Post-stress metabolism involves umbelliferone production in anthocyanin-producing and non-producing cells of Glehnia littoralis suspension cultures

Ishikawa, Aya; Kitamura, Yoshihisa; Ozeki, Yoshihiro; Itoh, Yoshio; Yamada, Akiyo; Watanabe, Masami

doi:10.1016/j.jplph.2004.11.014

Cited by 13 publications

(12 citation statements)

References 26 publications

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“…Linear type furanocoumarins, i.e., psolaren, bergapten and xanthotoxin, are known to be phytoalexins and have been detected after various stress treatments in cell suspension cultures of parsley [2][3][4] and Ammi majus [5]. We also found furanocoumarin production in nonanthocyanin-producing cells but not in anthocyaninproducing cells of G. littoralis after elicitation using yeast extract [6][7][8]. In addition to cell cultures, furanocoumarin induction is reported in seedlings [9] and the leaves of parsley [10] and Apium graveolens [11], though no report on the induction in the root itself has appeared as yet.…”

Section: Introductionmentioning

confidence: 53%

Different responses of shoot and root cultures of Glehnia littoralis to yeast extract

et al. 2006

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Differentiated shoot and root cultures of Glehnia littoralis were examined for organ-specific responses to yeast extract (YE). Growth, and changes in phenylalanine ammonia-lyase (PAL, EC 4. 1. 3. 5) activity, as well as furanocoumarin and simple phenylpropanoid production were then determined. YE affected root growth positively but negatively affected the growth of both the leaf and petiole. PAL activity was induced in all organs and reached a maximum after 2 days of treatment, though the activity in leaves was about three times higher than that in roots. A large amount of p-coumaric acid (p-CA) was transiently excreted into the culture medium of leaves, which has only been rarely reported to date. Subsequently, bergapten and xanthotoxin appeared in the medium. In contrast, no furanocoumarin was detected in the root cultures throughout the course of treatment. Changes in simple phenylpropanoid contents such as p-CA, caffeic acid (CafA) and ferulic acid (FA) in tissues were analyzed in three forms, i.e., free, soluble-conjugated and insoluble-conjugated forms. In leaves, little difference between control and YE-treated tissues was found except free p-CA, but every form of simple phenylpropanoid was increasingly elicited in the roots. These results indicate that YE acts bi-functionally on the root as a nutrient and an elicitor, but only as an elicitor in the leaf.

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Section: Introductionmentioning

confidence: 53%

Different responses of shoot and root cultures of Glehnia littoralis to yeast extract

et al. 2006

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“…Their examination showed that a higher amount of xanthotoxin was detected in the co-culture system than in the single culture system, suggesting that the biosynthetic pathways of the different types of cultures were able to interact with each other. Because our G. littoralis violet cells produce and release umbelliferone into the culture medium (Ishikawa et al 2005), we expected initially that white cells would utilize umbelliferone to produce bergapten, as is the case mentioned above. However, exogenous umbelliferone was not converted to bergapten, although it was taken up by G. littoralis suspension cells.…”

Section: Discussionmentioning

confidence: 98%

“…In the violet cells, on the other hand, the transient suppression of PAL, which is constitutively expressed, caused a decrease in anthocyanin production; however, no bergapten was induced by yeast-extract treatment. Further survey revealed that the violet cells produced umbelliferone and released it into the culture medium continuously, with or without elicitation (Ishikawa et al 2005). Although it is quite hypothetical to compare different cell cultures without experimental evidence, we presumed that in intact plants of G. littoralis, violet cells and white cells probably possess metabolically similar properties to epidermal cells (anthocyanin-producing cells) and their adjacent, parenchymatous cells (furanocoumarin-producing cells), respectively.…”

Section: Introductionmentioning

confidence: 97%

“…Using the white cells, we showed that phenylalanine ammonia-lyase (PAL) was induced de novo by yeast-extract treatment and this was followed by the production of umbelliferone and bergapten in the culture medium, although umbelliferone was only detected transiently (Ishikawa et al 2005). In the violet cells, on the other hand, the transient suppression of PAL, which is constitutively expressed, caused a decrease in anthocyanin production; however, no bergapten was induced by yeast-extract treatment.…”

Section: Introductionmentioning

confidence: 99%

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Constitutive expression of bergaptol O-methyltransferase in Glehnia littoralis cell cultures

et al. 2008

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We investigated whether exogenously supplied precursors of bergapten, namely umbelliferone, psoralen and bergaptol, could be utilized to produce bergapten without elicitation in Glehnia littoralis cell suspension cultures. The levels of added psoralen and bergaptol in the medium soon decreased, and this was followed by the detection of bergapten in both culture fluid and cells.Umbelliferone was also incorporated but in this case no bergapten was produced; instead, skimmin, umbelliferone monoglucoside, was detected. To determine whether conversion of psoralen to bergapten was due to enzyme induction by precursor feeding, the transcript accumulations and enzyme activities of bergaptol O-methyltransferase (BMT, EC 2.1.1.69), which catalyzes the last step of bergapten synthesis, and of phenylalanine ammonia-lyase (PAL, EC 4.3.1.5), which catalyzes the initial step of the phenylpropanoid biosynthetic pathway and is known as a marker enzyme of elicitation, were examined. The results showed that both the expression and the activity of BMT

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“…Furanocoumarin extraction from root tissues and culture media was carried out as previously reported, except for the internal standard used (Ishikawa et al, 2005). As an internal standard, 4-methylumbelliferone (50 L, 5mM MeOH solution) was added to the culture medium before extraction.…”

Section: Extraction and Hplc Analysis Of Furanocoumarin And New Productsmentioning

confidence: 99%

Iron availability alters ascorbate-induced stress metabolism in Glehnia littoralis root cultures

et al. 2012

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Post-stress metabolism involves umbelliferone production in anthocyanin-producing and non-producing cells of Glehnia littoralis suspension cultures

Cited by 13 publications

References 26 publications

Different responses of shoot and root cultures of Glehnia littoralis to yeast extract

Different responses of shoot and root cultures of Glehnia littoralis to yeast extract

Constitutive expression of bergaptol O-methyltransferase in Glehnia littoralis cell cultures

Iron availability alters ascorbate-induced stress metabolism in Glehnia littoralis root cultures

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