Possible Involvement of Oxidative Stress in Salivary Gland of Patients with Sjögren’s Syndrome

Abstract

Objective: To determine the involvement of oxidative stress in the salivary gland of patients with Sjögren’s syndrome (SS). Methods: Oxidative damage to the gland was measured by 8-hydroxy-2′-deoxyguanosine (8-OHdG) and hexanoyl-lysine (HEL) using the SS saliva. In addition, lactate dehydrogenase (LDH) and mitochondrial glutamic-oxaloacetic transaminase (m-GOT), both general markers for cell damage, were also analyzed. Results: Increased levels of 8-OHdG and HEL were found in the saliva of SS patients, but not in that of patients with other salivary gland dysfunction or of healthy individuals. Levels of LDH and m-GOT were significantly correlated with 8-OHdG and HEL levels, respectively. Furthermore, the increased levels of 8-OHdG and HEL were also correlated in the SS saliva. Conclusion: These findings suggested the involvement of oxidative stress in glandular tissue destruction in SS. It was indicated that the detection of 8-OHdG and HEL in the saliva may become a useful tool for the diagnosis of SS.

Relationships between periodontal inflammation, lipid peroxide and oxidative damage of multiple organs in rats

“…In addition, increased levels of tissue 8-OHdG were found in the brain, heart, liver and kidney in the periodontal inflammation model. HEL and 8-OHdG are accepted as parameters of lipid peroxide and oxidative DNA damage, respectively (22). These results suggest that increased blood lipid peroxide following periodontal inflammation could induce oxidative DNA damage of the brain, heart, liver and kidney.…”

“…The purpose of the present study was to examine whether the generation of lipid peroxide in periodontal inflammation could induce oxidative damage in the liver, heart, kidney and brain using a rat model. In order to evaluate lipid peroxide, the level of hexanoyl-lysine (HEL) was determined (22). In addition, the serum level of C-reactive protein (CRP) was measured because previous studies indicated that periodontal inflammation results in low-grade systemic inflammation (7,9,16).…”

Effect of Oxidative Stress on Secretory Function in Salivary Gland Cells

“…Sjögren's syndrome (Fox, 2005;Ryo et al, 2006). These findings suggest that oxidative stress from ROS causes salivary gland dysfunction (Vitolo et al, 2004).…”

Diurnal variations in salivary protein carbonyl levels in normal and cognitively impaired human subjects

“…periodontitis) and systemic (e.g. diabetes, inflammatory bowel disease) conditions (Arana et al 2006;Bahar et al 2007;Celec et al 2005;Gorelik et al 2007;Jahanshahi et al 2004;Kolarzyk et al 2006;Mashayekhi et al 2005;Nagler et al 2000;Reznick et al 2006;Ryo et al 2006;Sawamoto et al 2005;Sculley and Langley-Evans 2003;Sugano et al 2003;Takane et al 2002;Tsai et al 2005). Changes in salivary cortisol secretion patterns (Fiocco et al 2006;Giubilei et al 2001;Hatfield et al 2004;Porter et al 2002;Scherder et al 2006;Wolf et al 2002;Woods and Dimond 2002), amylase activity (Sramek et al 1995), and acetylcholinesterase activity (Sayer et al 2004) have been documented in persons with AD or MCI, attesting to the plausibility of exploiting saliva for neurodegenerative disease biomarker discovery.…”