cumulus cell expansion in COCs on a scale of 0–2 after 44 h of IVM as described [ 60 , 61 ]. Degrees of 0, 1, and 2, respectively, indicated no expansion or detachment of cumulus cells from the oocyte, moderate expansion between degrees 0 and 2, and full expansion including the corona radiata.…”

Section: Methodsmentioning

confidence: 99%

Effect of Passage Number of Conditioned Medium Collected from Equine Amniotic Fluid Mesenchymal Stem Cells: Porcine Oocyte Maturation and Embryo Development

Park

et al. 2022

IJMS

View full text Add to dashboard Cite

Oocyte in vitro maturation (IVM) is the most important first step in in vitro embryo production. One prerequisite for the success of IVM in oocytes is to provide a rich culture microenvironment that meets the nutritional needs of developing oocytes. We applied different equine amniotic fluid mesenchymal stem cell conditioned medium (eAFMSC-CM) from passages 7, 18, and 27 to porcine oocytes during IVM to determine its effects on oocyte development and subsequent embryo development, specifically. The eAFMSC-CM from passage 7 (eAFMSC-CMp7) has a considerable impact on 9 genes: BAX, BCL2, SOD2, NRF2, TNFAIP6, PTGS2, HAS2, Cx37, and Cx43, which are associated with cumulus cell mediated oocyte maturation. GSH levels and distribution of mitochondrial and cortical granules were significantly increased in oocytes incubated with eAFMSC-CMp7. In addition, catalase and superoxide dismutase activities were high after IVM 44 h with eAFMSC-CMp7. After in vitro fertilization, blastocyst quality was significantly increased in the eAFMSC-CMp7 group compared to control. Lastly, the antioxidant effect of eAFMSC-CMp7 substantially regulated the expression of apoptosis, pluripotency related genes and decreased autophagy activity in blastocysts. Taken together, this study demonstrated that the eAFMSC-CMp7 enhanced the cytoplasmic maturation of oocytes and subsequent embryonic development by generating high antioxidant activity.

show abstract

Possible involvement of Class III phosphatidylinositol-3-kinase in meiotic progression of porcine oocytes beyond germinal vesicle stage

Cited by 11 publications

References 57 publications

Autophagy is a pro-survival adaptive response to heat shock in bovine cumulus-oocyte complexes

Autophagy is a pro-survival adaptive response to heat shock in bovine cumulus-oocyte complexes

Follicular fluid C3a-peptide promotes oocyte maturation through F-actin aggregation

Effect of Passage Number of Conditioned Medium Collected from Equine Amniotic Fluid Mesenchymal Stem Cells: Porcine Oocyte Maturation and Embryo Development

Contact Info

Product

Resources

About