Post rapid freezing growth of Antarctic strain of Heterococcus sp. monitored by cell viability and chlorophyll fluorescence

Vitality and growth rate of agar plate-cultivated Antarctic microautotrophs: Analysis of PSII functioning by chlorophyll fluorescence parameters

“…It is consistent with previous studies since in the initial phases of algal cuture growth after innoculation, F S is a proxy of biomass growth (see e.g. Orekhova at al. 2018 for Heterococcus sp.…”

Post Cryopreservation Growth Kinetic and Photosynthetic Assessment of an Acid Tolerant Strain of <i>Stichococcus bacillaris</i>

“…Aliquot of this mixture was observed using the optical microscope approach. Additionally, the chlorophyll a autofluorescence of microalgal cells was inspected under the fluorescence microscope using a epifluorescence microscope Nikon Eclipse E800 [37]. Specifically, these analyses were carried out immediately after thawing.…”

Responses of thallus anatomy and chlorophyll fluorescence-based photosynthetic characteristics of two Antarctic species of genus Usnea to low temperature

“…We hypothesized that the size of structural components of thallus might be changed and injured after fast freezing episodes happening to wet thalli in the field. To evaluate freezing-induced changes of the two Usnea species, we used a rapid freezing approach (see e.g., Orekhova et al 2018) which focuses on anatomical properties and physiological processes in cells surviving a short-term immersion of wet lichen thalli to liquid nitrogen. Moreover, we addressed intraspecific differences in cryoresistance of the two species.…”