Post-column reaction detection system for the determination of organophosphorus compounds by liquid chromatography

Negative ion chemical ionization GC/MS-MS analysis of dialkylphosphate metabolites of organophosphate pesticides in urine of non-occupationally exposed subjects

“…However, after separation using an ion pairing reagent and post-column derivatisation, detection limits in the low mg L 21 levels were achieved by Priebe and Howell. 25 Techniques, such as negative ion thermospray LCMS and LC/MS-MS, have also been used for mg L 21 levels of the metabolites in aqueous solutions. 26 In this paper we report a method of analysis for six DAP metabolites of OP pesticides in urine with significantly improved limits of detection.…”

Biochemical and Toxicological Investigations Related to OP Compounds

“…Many OP insecticides and their metabolites have these characteristics and a variety of HPLC methods have appeared over the years (Brealey and Lawrence, 1979;Abou-Donia and Abou-Donia, 1982;Priebe and Howell, 1985;Unni et al 1992;Ramsteiner and Hormann, 1975;Clark et al, 1985;Szalontai, 1976, Martinez et al, 1992Thapar et al, 1994). Sample preparation can be quite simple.…”

“…Szalontai (1976) used a liquid chromatograph with a transport wire flame ionisation detector to examine the adsorption properties and normal phase separation of 23 OP insecticides on silica gel columns, but there is no record of this method ever being applied to residues or biological materials. One of the most complex means of detection was that invented by Priebe and Howell (1985).…”

Pesticides

“…General Procedures. An HPLC postcolumn reaction detection system for OP compounds based on their degradation to orthophosphate followed by formation of reduced heteropolymolybdate was applied to urine and tomato analysis (281). OP pesticides were screened with triple quadrupole MS (II).…”