The results of experiments on studying the effect of the growth regulator Zerebra Agro on the grain yield of spring barley, the chemical composition of plants, the quality of the grain and the amino acid composition of the protein when cultivated according to different predecessors on meadow-chernozem soil in the conditions of the Omsk region are presented. Field studies were carried out in 2014-2016. on the fields of the educational and experimental farm of the Omsk State Agrarian University, and laboratory at the Department of Agrochemistry and Soil Science of Omsk State Agrarian University. Vvariety of spring barley – Gift of Siberia. Predecessors – steam and wheat, agricultural technics – common for the zone The use of the growth regulator Zerebra Agro at a dose of 150 ml/ha made it possible to form an increase in the yield of barley grain when cultivating barley after wheat in steam – 0,27 t/ha and 0,31 t/ha when cultivated with the second crop after steam (in the control variant, the yield was for a pair of 2.82 t/ha, for wheat 1.72 t/ha). The applied growth regulator had a positive effect on the grain quality of spring barley.