Possible Processes for Origin of First Chemoheterotrophic Microorganisms with Modeling of Physiological Processes of Bacterium Bacillus subtilis as a Model System in 2H2O

Ignatov, Ignat; Mosin, Oleg

doi:10.13187/ejmb.2015.9.131

Cited by 18 publications

(22 citation statements)

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“…Two other protons at C2(C3) and C4 positions in ribose residue could be replaced with deuterium via further enzimatic isomerization of ribulose-5-monophosphate into ribose-5-monophosphate. In general, our studies confirmed this scheme [27]. However, it should be noted that the level of deuterium enrichment of inosine molecule is determined by isotopic purity of 2 H 2 O and deuterated substrates and, therefore, for the total administration of the deuterium label into the inosine molecule instead of protonated glucoce it must be used its deuterated analogue.…”

Section: The Studying Of the Level Of Deuterium Enrichment Of [ 2 H]isupporting

confidence: 84%

Deuterated Methylotrophic Biomass as a Substrate for Microbiological Synthesis of 2H-Labeled Purine Ribonucleoside Inosine by Chemoheterotrophic Bacterium Bacillus Subtilis B-3157

Mosin¹,

Ignatov²,

State³

2015

European Reviews of Chemical Research

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We propose to use the hydrolyzed deuterated biomass of the facultative methylotrophic bacterium Brevibacterium methylicum B-5662 as a source of 2 H-labeled growth substrates for microbiological synthesis of 2 H-labeled purine ribonucleoside inosine, excreted into the liquid microbial culture (LC) by a Gram-positive chemoheterotrophic bacterium Bacillus subtilis B-3157. The bacterium was grown on heavy water (HW) medium with 2% (v/v) hydrolysate of deuterated biomass of the methylotrophic bacterium Brevibacterium methylicum B-5662 obtained on minimal salt media M9 supplemented with 2% (v/v) [ 2 H]methanol and increasing gradient of 2 Н 2 O concentration from 0; 24,5; 49,0; 73,5 up to 98% (v/v) 2 Н 2 O. Isolation of 2 H-labeled inosine from LC was performed by adsorption/desorption on activated carbon with following extraction by 0,3 M ammonium-formate buffer (pH = 8,9), crystallization in 80% (v/v) EtOH, and ion exchange chromatography (IEC) on a column with AG50WX 4 cation exchange resin equilibrated with 0,3 M ammonium-formate buffer and 0,045 M NH 4 Cl. The investigation of deuterium incorporation into the inosine molecule by FAB method demonstrated incorporation of 5 deuterium atoms into the molecule (the total level of deuterium enrichment -65,5 atom% 2 H) with 3 deuterium atoms being included into the ribose and 2 deuterium atoms -into the hypoxanthine residue of the molecule.

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Section: The Studying Of the Level Of Deuterium Enrichment Of [ 2 H]isupporting

confidence: 84%

Deuterated Methylotrophic Biomass as a Substrate for Microbiological Synthesis of 2H-Labeled Purine Ribonucleoside Inosine by Chemoheterotrophic Bacterium Bacillus Subtilis B-3157

Mosin¹,

Ignatov²,

State³

2015

European Reviews of Chemical Research

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“…The main stretching band in liquid water is shifted to a lower fequency (v 3 = 3480 cm -1 and v 1 = 3270 cm -1 ), while the bending frequency (v 2 = 1640 cm -1 ) increased by hydrogen bonding [38] Increased strength of hydrogen bonding shifts the stretch vibration to lower frequencies with greatly increased intensity in the infrared due to the increased dipoles. With raising the temperature the stretch vibrations shift to higher frequency, while the intramolecular vibrations shift to lower frequencies [39]. Hydrogen bond energy lies in the range of 2,3 kcal/mole for the N-H...O-bonds up to 7,0 kcal/mol for the bonds with hydrogen fluoride F-H...F. The strength of the hydrogen bonding depends on the cooperative/anticooperative character of the surrounding hydrogen bonds with the strongest hydrogen bonds giving the lowest vibrational frequencies.…”

Section: Methods Of Study Of Water Clustersmentioning

confidence: 99%

Structural Models of Water and Ice Regarding the Energy of Hydrogen Bonding

Ignatov¹,

Mosin²

2015

Nanotechnology Research and Practice

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In this review it is reported about the research on the structure of water and ice and intermolecular water cyclic associates (clusters) with general formula (Н 2 О) n and their charged ionic clusters [(Н 2 О) n ] + and [(Н 2 О) n ] -by means of computer modelling and spectroscopy methods as 1 Н-NMR, IR-spectroscopy, DNES, EXAFS-spectroscopy, X-Ray and neurons diffraction. The computer calculation of polyhedral nanoclusters (Н 2 О) n , where n = 3-20 are carried out. Based on this data the main structural mathematical models describing water structure (quasicrystalline, continious, fractal, fractal-clathrate) have been examined and some important physical characteristics were obtained. The average energy of hydrogen bonding (∆E H…O ) between Н 2 О molecules in the process of cluster formation was measured by the DNES method compiles -0,1067±0,0011 eV. It was also shown that water clusters formed from D 2 О were more stable, than those ones from Н 2 О due to isotopic effects of deuterium.

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“…Thus, the most sensitive to replacement of Н on 2 H are the apparatus of biosynthesis of macromolecules and a respiratory chain, i.e., those cellular systems using high mobility of protons and high speed of breaking up of hydrogen bonds. Last fact allows consider adaptation to 2 H 2 O as adaptation to the nonspecific factor affecting simultaneously the functional condition of several numbers of cellular systems: metabolism, ways of assimilation of carbon substrates, biosynthetic processes, and transport function, structure and functions of macromolecules [27].…”

Section: Studying Of the Level Of Deuterium Enrichment Of [ 2 H]inosinementioning

confidence: 99%

Studying the Biosynthesis of 2H-labeled purine Ribonucleoside Inosine by a Chemoheterotrophic Bacterium Bacillus subtilis B-3157

2015

European Reviews of Chemical Research

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We studied the biosynthesis of 2 H-labeled purine ribonucleoside inosine excreted into liquid microbial culture (LC) by Gram-positive chemoheterotrophic bacterium Bacillus subtilis B-3157 while growing of this bacterium on heavy water (HW) medium with 2% (v/v) hydrolysate of deuterated biomass of the methylotrophic bacterium Brevibacterium methylicum B-5662 as a source of 2 H-labeled growth substrates. Isolation of 2 H-labeled inosine from the LC was performed by adsorption/desorption on activated carbon with following extraction by 0,3 M ammoniumformate buffer (pH = 8,9), crystallization in 80% (v/v) EtOH, and ion exchange chromatography (IEC) on a column with AG50WX 4 cation exchange resin equilibrated with 0,3 M ammoniumformate buffer and 0,045 M NH 4 Cl. The investigation of deuterium incorporation into the inosine molecule by FAB method demonstrated the incorporation of 5 deuterium atoms into the molecule (the total level of deuterium enrichment -65,5 atom% 2 H) with 3 deuterium atoms included into the ribose and 2 deuterium atoms -into the hypoxanthine residue of the molecule. Three nonexchangeable deuterium atoms were incorporated into the ribose residue owing to reactions of enzymatic izomerization of glucose in 2 H 2 O-medium. These non-exchangeable deuterium atoms in the ribose residue were originated from glycolysis, associated with the Embden-Meyerhof pathway, while two other deuterium atoms at C2,C8-positions in the hypoxanthine residue were synthesized from [ 2 H]amino acids that originated from the deuterated hydrolysate of the methylotrophic bacterium Brevibacterium methylicum B-5662. However, the effect of auxotrophy of this strain in tyrosine, histidine, adenine and uracilб presupposes the branched metabolic pathways, different those indicated above.

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Possible Processes for Origin of First Chemoheterotrophic Microorganisms with Modeling of Physiological Processes of Bacterium Bacillus subtilis as a Model System in 2H2O

Cited by 18 publications

References 0 publications

Deuterated Methylotrophic Biomass as a Substrate for Microbiological Synthesis of 2H-Labeled Purine Ribonucleoside Inosine by Chemoheterotrophic Bacterium Bacillus Subtilis B-3157

Deuterated Methylotrophic Biomass as a Substrate for Microbiological Synthesis of 2H-Labeled Purine Ribonucleoside Inosine by Chemoheterotrophic Bacterium Bacillus Subtilis B-3157

Structural Models of Water and Ice Regarding the Energy of Hydrogen Bonding

Studying the Biosynthesis of 2H-labeled purine Ribonucleoside Inosine by a Chemoheterotrophic Bacterium Bacillus subtilis B-3157

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