Possible nonanthropogenic origin of two methanogenic isolates from oil‐producing wells in the san miguelito field, ventura county, California

Ng, TK; Weimer, Paul J.; Gawel, L.J.

doi:10.1080/01490458909377861

Cited by 41 publications

(21 citation statements)

References 10 publications

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“…Of the cultured isolates and enrichment library clones sequenced, seven of the eight unique genera were related to thermophilic microorganisms previously isolated from oil reservoirs, suggesting that these cultivatable thermophiles may be a common component of these geothermally heated specialized subsurface environments. In particular, thermophiles related to the sulfidogenic and fermentative genera Thermotoga, Petrotoga, Thermococcus, and the H 2 -CO 2 -utilizing Methanobacterium and Methanococcus were shown to be widespread within both Californian petroleum systems and worldwide (22,31,42,44).…”

Section: Discussionmentioning

confidence: 99%

“…Physiological types isolated from these biotopes include sulfate reducers (54,58), sulfidogens (31,54), fermentative bacteria (12,22), manganese and iron reducers (23), methanogens (42,52), and acetogens (13). Despite the description of an increasing number of new thermophilic species, relatively little information is available on the composition of microbial assemblages in these unique subsurface environments.…”

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confidence: 99%

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Culture-Dependent and Culture-Independent Characterization of Microbial Assemblages Associated with High-Temperature Petroleum Reservoirs

Orphan

Taylor

Hafenbradl

et al. 2000

Appl Environ Microbiol

453

343

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Recent investigations of oil reservoirs in a variety of locales have indicated that these habitats may harbor active thermophilic prokaryotic assemblages. In this study, we used both molecular and culture-based methods to characterize prokaryotic consortia associated with high-temperature, sulfur-rich oil reservoirs in California. Enrichment cultures designed for anaerobic thermophiles, both autotrophic and heterotrophic, were successful at temperatures ranging from 60 to 90°C. Heterotrophic enrichments from all sites yielded sheathed rods (Thermotogales), pleomorphic rods resembling Thermoanaerobacter, and Thermococcus-like isolates. The predominant autotrophic microorganisms recovered from inorganic enrichments using H 2 , acetate, and CO 2 as energy and carbon sources were methanogens, including isolates closely related to Methanobacterium, Methanococcus, and Methanoculleus species. Two 16S rRNA gene (rDNA) libraries were generated from total community DNA collected from production wellheads, using either archaeal or universal oligonucleotide primer sets. Sequence analysis of the universal library indicated that a large percentage of clones were highly similar to known bacterial and archaeal isolates recovered from similar habitats. Represented genera in rDNA clone libraries included Thermoanaerobacter, Thermococcus, Desulfothiovibrio, Aminobacterium, Acidaminococcus, Pseudomonas, Halomonas, Acinetobacter, Sphingomonas, Methylobacterium, and Desulfomicrobium. The archaeal library was dominated by methanogen-like rDNAs, with a lower percentage of clones belonging to the Thermococcales. Our results strongly support the hypothesis that sulfur-utilizing and methane-producing thermophilic microorganisms have a widespread distribution in oil reservoirs and the potential to actively participate in the biogeochemical transformation of carbon, hydrogen, and sulfur in situ.Over the past decade, microbiological investigations of high temperature, petroleum-rich strata from a number of geographically distant sites have revealed physiologically diverse assemblages of thermophilic and hyperthermophilic anaerobic microorganisms. Physiological types isolated from these biotopes include sulfate reducers (54, 58), sulfidogens (31, 54), fermentative bacteria (12, 22), manganese and iron reducers (23), methanogens (42, 52), and acetogens (13). Despite the description of an increasing number of new thermophilic species, relatively little information is available on the composition of microbial assemblages in these unique subsurface environments. This is primarily due to the reliance on cultured-based methods for the recovery and identification of individual oil field isolates and the focus on specific physiological groups of microorganisms, such as sulfate-reducing and fermentative microorganisms, rather than the entire subsurface microbial community.Culture-based approaches, while extremely useful for understanding the physiological potential of isolated organisms, do not necessarily provide comprehensive information on the comp...

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Culture-Dependent and Culture-Independent Characterization of Microbial Assemblages Associated with High-Temperature Petroleum Reservoirs

Orphan

Taylor

Hafenbradl

et al. 2000

Appl Environ Microbiol

453

343

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“…The methanogens so far isolated and characterized include the (i) hydrogenotro phs : Methanobacterium thermoautrophicum (Ivanov et al, 1983), Methanobacterium bryantii (Davydova-Charakhch'yan et al, 1993a), Methanococcus thermolithotrophicus (Nilsen & Torsvik, 1996), Methanobacterium ivanovii (Belyaev et al, 1983), ' Methanoplanus petrolearius' (Ollivier et al, 1997) and phenotypic variants of Methanobacterium thermoaggregans (Ng et al, 1989) and Methanobacterium thermoalcaliphilum (Davydova-Charakhch'yan et al, 1993a) ; (ii) methylotrophs, ' Methanohalophilus euhalobius ' (Davidova et al, 1997;Obraztsova et al, 1987a), Methanosarcina siciliae (Ni & Boone, 199 1 ;Ni et al, 1994); and (iii) an acetotroph, Methanosarcina mazei (Obraztsova et al, 1987b Methanocalculus halotolerans gen. nov., sp. nov. 11 Data from Romesser et al (1979).…”

Section: Discussionmentioning

confidence: 99%

“…constitute an important microbiological community inhabiting oilfields (Bhupathiraju & McInerney, 1993 ;Ivanov et al, 1983;Ng et al, 1989;Ni & Boone, 1991 ; Nilsen Obraztsova et al, 1987a, b). However, very few studies have reported on methanogens from saline oilfields.…”

Section: Introductionmentioning

confidence: 99%

Methanocalculus halotolerans gen. nov., sp. nov., isolated from an oil-producing well

Ollivier

Fardeau

Cayol

et al. 1998

International Journal of Systematic Bacteriology

132

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Two irregular coccoid methanogens designated SEBR 4845T and FRlT were isolated from an oilfield in Alsace, France. Strain SEBR 4845l (T = type strain) is a hydrogenotrophic halotolerant methanogen, which grows optimally at 5 O/ O NaCl (whr) and tolerates up t o 12O/0 NaCI. It does not use methylated compounds and therefore cannot be ascribed to any of the known genera of the halophilic methylotrophic methanogens. It differs from hydrogenotrophic members of the orders Methanococcales and Methanomicrobiales in the NaCl growth range (0-12% NaCI), which is the widest reported t o date for any hydrogenotrophic methanogen. 165 rRNA gene sequence analysis indicated that strain SEBR 4845l is a novel isolate for which a new genus is proposed, Methanocalculus halotolerans gen. nov., sp. nov. (= OCM 4703 that might be indigenous to the oilfield ecosystem. Strain FRlT (= OCM 471) is a moderately halophilic methanogen which grows optimally at 10% NaCl and tolerates up t o 20% NaCI. It grows on trimethylamine and methanol as carbon and energy sources. The G+C content of its DNA is 43 mol%. It is therefore phenotypically and genotypically related t o members of the genus Methanohalophilus. This report provides evidence that methylotrophic and hydrogenotrophic, but not aceticlastic methanogens are present in a saline subsurface oilfield environment, as already observed in surface saline t o hypersaline environments.

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“…Some oil-bearing reservoirs are deep seated and include halophilic and thermal environments which are conducive to the growth of thermophilic bacteria. To date, only a few reports describing thermophilic bacteria obtained from such environments have been published, and the strains described include methanogens (11,18,29), sulfate reducers (8,28), elemental sulfur reducers (37), and fermentative bacteria (12,30,33). During microbiological in- this study (17,25,27).…”

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confidence: 99%

Description of Thermoanaerobacter brockii subsp. lactiethylicus subsp. nov., Isolated from a Deep Subsurface French Oil Well, a Proposal To Reclassify Thermoanaerobacter finnii as Thermoanaerobacter brockii subsp. finnii comb. nov., and an Emended Description of Thermoanaerobacter brockii

Cayol

Ollivier

Patel

et al. 1995

International Journal of Systematic Bacteriology

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Possible nonanthropogenic origin of two methanogenic isolates from oil‐producing wells in the san miguelito field, ventura county, California

Cited by 41 publications

References 10 publications

Culture-Dependent and Culture-Independent Characterization of Microbial Assemblages Associated with High-Temperature Petroleum Reservoirs

Culture-Dependent and Culture-Independent Characterization of Microbial Assemblages Associated with High-Temperature Petroleum Reservoirs

Methanocalculus halotolerans gen. nov., sp. nov., isolated from an oil-producing well

Description of Thermoanaerobacter brockii subsp. lactiethylicus subsp. nov., Isolated from a Deep Subsurface French Oil Well, a Proposal To Reclassify Thermoanaerobacter finnii as Thermoanaerobacter brockii subsp. finnii comb. nov., and an Emended Description of Thermoanaerobacter brockii

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