Post-PKS enzyme complexes

Gober, Redding; Wheeler, Ryan; Rohr, Jürgen

doi:10.1039/c9md00066f

Cited by 5 publications

(3 citation statements)

References 64 publications

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“…It is noteworthy that cluster 21 displayed 74% similarity with reference chrysomycin BGC. The biosynthesis of gilvocarcins M and V consisted of two initial substrates (propionyl CoA and acetyl CoA) and nine malonyl CoAs as extending units as well as a series of successive oxidation, reduction, rearrangement, and methylation [ 9 ]. Owing to the same motif of chrysomycin and gilvocarcin, these natural products have similar biosynthetic pathways ( Figure 7 b).…”

Section: Resultsmentioning

confidence: 99%

“…Chrysomycin analogs are one group of glycosides with a benzonaphthopyranone structure obtained from several Streptomyces strains [ 8 , 9 ], which display a broad spectrum of biological properties, including anti-phage, anti-bacterial, and cytotoxic activities [ 10 , 11 , 12 , 13 ]. Especially, chrysomycin A ( Figure 1 ) showed a potent inhibitory effect on multidrug-resistant (MDR) and extensively drug-resistant (XDR) Mycobacterium tuberculosis , methicillin-resistant Staphylococcus aureus (MRSA), and vancomycin-resistant Enterococcus (VRE) [ 13 , 14 , 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

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Comprehensive Genomic Analysis of Marine Strain Streptomyces sp. 891, an Excellent Producer of Chrysomycin A with Therapeutic Potential

Tang

Liu

et al. 2022

Marine Drugs

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Chrysomycin A is one of the most promising therapeutic candidates for treating infections caused by multidrug-resistant Gram-positive bacteria. By hybridizing next-step generation (Illumina) and third-generation (PacBio) sequencing technologies, a high-quality chromosome-level genome together with a plasmid was firstly assembled for chrysomycin A-producing marine strain 891. Phylogenetic analysis of the 16S rRNA gene and genome sequences revealed that this strain unambiguously belonged to the genus Streptomyces, and its genomic features and functional genes were comprehensively analyzed and annotated. AntiSMASH analysis of this strain unveiled one key biosynthetic gene cluster, T2PKS, responsible for the biosynthesis of chrysomycin, the biosynthesis pathway of which was putatively proposed. These findings definitely shed light on further investigation for construction of a robust industrial strain with high-yield chrysomycin A production using genetic engineering techniques and combinatorial biology approaches.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comprehensive Genomic Analysis of Marine Strain Streptomyces sp. 891, an Excellent Producer of Chrysomycin A with Therapeutic Potential

Tang

Liu

et al. 2022

Marine Drugs

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“…The evolutionary origins of fungal biosynthetic pathways indicate natural deviations in gene transcription, DNA repair, gene loss and duplication, and horizontal gene transfer leading to a constant state of chemical flux and expanded chemodiversity 121 . Similarly, many fungal biosynthetic pathways produce more than one natural product, often referred to as intermediates or shunt metabolites, suggesting that combinatorial biosynthesis naturally occurs within the cell 122 . This is further exemplified by branching and converging biosynthetic pathways frequently found in fungi 123 , implying that currently elucidated pathways will continue to evolve and produce variants of known natural products.…”

Section: Discussionmentioning

confidence: 99%

Combinatorial biosynthesis for the engineering of novel fungal natural products

Skellam,

Rajendran,

2024

Commun Chem

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Natural products are small molecules synthesized by fungi, bacteria and plants, which historically have had a profound effect on human health and quality of life. These natural products have evolved over millions of years resulting in specific biological functions that may be of interest for pharmaceutical, agricultural, or nutraceutical use. Often natural products need to be structurally modified to make them suitable for specific applications. Combinatorial biosynthesis is a method to alter the composition of enzymes needed to synthesize a specific natural product resulting in structurally diversified molecules. In this review we discuss different approaches for combinatorial biosynthesis of natural products via engineering fungal enzymes and biosynthetic pathways. We highlight the biosynthetic knowledge gained from these studies and provide examples of new-to-nature bioactive molecules, including molecules synthesized using combinations of fungal and non-fungal enzymes.

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The antitumor antibiotic mithramycin: new advanced approaches in modification and production

Kormanec

Nováková

Csolleiova

et al. 2020

Appl Microbiol Biotechnol

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Post-PKS enzyme complexes

Abstract: This review highlights the protein–protein interactions between type II post-PKS tailoring enzymes with an emphasis on gilvocarcin and mithramycin.

Cited by 5 publications

References 64 publications

Comprehensive Genomic Analysis of Marine Strain Streptomyces sp. 891, an Excellent Producer of Chrysomycin A with Therapeutic Potential

Comprehensive Genomic Analysis of Marine Strain Streptomyces sp. 891, an Excellent Producer of Chrysomycin A with Therapeutic Potential

Combinatorial biosynthesis for the engineering of novel fungal natural products

The antitumor antibiotic mithramycin: new advanced approaches in modification and production

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