Possible Uses of Micro-organisms in the Manufacture of Plastics and Synthetic Fibres

Griffin, Martin; Magor, Ann M.

doi:10.1080/02648725.1986.10647829

Cited by 5 publications

(2 citation statements)

References 49 publications

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“…Biodegradability, non-toxicity and special mechanical properties makes P H B an interesting raw material for some high added-value applications (Griffin and Magor 1986). One of the most studied microorganisms for PHB production is the bacterium Alcaligenes eutrophus, which is known to accumulate high PHB levels on fructose under restricted growth conditions (Hughes and Richardson 1984).…”

Section: Introductionmentioning

confidence: 99%

Production of poly-β-hydroxybutyrate by Alcaligenes eutrophus on different carbon sources

Linko¹,

Vaheri²,

Seppälä³

1993

Appl Microbiol Biotechnol

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Poly-fl-hydroxybutyrate was produced in shake cultures by Alcaligenes eutrophus H16 on fructose, xylose, and fumaric, itaconic, lactic and propionic acids in a three-stage process. The maximum polymer concentration of 6.9 g 1-1 (69% of cell dry matter) was obtained with 20 g 1-1 of fructose with a volumetric productivity of about 0.22 g 1-1 h -1 at 24 h. Up to about 3 g 1 -1 (about 50°70 of cell dry matter) of polymer was also produced on lactic and propionic acids as the sole carbon source during the production phase. In multivariate optimization employing an orthogonal 23-factorial central composite experimental design with fructose as the substrate in a single-stage process, the optimal initial fructose concentration decreased from 3 5 g 1 -1 to 2 4 g 1 -1 when the incubation time was increased from about 35 h to 96 h. The optimal shaking speed range was 90-113 rpm.strains able to produce PHB on glucose have also been isolated (Holmes et al. 1983;King 1982).Little systematic data has been published on the accumulation of PHB by A. eutrophus H16 on different carbon sources. In the present work the production of polyhydroxyalkanoates (PHA) by A. eutrophus on fructose, fumaric acid, itaconic acid, lactic acid, propionic acid, and xylose was studied in shake-flask cultures. Further, a multivariate optimization experiment was carried out with fructose as the carbon source. Materials and methods MicroorganismA. eutrophus H16 (ATCC 17699) was maintained on nutrient agar slants at 5 ° C, and subcultured monthly.

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Section: Introductionmentioning

confidence: 99%

Production of poly-β-hydroxybutyrate by Alcaligenes eutrophus on different carbon sources

Linko¹,

Vaheri²,

Seppälä³

1993

Appl Microbiol Biotechnol

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“…Most of these proteins fall in one of the following five categories: hormones (eg, human insulin, human growth hormone, calcitonin, somatostatin, relaxin, parahormone, erythropoietin), immunomodulators (eg, interferones, interieukines, colony-stimulating factors), blood products (eg, tissue plasminogen activator, factor VIII, factor IX, human serum albumin, alpha-Iantitrypsin), antitumor agents (tumor necrosis factor, immunotoxins), and vaccines (hepatitis B) (1,(13)(14)(15). Future developments, apart from an increase in the number of protein products, will probably show a spread of rDNA technology to many other areas, such as the synthesis of nonprotein compounds through the construction of whole pathways in living organisms (16), the production of nonprotein biopolymers (17), and the catalysis of specific reactions such as bioleaching in mineral processing (18). These applications are, however, very much at the experimental stage at the moment and have not been considered in this review; instead it is systems for the industrial production of peptides and proteins that have been included.…”

Section: Range Of Productsmentioning

confidence: 99%

Biosafety considerations in industries with production methods based on the use of recombinant deoxyribonucleic acid.

Andrup¹,

Nielsen²,

Kølvraa³

1990

Scand J Work Environ Health

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ANDRUP L, NIELSENBH, K0L VRAAS. Biosafety considerations in industries with production methods based on the use of recombinant deoxyribonucleicacid. Scand J Work Environ Health 1990;16:85-95. Since no occupational accidents or diseases have been attributed specifically to the use of constructions containing recombinant deoxyribonucleicacid (rDNA), this paper evaluates the occupational health risks in industries utilizinggenetically manipulated organisms mainly on the basis of theoretical considerations. Bacteria, filamentous fungi, yeasts, and mammalian cellsin culture are in use. For each of these systems the possible hazards are considered. Concerning microbial production systems, infections are regarded as the main problem, but the risk of infection is considered extremely low. As for cells in culture, only dormant viruses are regarded as problematic, but well-defined production cell lines should not contain such undetected and dangerous viruses. Overall, the additional risks posed by rDNA-modified microorganisms are minor. Only long-term observations can, however, confirm this assumption, and consequently the highest feasible containment measures should still be used in the years to come.

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A critical review and evaluation of bioproduction of organic chemicals

Leeper

Andrews

1991

Appl Biochem Biotechnol

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Possible Uses of Micro-organisms in the Manufacture of Plastics and Synthetic Fibres

Cited by 5 publications

References 49 publications

Production of poly-β-hydroxybutyrate by Alcaligenes eutrophus on different carbon sources

Production of poly-β-hydroxybutyrate by Alcaligenes eutrophus on different carbon sources

Biosafety considerations in industries with production methods based on the use of recombinant deoxyribonucleic acid.

A critical review and evaluation of bioproduction of organic chemicals

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