Post-treatments of polydopamine coatings influence cellular response

Davidsen, Maiken Berglund; Teixeira, Jorge Felipe Lima; Dehli, Jeppe; Karlsson, Christian; Kraft, David C.; Souza, Pedro Paulo Chaves de; Foss, Morten

doi:10.1016/j.colsurfb.2021.111972

Cited by 25 publications

(22 citation statements)

References 28 publications

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“…PDA polymerization occurs under simple oxidative alkaline conditions [ 16 ] and effectively mediates the adhesion of cell growth factors and ECM proteins onto various substrates coated with PDA via Michael addition or Schiff base reaction [ 17 , 18 , 19 , 20 ]. In addition, PDA can be used to coat various surfaces, including metals, ceramics, and polymers [ 16 , 21 ]. Moreover, even if the surface of a material is originally hydrophobic, it can be converted to hydrophilic with long-term stability [ 17 , 18 , 19 , 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

“…In addition, PDA can be used to coat various surfaces, including metals, ceramics, and polymers [ 16 , 21 ]. Moreover, even if the surface of a material is originally hydrophobic, it can be converted to hydrophilic with long-term stability [ 17 , 18 , 19 , 21 , 22 ]. Thus, PDA is increasingly being studied and employed in cell research due to its universal good coating performance and ability to adhere to diverse cellular molecules.…”

Section: Introductionmentioning

confidence: 99%

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Fabrication of a Cell-Friendly Poly(dimethylsiloxane) Culture Surface via Polydopamine Coating

et al. 2022

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In this study, we fabricated a poly(dimethylsiloxane) (PDMS) surface coated with polydopamine (PDA) to enhance cell adhesion. PDA is well known for improving surface adhesion on various surfaces due to the abundant reactions enabled by the phenyl, amine, and catechol groups contained within it. To confirm the successful surface coating with PDA, the water contact angle and X-ray photoelectron spectroscopy were analyzed. Human umbilical vein endothelial cells (HUVECs) and human-bone-marrow-derived mesenchymal stem cells (MSCs) were cultured on the PDA-coated PDMS surface to evaluate potential improvements in cell adhesion and proliferation. HUVECs were also cultured inside a cylindrical PDMS microchannel, which was constructed to mimic a human blood vessel, and their growth and performance were compared to those of cells grown inside a rectangular microchannel. This study provides a helpful perspective for building a platform that mimics in vivo environments in a more realistic manner.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Fabrication of a Cell-Friendly Poly(dimethylsiloxane) Culture Surface via Polydopamine Coating

et al. 2022

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“…These results are not surprising, considering the biocompatibility of the used proteins ascribable to their peculiar chemical composition and macro-structure [ 47 ] that both actively influence the in vitro regulation of basic cellular interactions with the substrate.…”

Section: Resultsmentioning

confidence: 99%

Polydopamine-Coated Poly-Lactic Acid Aerogels as Scaffolds for Tissue Engineering Applications

et al. 2022

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Poly-L-lactic acid (PLLA) aerogel-based scaffolds were obtained from physical PLLA gels containing cyclopentanone (CPO) or methyl benzoate (BzOMe) molecules. An innovative single step method of solvent extraction, using supercritical CO2, was used to achieve cylindrical monolithic aerogels. The pore distribution and size, analyzed by SEM microscopy, were found to be related to the crystalline forms present in the physical nodes that hold the gels together, the stable α’-form and the metastable co-crystalline ε-form, detected in the PLLA/BzOMe and PLLA/CPO aerogels, respectively. A higher mechanical compressive strength was found for the PLLA/CPO aerogels, which exhibit a more homogenous porosity. In vitro biocompatibility tests also indicated that monolithic PLLA/CPO aerogels exhibited greater cell viability than PLLA/BzOMe aerogels. An improved biocompatibility of PLLA/CPO monolithic aerogels was finally observed by coating the surface of the aerogels with polydopamine (PDA) obtained by the in situ polymerization of dopamine (DA). The synergistic effect of biodegradable polyester (PLLA) and the biomimetic interface (PDA) makes this new 3D porous scaffold, with porosity and mechanical properties that are tunable based on the solvent used in the preparation process, attractive for tissue engineering applications.

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“… (A) The influence of PDA coating time upon roughness and thickness of the PDA layer on the PGF (reproduced content is open access) ( Felfel et al, 2021 ). (B) Post-treatment of the PDA layer present on the PDA-coated Ti samples and the quantification of fibroblast, HaCaT, and MC3T3-E1 cells on the surfaces (reproduced content is open access) ( Davidsen et al, 2021 ). (C) The different pH values of Tris buffer and their effects on the film thickness illustrated in the comparative chart (I) , mapping chart (II) , and line chart (III) (reproduced content is open access) ( Szewczyk et al, 2022 ).…”

Section: Polydopamine Coating’s Key Process Parameters In Tissue Engi...mentioning

confidence: 99%

“…In both treatments, the numbers of the quinone and primary amine groups increased and decreased, respectively, improving layer stability and favoring cellular proliferation. The proliferation of fibroblast, human keratinocytes (HaCaT), and MC3T3-E1 cells on post-treated PDA-coated samples was more enhanced in comparison with the PDA-coated ones ( Figure 2B ) ( Davidsen et al, 2021 ).…”

Section: Polydopamine Coating’s Key Process Parameters In Tissue Engi...mentioning

confidence: 99%

The effects of process parameters on polydopamine coatings employed in tissue engineering applications

Sarkari

Khajehmohammadi

Davari

et al. 2022

Front. Bioeng. Biotechnol.

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The biomaterials’ success within the tissue engineering field is hinged on the capability to regulate tissue and cell responses, comprising cellular adhesion, as well as repair and immune processes’ induction. In an attempt to enhance and fulfill these biomaterials’ functions, scholars have been inspired by nature; in this regard, surface modification via coating the biomaterials with polydopamine is one of the most successful inspirations endowing the biomaterials with surface adhesive properties. By employing this approach, favorable results have been achieved in various tissue engineering-related experiments, a significant one of which is the more rapid cellular growth observed on the polydopamine-coated substrates compared to the untreated ones; nonetheless, some considerations regarding polydopamine-coated surfaces should be taken into account to control the ultimate outcomes. In this mini-review, the importance of coatings in the tissue engineering field, the different types of surfaces requiring coatings, the significance of polydopamine coatings, critical factors affecting the result of the coating procedure, and recent investigations concerning applications of polydopamine-coated biomaterials in tissue engineering are thoroughly discussed.

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Post-treatments of polydopamine coatings influence cellular response

Cited by 25 publications

References 28 publications

Fabrication of a Cell-Friendly Poly(dimethylsiloxane) Culture Surface via Polydopamine Coating

Fabrication of a Cell-Friendly Poly(dimethylsiloxane) Culture Surface via Polydopamine Coating

Polydopamine-Coated Poly-Lactic Acid Aerogels as Scaffolds for Tissue Engineering Applications

The effects of process parameters on polydopamine coatings employed in tissue engineering applications

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