Post-Genomic Technologies for the Advancement of Oil Palm Research

Masura, Subhi Siti

doi:10.21894/jopr.2017.00013

Cited by 17 publications

(9 citation statements)

References 16 publications

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“…At the same time, it is important to reduce production costs. In this respect, special attention should be given to the improvement of oil palm varieties with higher yields and good oil quality, and which are compact in architecture, better adapted to climate change and exhibit higher tolerance to diseases [32,33]. Alternative vegetable oils to support the process have been tested, such as coconut oil [34], but their current technology readiness level is still lower with respect to the PKO route.…”

Section: Results From the Biospri Tender Study On Support To Randi Polimentioning

confidence: 99%

The role of biotechnology in the transition from plastics to bioplastics: an opportunity to reconnect global growth with sustainability

et al. 2021

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Section: Results From the Biospri Tender Study On Support To Randi Polimentioning

confidence: 99%

The role of biotechnology in the transition from plastics to bioplastics: an opportunity to reconnect global growth with sustainability

et al. 2021

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“…Thus, in parallel to this research, efforts are focused on achieving the ultimate goal of the oil palm genetic engineering programme to increase the production of oleic acid content in palm oil by manipulating the FAD2 gene of the fatty acid biosynthesis pathway [40][41][42]. In plant, reducing or knocking out the expression of the FAD2 gene will reduce the percentage of linoleic acid and subsequently will increase the level of oleic acid.…”

Section: Discussionmentioning

confidence: 99%

“…Later, this technology will be applied to oil palm with the aim to produce high oleic acid content. For this purpose, we aim to knock out the activities of the two key enzymes regulating oil palm fatty acid composition, namely the FAD2 and palmitoyl-ACP-thioesterase (PAT) genes [40][41][42]. This strategy would greatly reduce the FAD2 and PAT enzyme activities and is expected to increase the oleic acid content to more than 70% with a concomitant decrease of linoleic acid and other longchain polyunsaturated fatty acids.…”

Section: Discussionmentioning

confidence: 99%

“…The Pol III promoter (U6) of oil palm will be used to control the expression of sgRNA since the use of endogenous U6P promoter increased the gene-editing efficiency 4-6-fold as demonstrated in soybean [50] and rice [17]. The oil palm constitutive promoters (UEP1, UEP2, TCTP), which were isolated and characterized previously [41], will also be incorporated in the CRISPR/Cas9 vector to control the Cas9 gene. The strategies are expected to increase the gene-editing efficiency of oil palm significantly.…”

Section: Discussionmentioning

confidence: 99%

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Multiplex CRISPR/Cas9-mediated genome editing of the FAD2 gene in rice: a model genome editing system for oil palm

Bahariah

Masani

Rasid

et al. 2021

Journal of Genetic Engineering and Biotechnology

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Background Genome editing employing the CRISPR/Cas9 system has been widely used and has become a promising tool for plant gene functional studies and crop improvement. However, most of the applied CRISPR/Cas9 systems targeting one locus using a sgRNA resulted in low genome editing efficiency. Results Here, we demonstrate the modification of the FAD2 gene in rice using a multiplex sgRNA-CRISPR/Cas9 genome editing system. To test the system’s efficiency for targeting multiple loci in rice, we designed two sgRNAs based on FAD2 gene sequence of the Oryza sativa Japonica rice. We then inserted the validated sgRNAs into a CRISPR/Cas9 basic vector to construct pYLCRISPRCas9PUbi-H:OsFAD2. The vector was then transformed into protoplast cells isolated from rice leaf tissue via PEG-mediated transfection, and rice calli using biolistic transformation. Direct DNA sequencing of PCR products revealed mutations consisting of deletions of the DNA region between the two target sgRNAs. Conclusion The results suggested that the application of the multiplex sgRNA-CRISPR/Cas9 genome editing system may be useful for crop improvement in monocot species that are recalcitrant to genetic modification, such as oil palm.

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“…In striving effort to uncover the complexities and diverse physiological processes of the oil palm fruit ripening, the multi‐“omics” approach involving genomics, transcriptomics, proteomics and metabolomics platforms is pivotal . Investigation on the oil palm fruit development has been initiated to reveal the compositional, hormonal and transcriptional modifications throughout the developmental phases of oil palm fruit prior to an in‐depth study of ethylene and cell wall‐related processes .…”

Section: Introductionmentioning

confidence: 99%

Deciphering key proteins of oil palm (Elaeis guineensis Jacq.) fruit mesocarp development by proteomics and chemometrics

et al. 2018

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Palm oil is an edible vegetable oil derived from lipid‐rich fleshy mesocarp tissue of oil palm (Elaeis guineensis Jacq.) fruit and is of global economic and nutritional relevance. While the understanding of oil biosynthesis in plants is improving, the fundamentals of oil biosynthesis in oil palm still require further investigations. To gain insight into the systemic mechanisms that govern oil synthesis during oil palm fruit ripening, the proteomics approach combining gel‐based electrophoresis and mass spectrometry was used to profile protein changes and classify the patterns of protein accumulation during these complex physiological processes. Protein profiles from different stages of fruit ripening at 10, 12, 14, 15, 16, 18 and 20 weeks after anthesis (WAA) were analysed by two‐dimensional gel electrophoresis (2DE). The proteome data were then visualised using a multivariate statistical analysis of principal component analysis (PCA) to get an overview of the proteome changes during the development of oil palm mesocarp. A total of 68 differentially expressed protein spots were successfully identified by matrix‐assisted laser desorption/ionisation‐time of flight (MALDI‐TOF/TOF) and functionally classified using ontology analysis. Proteins related to lipid production, energy, secondary metabolites and amino acid metabolism are the most significantly changed proteins during fruit development representing potential candidates for oil yield improvement endeavors. Data are available via ProteomeXchange with identifier PXD009579. This study provides important proteome information for protein regulation during oil palm fruit ripening and oil synthesis.

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Post-Genomic Technologies for the Advancement of Oil Palm Research

Abstract: For a century, Malaysia has been bestowed with oil palm as one of its economic drivers. In the recent decades

Cited by 17 publications

References 16 publications

The role of biotechnology in the transition from plastics to bioplastics: an opportunity to reconnect global growth with sustainability

The role of biotechnology in the transition from plastics to bioplastics: an opportunity to reconnect global growth with sustainability

Multiplex CRISPR/Cas9-mediated genome editing of the FAD2 gene in rice: a model genome editing system for oil palm

Deciphering key proteins of oil palm (Elaeis guineensis Jacq.) fruit mesocarp development by proteomics and chemometrics

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