Post-transcriptional control of human cytomegalovirus gene expression

Demarchi, J

doi:10.1016/0042-6822(83)90355-0

Cited by 69 publications

(47 citation statements)

References 39 publications

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“…Earlier studies had shown that a 5 kb RNA is transcribed from the IE region of HCMV (DeMarchi, 1983;Jahn et al, 1984a ;Wathen & Stinski, 1982). This RNA spans a short DNA sequence for which in vitro transforming activity has been reported (Nelson et al, 1984).…”

Section: Mapping and Kinetics Of The 5 Kb Rnamentioning

confidence: 99%

“…Here we report on the structure of a fourth gene from the IE region of HCMV that appears to be different from all other IE genes thus far described in herpesviruses. The transcript from this gene, an RNA of about 5 kb, appears to be present in RNA preparations from infected ceils purified at IE, E and late times after infection (DeMarchi, 1983;Jahn et al, 1984a, b;Wathen & Stinski, 1982). It spans a DNA fragment for which in vitro transforming activity has been reported (Nelson et al, 1984).…”

Section: Introductionmentioning

confidence: 99%

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Abundant 5 kb RNA of Human Cytomegalovirus without a Major Translational Reading Frame

Plachter

Traupe²,

Albrecht³

et al. 1988

Journal of General Virology

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SUMMARYAlthough all herpesviruses are similar in their temporal regulation of gene expression, the organization of the immediate early (IE) genes varies markedly between the different members of the group. Most of the IE transcripts of human cytomegalovirus originate from a restricted region within the long unique segment of its linear dsDNA genome of 235 kb. One of the predominant transcripts from the IE region is a 5 kb RNA. Northern blot analyses revealed that this class of RNA is continuously present in infected cells. It was detected at high levels in IE and late RNA preparations, and in low amounts in early RNA preparations. It was not confined to the poly(A) + fraction upon oligo(dT) selection, but also appeared in similar amounts in poly(A)-fractions. Fine mapping of this transcript was done by nuclease protection and primer extension. The RNA appeared to be unspliced, and no signals such as TATA or CCAAT, known to be important elements in eukaryotic RNA polymerase II promoters, were found close to the 5' end. Sequence analysis revealed multiple stop codons throughout the AT-rich potential coding region. Since no splicing was found to occur, the largest protein deduced from the DNA sequence would be of not more than 12000 Mr. However, a computer program designed to detect protein-coding DNA sequences by codon usage did not reveal significant evidence for a protein encoded in this region. Therefore this RNA is likely to represent an unprecedented case of a large non-coding transcript present in cells that are lytically infected by an animal virus.

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Section: Mapping and Kinetics Of The 5 Kb Rnamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Abundant 5 kb RNA of Human Cytomegalovirus without a Major Translational Reading Frame

Plachter

Traupe²,

Albrecht³

et al. 1988

Journal of General Virology

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“…Replication of HCMV is associated with sequential expression of three classes of viral genes: immediate early (IE), early, and late genes (3)(4)(5). Expression of HCMV IE genes can be detected in monocytes from HCMV-infected patients (6)(7)(8).…”

Section: Introductionmentioning

confidence: 99%

Modulation of interleukin 1 beta gene expression by the immediate early genes of human cytomegalovirus.

Iwamoto¹,

Monick²,

Clark³

et al. 1990

J. Clin. Invest.

136

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“…Like other members of the herpesvirus family, the genome of HCMV is temporally expressed during the viral life cycle (16,56,81,88,89). The (16,17,29,33,34,56,64,79,80,82,88,89,91). At least three IE RNAs have been found to be transcribed from these regions (Fig.…”

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confidence: 99%

In vivo and in vitro analysis of transcriptional activation mediated by the human cytomegalovirus major immediate-early proteins.

et al. 1993

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To define mechanistically how the human cytomegalovirus (HCMV) major immediate-early (IE) Human cytomegalovirus (HCMV), a member of the herpesvirus family, is an important pathogen implicated in a variety of diseases in newborn and immunocompromised individuals (for reviews, see references 2 and 59). Like other members of the herpesvirus family, the genome of HCMV is temporally expressed during the viral life cycle (16, 56,81,88,89 (16,17,29,33,34, 56,64,79,80,82,88,89,91). At least three IE RNAs have been found to be transcribed from these regions (Fig. 1B) (62,66,77,80).In previous studies, we and others have used transientexpression assays to demonstrate that HCMV early promoters as well as heterologous viral and cellular promoters can be activated by the region of the genome specifying the IE1 and IE2 gene products (4,5,9,12,15,18,20,23,26,29,39,53,60,63,67,75,85,87). Recent studies suggest that the IE2 86-kDa protein plays a major role in activating HCMV early promoters as well as in repressing its own promoter (the major IE promoter), while the IE1 72-kDa protein acts to enhance the activity of the major IE promoter and may augment the stimulatory effect of the IE2 86-kDa protein (10-12, 26, 28, 50, 53, 61-63, 69, 78).Any consideration of the mechanism by which the HCMV IE1 and IE2 proteins affect transcription must take into account the fact that multiple viral and cellular promoters 1238 on April 27, 2019 by guest

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Post-transcriptional control of human cytomegalovirus gene expression

Cited by 69 publications

References 39 publications

Abundant 5 kb RNA of Human Cytomegalovirus without a Major Translational Reading Frame

Abundant 5 kb RNA of Human Cytomegalovirus without a Major Translational Reading Frame

Modulation of interleukin 1 beta gene expression by the immediate early genes of human cytomegalovirus.

In vivo and in vitro analysis of transcriptional activation mediated by the human cytomegalovirus major immediate-early proteins.

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