Possible role of nicotine and cotinine on nitroxidative stress and antioxidant content in saliva of smokeless tobacco consumers

Begum, Shaik Fareeda; Nagajothi, G; Latha, Kodidela Swarna; Sandeep, Gandham; Sreekanth, Bandi; Kumar, Chitta Suresh; Rajendra, Wudayagiri; Maddu, Narendra

doi:10.1016/j.plabm.2018.e00105

Cited by 14 publications

(17 citation statements)

References 36 publications

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“…The final enzyme activity was calculated by normalizing the results to the total protein concentration of the whole protein extract. Catalase (CAT) was determined by the method and the activity of CAT is expressed as µmoles of H 2 O 2 decomposed/min/mg protein or U/mg protein [ 17 ].…”

Section: Methodsmentioning

confidence: 99%

Sirtuin 3 deficiency aggravates contrast-induced acute kidney injury

Zhang¹,

Liu²,

Li³

et al. 2018

J Transl Med

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BackgroundSirtuin 3 (Sirt3) is a key regulator of energy metabolism and oxidative stress. To investigate the role of Sirt3 in contrast-induced acute kidney injury (CIAKI), we established the model both in vivo and in vitro to explore the potential mechanisms.MethodsIn vivo, we established CIAKI models in wild-type (WT) and Sirt3-knockout (Sirt3-KO) mice. Blood urea nitrogen (BUN) and serum creatinine (Scr) were detected by enzyme-linked immunosorbent assay, Glomerular Filtration Rate (GFR) and creatinine clearance were also investigated. We detected the production of reactive oxygen species (ROS) via 2′7′-dichlorodihydro-fluorescein diacetate. The expressions of Sirt3, oxidative stress and apoptosis related markers (MnSOD, Catalase, Acetyl-MnSOD K68, Nox4, Bax, Bcl-2 and Caspase3) were measured and analyzed. In addition, we observed the effect of nicotinamide riboside (NR) on CIAKI in WT and Sirt3-KO mice. In vitro, Sirt3 was knocked out by siRNA transfection method in HK-2 cells. Sirt3, ROS, oxidative stress and apoptosis markers in HK-2 cells were also measured.ResultsOur data demonstrated that the levels of Scr and BUN in Sirt3-KO mice were increased while the levels of the GFR and creatinine clearance were decreased in CIAKI mice. In Sirt3-KO or siRNA groups, the activities of MnSOD and Catalase were markedly down-regulated. Also, the expression of Caspase3 were markedly increased and the ratio of Bcl-2/Bax was decreased, while the ROS level was increased in Sirt3 deficiency groups. NR ameliorated CIAKI in WT mice but not in Sirt3-KO mice.ConclusionOur results suggest that Sirt3 deficiency aggravates contrast-induced acute kidney injury. Sirt3 is critical in NR-mediated renoprotection in CIAKI.

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Section: Methodsmentioning

confidence: 99%

Sirtuin 3 deficiency aggravates contrast-induced acute kidney injury

Zhang¹,

Liu²,

Li³

et al. 2018

J Transl Med

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“…There were no significant correlations found between above said all parameters in tobacco non-chewers (controls). Previous similar studies reported positive correlations in cotinine and lipid peroxide while negative correlations of cotinine and enzymatic antioxidants (Begum, S.F. et al, 2018, Augusta, N., et al, 2018.…”

Section: Discussionmentioning

confidence: 79%

Effect of vitamin C supplementation on serum Cotinine, MDA and vitamin C levels in smokeless Tobacco chewers

Thorat

Joshi

Sontakke

et al. 2019

ijrps

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Smokeless tobacco is a commonly used drug in the world. Tobacco use in any form is a rising crisis for the population in developing countries. Tobacco contributes significantly to oxidative stress induced morbidity and mortality. The study was aimed to evaluate the effect of vitamin C supplementation on serum cotinine, malondialdehyde (MDA) and serum vitamin C level in smokeless tobacco (ST) chewers. In addition, the study was aimed to evaluate the effect of duration of tobacco chewing on above-said parameters. A total number of 338 healthy participants of aged between 31 to 60 years were classified into two groups comprising of tobacco chewers and tobacco non-chewers. Tobacco chewers group further divided into three subgroups with respect to tobacco chewing duration. Participants were asked to take 1000 mg of vitamin C daily for 45 days. Serum cotinine, MDA and serum vitamin C were measured in all the participants before and after supplementation of vitamin C. Serum cotinine (p<0.001) and MDA (p<0.001) were significantly increased whereas serum vitamin C level (p<0.001) was significantly decreased in smokeless tobacco chewers as than to controls. However, serum cotinine (p<0.001) and MDA (p<0.001) were significantly decreased whereas vitamin C (p<0.001) level was significantly increased in both the groups after supplementation of vitamin C as compared to before. It was observed that the changes become more prominent with increased smokeless tobacco consumption duration. Daily intake of vitamin C may be beneficial to restore the oxidant-antioxidant balance in tobacco chewers.

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“…In addition to epigenetic mechanisms, SLTrelated carcinogenesis can also be induced by promoter methylation of tumor suppressor genes, which results in abnormal cell proliferation (4,6). The use of tobacco products over a long time causes increased oxidative stress and the formation of reactive oxygen species (ROS) due to the reaction with biological macromolecules (5,16,21,25,26).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Salivary Lipid Peroxidation in Paan Users

Farhad-Mollashahi

Dalirsani

Nakhaee

et al. 2022

Int J High Risk Behav Addict

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Background: One known risk factor for oral cancer is chewing smokeless tobacco, which is common in southeastern Iran. Smokeless tobacco-related oral complications can be evaluated by assessing parameters of oxidative stress. Objectives: This study evaluated salivary malondialdehyde (MDA) levels in paan users and compared them with nonusers. Patients and Methods: In this study, saliva was collected from 50 paan users and 50 nonusers referred to the Zahedan Dental School in Zahedan, Iran. Salivary MDA was assessed based on standard biochemical methods, and the data were analyzed using a t-test and analysis of variance. Results: In the paan users and the controls, the mean MDA was 2.19 ± 1.05 and 1.24 ± 0.62 (nmol/mL), respectively, and both groups showed significant differences (P < 0.001). The daily consumption and duration were significantly correlated with the MDA concentration, respectively (r = 0.722, P < 0.001), (r = 0.764, P < 0.001). Conclusions: In paan users, salivary MDA concentrations were higher. The concentration of salivary MDA increased with the consumption of paan in more significant quantities and for a more extended time.

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Possible role of nicotine and cotinine on nitroxidative stress and antioxidant content in saliva of smokeless tobacco consumers

Cited by 14 publications

References 36 publications

Sirtuin 3 deficiency aggravates contrast-induced acute kidney injury

Sirtuin 3 deficiency aggravates contrast-induced acute kidney injury

Effect of vitamin C supplementation on serum Cotinine, MDA and vitamin C levels in smokeless Tobacco chewers

Evaluation of Salivary Lipid Peroxidation in Paan Users

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