Very sensitive procedures were developed for the parullel determination of intestinal lactase (LPH) activity and the cognate mRNA. Between 14 and 20 weeks of gestation, lactase activity is low and varies only slightly; at 37 weeks, a relatively high level of activity is observed. The amounts of LPH mRNA correlates with the enzymatic activity (r = 0.64). Culture of fetal jejunal explants for 5 days induces by itself a 2.fold increase in LPI-I mRNA, without any significant change in lactase enzymatic activity. This increase may reflect the loss ofa negative transcriptional regulation opcldtive in vivo, and suggests an additional post-transcriptional regulatory componenl, The addition of hydrocortisone (50 n&/ml) during culture induces a doubling of lactuse activity without variation in LHP mRNA, indicating a post-transcriptional modulation by hydrocortisone, The intestinal lysosomal ucid &guluctosidase activity wus shown to bc unaffected by hydrocortisone treatment. This observation clearly illustrates that the two intestinal P~galactosidascs are regulated differently. Our results suggests a complex developmental regulation of human intestinal lactase and [hut the perinatal increase in lactase activity could be modulated at a post-transcriptional level by hydrocortisone.