Possible sources of methane-derived carbon for chironomid larvae

Eller, Gundula; Deines, Peter; Krüger, Martin

doi:10.3354/ame046283

Cited by 21 publications

(10 citation statements)

References 54 publications

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“…This finally confirms the assumptions based on carbon isotope signatures alone by other authors (Bunn & Boon 1993, Grey et al 2004a, b, Kelly et al 2004). Furthermore, this also supports the observation that higher numbers of Eubacteria (including MOB) rather than Archaea (including methanogens) are associated with chironomid larvae (Eller et al 2007, this issue).…”

Section: Discussionsupporting

confidence: 81%

Linking larval chironomids to methane: seasonal variation of the microbial methane cycle and chironomid δ13C

Deines

Grey

Richnow³

et al. 2007

Aquat. Microb. Ecol.

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Section: Discussionsupporting

confidence: 81%

Linking larval chironomids to methane: seasonal variation of the microbial methane cycle and chironomid δ13C

Deines

Grey

Richnow³

et al. 2007

Aquat. Microb. Ecol.

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“…An independent analysis of the larval gut as possible microhabitat for MOB revealed anoxic conditions throughout the gut, which prevent active growth of MOB as part of the gut microflora (Stief and Eller, 2006). The analysis of lake‐derived chironomid larvae by clone libraries for the eubacterial 16S rRNA gene from larval tissue and gut content provided further evidence that MOB are not symbionts of the larvae (Eller et al ., 2007). Furthermore, in experiment 1 only sediment‐associated MOB were provided as 13 C‐enriched food source, but even if the whole larval gut would have been filled up with sediment‐associated MOB, the measured larval δ 13 C signatures could not have exceeded the sediment signature as they did (Table 1).…”

Section: Resultsmentioning

confidence: 99%

Methane‐derived carbon flows through methane‐oxidizing bacteria to higher trophic levels in aquatic systems

Deines

Bodelier

Eller

2007

Environmental Microbiology

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Recent investigations have shown that biogenic methane can be a carbon source for macro invertebrates in freshwater food webs. Stable carbon isotopic signatures, used to infer an organism's food source, indicated that methane can play a major role in the nutrition of chironomid larvae. However, the pathway of methane-derived carbon into invertebrate biomass is still not confirmed. It has been proposed that chironomid larvae ingest methane-oxidizing bacteria (MOB), but this has not been experimentally demonstrated to date. Using (13)C-labelled methane we could show for the first time that chironomid larvae assimilate methane-derived carbon through MOB. Chironomid larval biomass was significantly (13)C-enriched after dwelling for 10 days in lake sediment enriched with labelled methane. Moreover, phospholipid fatty acids diagnostic for MOB were detected in larval tissue and were significantly (13)C-enriched, which encompasses the (13)C-uptake predicted for a methane-based nutrition. Additionally, chironomid larvae fed on sediment and water-column derived MOB biomass.

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“…The findings of this study demonstrate that endogenous bacterial populations in chironomids are stable. Eller et al (2007), who investigated the possible sources of methane-derived carbon from chironomid larvae, identified clone libraries from larval tissue and gut content. The majority of the identified clones in their study were related to known detoxifying bacteria and belonged to the following genera: Bacteroides, Clostridium, Dysgomonas, Hyphomicrobium , Methylobacillus, Methylobacter , Methylocaldum, and Methylomicrobium .…”

Section: Discussionmentioning

confidence: 99%

Bacterial Community Composition Associated with Chironomid Egg Masses

Senderovich

Halpern

2012

Journal of Insect Science

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Chironomids (Diptera: Chironomidae) are the most widely distributed and often the most abundant insect in freshwater. They undergo a complete metamorphosis of four life stages, of which the egg, larva, and pupae are aquatic and the adult is terrestrial. Chironomid egg masses were found to be natural reservoirs of Vibrio cholerae and Aeromonas species. To expand the knowledge of the endogenous bacterial community associated with chironomid egg masses, denaturing gradient gel electrophoresis and clone analysis of 16S rRNA gene libraries were used in this study. Bacterial community composition associated with chironomid egg masses was found to be stable among different sampling periods. Cloned libraries of egg masses revealed that about 40% of the clones were related to bacteria known to degrade various toxicants. These findings were further supported when bacterial species that showed resistance to different toxic metals were isolated from egg masses and larval samples. Chironomids are found under a wide range of water conditions and are able to survive pollutants. However, little is known about their protective mechanisms under these conditions. Chironomid egg masses are inhabited by a stable endogenous bacterial community, which may potentially play a role in protecting chironomids from toxicants in polluted environments. Further study is needed to support this hypothesis.

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Possible sources of methane-derived carbon for chironomid larvae

Cited by 21 publications

References 54 publications

Linking larval chironomids to methane: seasonal variation of the microbial methane cycle and chironomid δ13C

Linking larval chironomids to methane: seasonal variation of the microbial methane cycle and chironomid δ13C

Methane‐derived carbon flows through methane‐oxidizing bacteria to higher trophic levels in aquatic systems

Bacterial Community Composition Associated with Chironomid Egg Masses

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