Post-mortem hemoparasite detection in free-living Brazilian brown brocket deer (Mazama gouazoubira, Fischer 1814)

Silveira, Júlia A.G.; Rabelo, Élida Mara Leite; Lima, Paula Cristina Senra; Chaves, Bárbara Neves; Ribeiro, Múcio Flávio Barbosa

doi:10.1590/s1984-29612014035

Cited by 16 publications

(10 citation statements)

References 37 publications

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“…In Minas Gerais state observation of A. phagocytophilum has been increasing in animals (Silveira et al 2014 and recently, the present study group detected a dog with Ehrlichia canis and A. phagocytophilum co-infection in the city of Belo Horizonte. Lethargy and skin lesions were the clinical signs observed and abnormal hematological parameters such as severe thrombocytopenia were the most important laboratorial alterations .…”

Section: Introductionsupporting

confidence: 53%

“…In Brazil, the bacterium has been detected by molecular methods in dogs (Santos et al 2011, in carnivorous birds ) and in brown brocket deer (Mazama gouazoubira) (Silveira et al 2014); and in horses (Salvagni et al 2010) and Brazilian marsh deer (Blastocerus dichotomus) (Sacchi et al 2012) by serological methods. In Minas Gerais state observation of A. phagocytophilum has been increasing in animals (Silveira et al 2014 and recently, the present study group detected a dog with Ehrlichia canis and A. phagocytophilum co-infection in the city of Belo Horizonte.…”

Section: Introductionmentioning

confidence: 99%

“…Samples from the msp4-positive dogs were submitted to further PCR analyses in which the target was the msp2/p44 gene from A. phagocytophilum and 16S rRNA region of members of the Anaplasmataceae family that infects granulocytes and platelets and monocytes. All PCR assays were performed according to Silveira et al (2014), Zeidner et al (2000) and Lin et al (2003) (Table 1). Purified positive samples were sequenced and analyzed at URL http://asparagin.cenargen.embrapa.br/phph/ and using MEGA 6.0 software (Tamura et al 2013).…”

Section: Introductionmentioning

confidence: 99%

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Important frequency of Anaplasma phagocytophilum infection in a population of domiciled dogs in an urbanized area in south-eastern Brazil

Silveira

Reis²,

Estevam

et al. 2017

Pesq. Vet. Bras.

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958RESUMO.-[Importante frequência da infecção por Anaplasma phagocytophilum em uma população de cães domiciliados em área urbanizada no sudeste do Brasil.] Anaplasma phagocytophilum é responsável pela anaplasmose granulocítica, doença que acomete seres--humanos e várias espécies de animais. O objetivo do presente estudo foi determinar a prevalência de cães acometidos por A. phagocytophlium em uma área residencial de Belo Horizonte, MG, Brasil. Sessenta e dois cães foram submetidos a testes sorológicos (reação de imunofluorescência indireta -IFAT) e moleculares (PCR). Anticorpos anti-A. phagocytophilum foram detectados em 43,8% dos cães. Sete cães (10,9%) foram positivos no PCR para o gene msp4 de A. phagocytophilum, seis para o gene msp2/ p44 A. phagocytophilum e quatro para a região 16S rRNA de Anaplasmataceae granulocíticas. Esse estudo confirma a frequência relativamente alta da infecção por A. phagocytophilum em uma população de cães domiciliados em área urbanizada no sudeste do Brasil e destaca a necessidade de pesquisas para determinar o papel do carrapato Rhipicephalus sanguineus sensu lato na transmissão desse microrganismo para cães de áreas urbanas brasileiras. TERMOS DE INDEXAÇÃO:Anaplasma phagocytophilum, cães, anaplasmose canina, epidemiologia, IFAT, PCR. Important frequency of

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Section: Introductionsupporting

confidence: 53%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Important frequency of Anaplasma phagocytophilum infection in a population of domiciled dogs in an urbanized area in south-eastern Brazil

Silveira

Reis²,

Estevam

et al. 2017

Pesq. Vet. Bras.

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“…These results suggest that there may be possibly a closely related species but not the same as A. phagocytophilum-like agent circulating in Colombia. Although A. phagocytophilum has already been detected in dogs (SANTOS et al, 2011 and brown brocket deer (SILVEIRA et al, 2014) in Brazil, previous reports also have found new genotypes of Anaplasma spp. phylogenetically similar to A. phagocytophilum in wild carnivores kept in captivity in zoos , wild birds and in cats (ANDRÉ et al, 2014) in Brazil, corroborating with the findings of the present study.…”

Section: Discussionmentioning

confidence: 93%

“…Several reports have been published about the occurrence of these agents in South America (DAGNONE et al, 2009;MACHADO et al, 2012;SACCHI et al, 2012;SALVAGNI et al, 2010;SANTOS et al, 2009SANTOS et al, , 2011SANTOS et al, , 2013SILVEIRA et al, 2014;ANDRÉ et al, 2012ANDRÉ et al, , 2014. In Colombia, although a 20% seroprevalence to A. phagocytophilum has been found among rural workers in the country's northern region, the agent has not yet been characterized molecularly in mammals (MÁTTAR & PARRA, 2006).…”

Section: Introductionmentioning

confidence: 99%

Molecular detection of Anaplasma species in dogs in Colombia

Vargas-Hérnandez

André

Cendales

et al. 2016

Rev. Bras. Parasitol. Vet.

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Anaplasma platys and A. phagocytophilum are tick-borne pathogens that parasitize platelets and neutrophils, respectively, of humans and animals. The former is the etiological agent of canine cyclic thrombocytopenia, while the latter is that of canine granulocytic anaplasmosis. This work involved the detection and identification of Anaplasma species in blood samples from dogs in Colombia, using molecular techniques. Between December 2008 and April 2009, blood samples were drawn from the cephalic vein of 91 dogs in the central-western region of Colombia (cities of Bogota, Villavicencio and Bucaramanga) and stored in tubes containing EDTA. These samples were used in 16S rRNA-Anaplasma spp. nPCR and the preparation of blood smears. One (1.1%) of the 91 sampled dogs showed inclusions suggestive of Anaplasmataceae agents in the cytoplasm of platelets. Based on PCR followed by sequencing and phylogenetic analysis, A. platys and Anaplasma sp. closed related to A. phagocytophilum were detected in two and one dog, respectively. Interestingly, all the samples were negative for specific msp-2-A. phagocytophilum real-time qPCR, suggesting the circulation of an Anaplasma species phylogenetically related to A. phagocytophilum in dogs in the aforementioned region. Hence, Anaplasma spp. circulates among dogs in Colombia, albeit with low frequency. To the best of authors' knowledge, this is the first molecular detection of Anaplasma spp. in dogs in Colombia.Keywords: Dogs, Anaplasma spp., Anaplasma platys, Colombia. ResumoAnaplasma platys e A. phagocytophilum são patógenos transmitidos por carrapatos que parasitam plaquetas e neutrófilos, respectivamente, de humanos e animais. Enquanto o primeiro é agente etiológico da trombocitopenia cíclica canina, o último é o agente responsável pela anaplasmose granulocítica canina. O presente trabalho objetivou detectar e caracterizar, utiizando técnicas moleculares, a presença de espécies de Anaplasma em amostras de sague de cães na Colômbia. Entre Dezembro de 2008 e Abril de 2009, amostras de sangue colhidas em tubos contendo EDTA da veia cefálica de 91 cães da região centro-oeste da Colômbia (cidades de Bogotá, Villavicencio e Bucaramanga). As amostras de sangue foram utilizadas em reações de nPCR para 16S rRNA-Anaplasma spp. e confecção de esfregaços sanguíneos. Um (1,1%) dos 91 cães amostrados mostraram inclusões sugestivas de agentes Anaplasmataceae no citoplasma de plaquetas. Baseada na PCR seguida de sequenciamento e análise filogenética, A. platys and Anaplasma sp. relacionado a A. phagocytophilum foram detectados em dois e um cão, respectivamente. Interessantemente, todas as amostras mostraram-se negativas na PCR em tempo real quantitativa específica para msp-2-A. phagocytophilum, sugerindo a circulação de uma espécie de Anaplasma sp. filogeneticamente relacionado ao A. phagocytophilum em cães na região estudada. Embora em uma baixa freqüência, Anaplasma spp. circula entre cães na Colômbia. O presente trabalho representa a primeira detecção de Anaplasma spp. em cães na Colô...

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Report of Theileria luwenshuni and Theileria sp. RSR from cervids in Gansu, China

Liu

et al. 2015

Parasitol Res

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Theileria parasites are important tick-borne pathogens of animals and cause huge economic losses worldwide. Here, we undertook to assess the prevalence of Theileria spp. in cervids in Gansu, China, based on PCR analysis and sequencing of the 18S rRNA genes. Molecular survey showed that Theileria luwenshuni and Theileria sp. RSR were frequently found in Gansu cervids, and the prevalence of T. luwenshuni was 66.7% (n = 9, 6/9) in roe deer in Zhengning County, 58.3% (n = 12, 7/12) in sika deer in Ningxian County, 50% (n = 10, 5/10) in sika deer and 42.9% (n = 14, 6/14) in red deer in Weiyuan County. The prevalence of Theileria sp. RSR was 77.8% (n = 9, 7/9) in roe deer in Zhengning County, 75% (n = 12, 9/12) in sika deer in Ningxian County, 60.0% (n = 10, 6/10) in sika deer, and 50.0% (n = 14, 7/14) in red deer in Weiyuan County. The co-prevalence of the two T. luwenshuni and Theileria sp. RSR was 55.6% (n = 5, 5/9) in roe deer in Zhengning County, 50% (n = 12, 6/12) in sika deer in Ningxian county, 50% (n = 10, 5/10) in sika deer and 35.7% (n = 14, 5/14) in red deer in Weiyuan County, respectively. No other Theileria species was found in these samples. T. luwenshuni and Theileria sp. RSR were detected for the first time in cervids in Gansu, China. Animal experiments showed that four sheep were inoculated with roe deer blood from Zhengning, sika deer blood from Ningxian, sika deer blood, and red deer blood from Weiyuan, respectively, and the Theileria isolated from these inoculated sheep was identified as T. luwenshuni, but Theileria sp. RSR was not detected in these sheep. Our results extend our understanding of the epidemiology of cervine theileriosis in Gansu, China, and will facilitate the implementation of measures to control theileriosis in cervids and small ruminants. This was the first report to demonstrate the occurrence of T. luwenshuni from roe deer, sika deer, and red deer worldwide.

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Post-mortem hemoparasite detection in free-living Brazilian brown brocket deer (Mazama gouazoubira, Fischer 1814)

Cited by 16 publications

References 37 publications

Important frequency of Anaplasma phagocytophilum infection in a population of domiciled dogs in an urbanized area in south-eastern Brazil

Important frequency of Anaplasma phagocytophilum infection in a population of domiciled dogs in an urbanized area in south-eastern Brazil

Molecular detection of Anaplasma species in dogs in Colombia

Report of Theileria luwenshuni and Theileria sp. RSR from cervids in Gansu, China

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