Possible promotion of neuronal differentiation in fetal rat brain neural progenitor cells after sustained exposure to static magnetism

Nakamichi, Noritaka; Ishioka, Yukichi; Hanabusa, Takao; Ozawa, Shusuke; Terabe, Masaki; Nakamura, Nobuhiro; Takarada, Takeshi; Yoneda, Yukio

doi:10.1002/jnr.22087

Cited by 25 publications

(17 citation statements)

References 49 publications

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“…The increase in Tuj1 expression resulted in a decrease in GFAP labeled cells but not in a decrease of non-neuronal cells. This is consistent with studies that have shown that proneural genes including Math1 bias neuronal differentiation at the expense of glial differentiation [32][33][34].…”

Section: Discussionsupporting

confidence: 92%

Conditional Induction of Math1 Specifies Embryonic Stem Cells to Cerebellar Granule Neuron Lineage and Promotes Differentiation into Mature Granule Neurons

et al. 2013

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Directing differentiation of embryonic stem cells (ESCs) to specific neuronal subtype is critical for modeling disease pathology in vitro. An attractive means of action would be to combine regulatory differentiation factors and extrinsic inductive signals added to the culture medium. In this study, we have generated mature cerebellar granule neurons by combining a temporally controlled transient expression of Math1, a master gene in granule neuron differentiation, with inductive extrinsic factors involved in cerebellar development. Using a Tetracyclin-On transactivation system, we overexpressed Math1 at various stages of ESCs differentiation and found that the yield of progenitors was considerably increased when Math1 was induced during embryonic body stage. Math1 triggered expression of Mbh1 and Mbh2, two target genes directly involved in granule neuron precursor formation and strong expression of early cerebellar territory markers En1 and NeuroD1.Three weeks after induction, we observed a decrease in the number of glial cells and an increase in that of neurons albeit still immature. Combining Math1 induction with extrinsic factors specifically increased the number of neurons that expressed Pde1c, Zic1, and GABAa6R characteristic of mature granule neurons, formed ''T-shaped'' axons typical of granule neurons, and generated synaptic contacts and action potentials in vitro. Finally, in vivo implantation of Math1-induced progenitors into young adult mice resulted in cell migration and settling of newly generated neurons in the cerebellum. These results show that conditional induction of Math1 drives ESCs toward the cerebellar fate and indicate that acting on both intrinsic and extrinsic factors is a powerful means to modulate ESCs differentiation and maturation into a specific neuronal lineage. STEM CELLS 2013;31:652-665 Disclosure of potential conflicts of interest is found at the end of this article.

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Section: Discussionsupporting

confidence: 92%

Conditional Induction of Math1 Specifies Embryonic Stem Cells to Cerebellar Granule Neuron Lineage and Promotes Differentiation into Mature Granule Neurons

et al. 2013

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“…Several in vitro studies [Hirai and Yoneda, ; Nakamichi et al, ] reported that sustained exposure to SMF could facilitate cellular functionality and differentiation in immature neurons and neural progenitor cells. However, this beneficial effect was obtained at the cost of a suppressed proliferation for self‐renewal, which absolutely is not advantageous for neural regeneration.…”

Section: Discussionmentioning

confidence: 99%

Early exposure of rotating magnetic fields promotes central nervous regeneration in planarian Girardia sinensis

Chen

Lin

et al. 2016

Bioelectromagnetics

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Magnetic field exposure is an accepted safe and effective modality for nerve injury. However, it is clinically used only as a supplement or salvage therapy at the later stage of treatment. Here, we used a planarian Girardia sinensis decapitated model to investigate beneficial effects of early rotary non-uniform magnetic fields (RMFs) exposure on central nervous regeneration. Our results clearly indicated that magnetic stimulation induced from early RMFs exposure significantly promoted neural regeneration of planarians. This stimulating effect is frequency and intensity dependent. Optimum effects were obtained when decapitated planarians were cultured at 20 °C, starved for 3 days before head-cutting, and treated with 6 Hz 0.02 T RMFs. At early regeneration stage, RMFs exposure eliminated edema around the wound and facilitated subsequent formation of blastema. It also accelerated cell proliferation and recovery of neuron functionality. Early RMFs exposure up-regulated expression of neural regeneration related proteins, EGR4 and Netrin 2, and mature nerve cell marker proteins, NSE and NPY. These results suggest that RMFs therapy produced early and significant benefit in central nervous regeneration, and should be clinically used at the early stage of neural regeneration, with appropriate optimal frequency and intensity.

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“…Applied electromagnetic fields (EMFs) inhibit proliferation and promote differentiation of mouse bone marrow mesenchymal stem cells (Wu et al 2005) and embryonic stem cells (Ventura et al 2005), and stimulate the maturation and differentiation of cerebellar granule neurons in newborn rat (Lisi et al 2005). Nakamichi and colleagues showed that exposure to a 100 mT static magnetic field (SMF) reduced proliferation of NPCs in the fetal rat brain (Nakamichi et al 2009). Thus, disturbance of the GMF could modulate proliferation and differentiation of NPCs/NSCs.…”

Section: Introductionmentioning

confidence: 99%

Elimination of the geomagnetic field stimulates the proliferation of mouse neural progenitor and stem cells

Liu

et al. 2016

Protein Cell

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Living organisms are exposed to the geomagnetic field (GMF) throughout their lifespan. Elimination of the GMF, resulting in a hypogeomagnetic field (HMF), leads to central nervous system dysfunction and abnormal development in animals. However, the cellular mechanisms underlying these effects have not been identified so far. Here, we show that exposure to an HMF (<200 nT), produced by a magnetic field shielding chamber, promotes the proliferation of neural progenitor/stem cells (NPCs/NSCs) from C57BL/6 mice. Following seven-day HMF-exposure, the primary neurospheres (NSs) were significantly larger in size, and twice more NPCs/NSCs were harvested from neonatal NSs, when compared to the GMF controls. The self-renewal capacity and multipotency of the NSs were maintained, as HMF-exposed NSs were positive for NSC markers (Nestin and Sox2), and could differentiate into neurons and astrocyte/glial cells and be passaged continuously. In addition, adult mice exposed to the HMF for one month were observed to have a greater number of proliferative cells in the subventricular zone. These findings indicate that continuous HMF-exposure increases the proliferation of NPCs/NSCs, in vitro and in vivo. HMF-disturbed NPCs/NSCs production probably affects brain development and function, which provides a novel clue for elucidating the cellular mechanisms of the bio-HMF response.Electronic supplementary materialThe online version of this article (doi:10.1007/s13238-016-0300-7) contains supplementary material, which is available to authorized users.

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Possible promotion of neuronal differentiation in fetal rat brain neural progenitor cells after sustained exposure to static magnetism

Cited by 25 publications

References 49 publications

Conditional Induction of Math1 Specifies Embryonic Stem Cells to Cerebellar Granule Neuron Lineage and Promotes Differentiation into Mature Granule Neurons

Conditional Induction of Math1 Specifies Embryonic Stem Cells to Cerebellar Granule Neuron Lineage and Promotes Differentiation into Mature Granule Neurons

Early exposure of rotating magnetic fields promotes central nervous regeneration in planarian Girardia sinensis

Elimination of the geomagnetic field stimulates the proliferation of mouse neural progenitor and stem cells

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