Possible Involvement of Nitric Oxide in Signaling Pigment Dispersion in Teleostean Melanophores

Hayashi, Hiroshi; Fujii, Ryozo

doi:10.2108/zsj.18.1207

Cited by 13 publications

(9 citation statements)

References 25 publications

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“…, we examined the effect of NO inhibitor L-NNA on melanophores, and we found that L-NNA has a melanophore aggregating property. Similar type of results was also obtained by Hayashi and Fujii (2001). L-NNA was found to block the effect of NO donor SNP when both the chemicals are used in combinations.…”

Section: Discussionsupporting

confidence: 81%

“…It has been reported that the NO causes aggregation of melanophores in frog, Xenopus laevis (Nilsson et al 2000), whereas it is responsible for the pigment dispersion in fish, Zacco temminicki and Kryptopterus bicirrhis (Hayashi and Fujii 2001) and crab Chasmagnathus granulates (Vargas et al 2008). The results of these studies are contradictory to each other, which indicate that NO has both excitatory and inhibitory effect on coloration of the pigments in frog and fish, respectively.…”

Section: Introductionmentioning

confidence: 99%

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Nitric oxide inhibited the melanophore aggregation induced by extracellular calcium concentration in snakehead fish, Channa punctatus

Biswas

Palande

Jadhao

2011

Fish Physiol Biochem

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We studied the role of nitric oxide (NO) and extra-cellular Ca(2+) on the melanophores in Indian snakehead teleost, Channa punctatus. Increase of Ca(2+) level in the external medium causes pigment aggregation in melanophores. This pigment-aggregating effect was found to be inhibited when the external medium contained spontaneous NO donor, sodium nitro prusside (SNP) at all the levels of concentration tested. Furthermore, it has been observed that SNP keeps the pigment in dispersed state even after increasing the amount of Ca(2+). In order to test whether NO donor SNP causes dispersion of pigments or not is checked by adding the inhibitor of nitric oxide synthase, N-omega-Nitro-L-arginine (L-NNA) in the medium. It has been noted that the inhibitor L-NNA blocked the effect of NO donor SNP causing aggregation of pigments. In that way NO is inhibiting the effect of extracellular Ca(2+), keeping the pigment dispersed.

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Section: Discussionsupporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Nitric oxide inhibited the melanophore aggregation induced by extracellular calcium concentration in snakehead fish, Channa punctatus

Biswas

Palande

Jadhao

2011

Fish Physiol Biochem

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“…These cells show a dose‐dependent pigment dispersion after treatment with SIN‐1, a potent NO donor. In vertebrates, Hayashi and Fujii (2001) also observed that the increase in NO production induces pigment migration in melanophores of two teleost species treated with various NO donors. In contrast, Oberg et al.…”

Section: Discussionmentioning

confidence: 88%

“…(1996, 1997) found that UV‐irradiated human melanocyte cultures produced NO and that the presence of NO was sufficient to induce melanogenesis. Hayashi and Fujii (2001) reported that NO donors also induce the pigment dispersion in melanophores of teleosts. In other systems, it is known that in insects NO is involved in olfactory signaling (Sapura et al., 2006) and in crustaceans, the expression of NOS has been established in the nerve gland, Y‐organ, gill and gonad (Kim et al., 2004).…”

Section: Introductionmentioning

confidence: 99%

Participation of nitric oxide in the color change induced by UV radiation in the crab Chasmagnathus granulatus

Vargas

Cruz

Maciel

et al. 2008

Pigment Cell Melanoma Res

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The ability of UV radiation to stimulate color change in vertebrates is well known; however, the signaling pathway involved is not fully explained. Since nitric oxide (NO) is among the candidates for this role, in this study the participation of NO signaling in the pigment migration induced by UV radiation in melanophores of the crab Chasmagnathus granulatus was investigated. When the NO donor, SIN-1, was incubated with pieces of epidermis, there was an induction of a dose-dependent pigment dispersion (in vitro assays). When male adults were exposed to different doses of UVA and UVB, N(G)-nitro-l-arginine-methyl-ester, an NO synthase (NOS) blocker produced a decrease of the pigment dispersion induced by UV (in vivo assays). However, in similar assays, 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide, an NO scavenger, decreased only the pigment dispersion induced by UVA. Interestingly, buthionine sulfoximine did not produce any change in pigment dispersion induced by UVA (in vivo assays) and SIN-1 (in vitro assays). Our results using NADPH-diaphorase histochemistry and immunocytochemistry against nNOS indicated the production of NO by epidermal cells. In conclusion, we suggest that NO is a key molecule for the induction of pigment dispersion in the melanophores of Chasmagnthus granulatus, and also that NOS activation is a fundamental step for this process.

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“…The involvement of NO in the induction of melanogenesis was reported, for the first time, in human melanocyte cultures irradiated with UV (12,13). In teleosts, NO donors also induce pigment dispersion in melanophores (14). In crustaceans, the participation of NO in pigment dispersion induced by UV radiation has been demonstrated in tegumental melanophores of the crab Neohelice granulata (10).…”

Section: Introductionmentioning

confidence: 99%

Nitric Oxide‐dependent Pigment Migration Induced by Ultraviolet Radiation in Retinal Pigment Cells of the Crab Neohelice granulata

Filgueira

Guterres

Votto

et al. 2010

Photochem & Photobiology

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The purpose of this study was to verify the occurrence of pigment dispersion in retinal pigment cells exposed to UVA and UVB radiation, and to investigate the possible participation of a nitric oxide (NO) pathway. Retinal pigment cells from Neohelice granulata were obtained by cellular dissociation. Cells were analyzed for 30 min in the dark (control) and then exposed to 1.1 and 3.3 J cm(-2) UVA, 0.07 and 0.9 J cm(-2) UVB, 20 nmβ-PDH (pigment dispersing hormone) or 10 μm SIN-1 (NO donor). Histological analyses were performed to verify the UV effect in vivo. Cultured cells were exposed to 250 μm L-NAME (NO synthase blocker) and afterwards were treated with UVA, UVB or β-PDH. The retinal cells in culture displayed significant pigment dispersion in response to UVA, UVB and β-PDH. The same responses to UVA and UVB were observed in vivo. SIN-1 did not induce pigment dispersion in the cell cultures. L-NAME significantly decreased the pigment dispersion induced by UVA and UVB but not by β-PDH. All retinal cells showed an immunopositive reaction against neuronal nitric oxide synthases. Therefore, UVA and UVB radiation are capable of inducing pigment dispersion in retinal pigment cells of Neohelice granulata and this dispersion may be nitric oxide synthase dependent.

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Possible Involvement of Nitric Oxide in Signaling Pigment Dispersion in Teleostean Melanophores

Cited by 13 publications

References 25 publications

Nitric oxide inhibited the melanophore aggregation induced by extracellular calcium concentration in snakehead fish, Channa punctatus

Nitric oxide inhibited the melanophore aggregation induced by extracellular calcium concentration in snakehead fish, Channa punctatus

Participation of nitric oxide in the color change induced by UV radiation in the crab Chasmagnathus granulatus

Nitric Oxide‐dependent Pigment Migration Induced by Ultraviolet Radiation in Retinal Pigment Cells of the Crab Neohelice granulata

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