Post-transcriptional gene silencing by RNA interference in non-mammalian vertebrate systems: Where do we stand?

Sifuentes-Romero, Itzel; Milton, Sarah L.; García‐Gasca, Alejandra

doi:10.1016/j.mrrev.2011.09.001

Cited by 35 publications

(36 citation statements)

References 152 publications

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“…Precise techniques can then be used to knockout or knockdown these candidates and thus manipulate behaviour (Wong & Hofmann, 2010). For a behavioural ecologist, the utility of a candidate gene approach may be limited for reasons discussed above: behaviour is often regulated by multiple genes of small effect, and genetic knock-downs and knock-outs can be difficult to apply to nonmodel systems, although this is changing with improvements in methodology, especially RNA interference (Ament et al, 2011; Nelson, Ihle, Fondrk, Page, & Amdam, 2007; Sifuentes-Romero, Milton, & Garcia-Gasca, 2011). …”

Section: Gene Expression Profiles: Analytical Toolsmentioning

confidence: 99%

Genomics: moving behavioural ecology beyond the phenotypic gambit

Rittschof

Robinson

2014

Animal Behaviour

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Researchers studying the adaptive significance of behaviour typically assume that genetic mechanisms will not inhibit evolutionary trajectories, an assumption commonly known as the ‘phenotypic gambit’. Although the phenotypic gambit continues to be a useful heuristic for behavioural ecology, here we discuss how genomic methods provide new tools and conceptual approaches that are relevant to behavioural ecology. We first describe how the concept of a genetic toolkit for behaviour can allow behavioural ecologists to synthesize both genomic and ecological information when assessing behavioural adaptation. Then we show how gene expression profiles can be viewed as complex phenotypic measurements, used to (1) predict behaviour, (2) evaluate phenotypic plasticity and (3) devise methods to manipulate behaviour in order to test adaptive hypotheses. We propose that advances in genomics and bioinformatics may allow researchers to overcome some of the logistical obstacles that motivated the inception of the phenotypic gambit. Behavioural ecology and genomics are mutually informative, providing potential synergy that could lead to powerful advances in the field of animal behaviour.

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Section: Gene Expression Profiles: Analytical Toolsmentioning

confidence: 99%

Genomics: moving behavioural ecology beyond the phenotypic gambit

Rittschof

Robinson

2014

Animal Behaviour

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“…The putative targets of IHNV NV-PPM1Bb complexes should be investigated and PPM1Bb gene knockdown in fish cells performed in order to control whether the viruses are still able to efficiently counteract the host antiviral response. Although the available siRNA technology does not work in fish cell lines to efficiently knockdown the expression of cellular genes (for review51), the recent advances in CRISPR/Cas9, adapted to zebrafish and salmon, will offer the opportunity to address the importance of such recruitment for Novirhabdovirus evasion from host innate immunity5253.…”

Section: Discussionmentioning

confidence: 99%

NV Proteins of Fish Novirhabdovirus Recruit Cellular PPM1Bb Protein Phosphatase and Antagonize RIG-I-Mediated IFN Induction

Biacchesi

Mérour

Chevret

et al. 2017

Sci Rep

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Non virion (NV) protein expression is critical for fish Novirhabdovirus, viral hemorrhagic septicemia virus (VHSV) and infectious hematopoietic necrosis virus (IHNV), in vivo pathogenesis. However, the mechanism by which NV promotes the viral replication is still unclear. We developed an approach based on reverse genetics and interactomic and identified several NV-associated cellular partners underlying cellular pathways as potential viral targets. Among these cell partners, we showed that NV proteins specifically interact with a protein phosphatase, Mg2+/Mn2+-dependent, 1Bb (PPM1Bb) and recruit it in the close vicinity of mitochondria, a subcellular compartment important for retinoic acid-inducible gene-I- (RIG-I)-mediated interferon induction pathway. PPM1B proteins belong to the PP2C family of serine/threonine (Ser/Thr) protein phosphatase and have recently been shown to negatively regulate the host antiviral response via dephosphorylating Traf family member-associated NF-κB activator (TANK)-binding kinase 1 (TBK1). We demonstrated that NV proteins and PPM1Bb counteract RIG-I- and TBK1-dependent interferon (IFN) and IFN-stimulated gene promoter induction in fish cells and, hence, the establishment of an antiviral state. Furthermore, the expression of VHSV NV strongly reduced TBK1 phosphorylation and thus its activation. Our findings provide evidence for a previously undescribed mechanism by which a viral protein recruits PPM1Bb protein phosphatase to subvert innate immune recognition.

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“…O silenciamento gênico transcricional é baseado na manipulação de sequências gênicas pela inserção de mutações em regiões específicas do gene, produzindo proteínas truncadas ou proteínas com domínios não funcionais. Já no silenciamento gênico pós-transcricional a repressão da expressão gênica é feita pela degradação do RNA mensageiro (RNAm) (CAPECCHI, 1989;SCHERER;ROSSI, 2003;SIFUENTES-ROMERO;MILTON;GARCÍA-GASCA, 2011 (JORGENSEN et al, 1996;SIFUENTES-ROMERO;MILTON;GARCÍA-GASCA, 2011; VAN DER KROL et al, 1990). Ao mesmo tempo, Guo e Kemphues, ao investigar a função de genes no nemátodo Caenorhabditis elegans, administraram RNA antisense para bloquear a produção da proteína de interesse, obtendo o resultado esperado.…”

Section: Terapia Gênicaunclassified

Sistemas líquido cristalinos de geleificação in situ de administração intratumoral para liberação localizada de siRNA na terapia do câncer de pele

Cardoso¹

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Post-transcriptional gene silencing by RNA interference in non-mammalian vertebrate systems: Where do we stand?

Cited by 35 publications

References 152 publications

Genomics: moving behavioural ecology beyond the phenotypic gambit

Genomics: moving behavioural ecology beyond the phenotypic gambit

NV Proteins of Fish Novirhabdovirus Recruit Cellular PPM1Bb Protein Phosphatase and Antagonize RIG-I-Mediated IFN Induction

Sistemas líquido cristalinos de geleificação in situ de administração intratumoral para liberação localizada de siRNA na terapia do câncer de pele

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